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The use of ScCO2 for the extraction of LPS from S. enterica subsp. PCM 2266

Rybka, J.; Grycko, P.; da Cruz Francisco, José LU ; Gamian, A. and Dey, Estera LU (2009) 2nd International Conference on Environmental, Industrial and Applied Microbiology In Current Research Topics In Applied Microbiology And Microbial Biotechnology p.460-464
Abstract
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used,... (More)
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
vaccine, Salmonella, lipopolysaccharides (LPS), Supercritical carbon dioxide (scCO(2))
in
Current Research Topics In Applied Microbiology And Microbial Biotechnology
pages
460 - 464
publisher
World Scientific
conference name
2nd International Conference on Environmental, Industrial and Applied Microbiology
external identifiers
  • WOS:000268100200096
language
English
LU publication?
yes
id
1b897095-0346-4727-a980-52584d0a7aac (old id 1461867)
date added to LUP
2009-08-21 13:18:43
date last changed
2016-09-29 10:14:49
@misc{1b897095-0346-4727-a980-52584d0a7aac,
  abstract     = {The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis.},
  author       = {Rybka, J. and Grycko, P. and da Cruz Francisco, José and Gamian, A. and Dey, Estera},
  keyword      = {vaccine,Salmonella,lipopolysaccharides (LPS),Supercritical carbon dioxide (scCO(2))},
  language     = {eng},
  pages        = {460--464},
  publisher    = {ARRAY(0xa5b9e78)},
  series       = {Current Research Topics In Applied Microbiology And Microbial Biotechnology},
  title        = {The use of ScCO2 for the extraction of LPS from S. enterica subsp. PCM 2266},
  year         = {2009},
}