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Protein and polyelectrolyte layer-by-layer films: Assembly and electron transfer

Haberska, Karolina LU (2009)
Abstract
A layer-by-layer deposition technique for assembly of the three-dimensional bioelectrochemical systems, with potential applications such as sensing layers in biosensors or catalytic organisations in biofuel cells, was addressed in this thesis. Several investigations were carried out to understand and control multilayer films formation on planar surfaces, using electrochemical and ellipsometrical methods.

Synthetic and natural/biological polymers were used as building blocks in layer-by-layer films construction. Redox enzymes, such as peroxidases and "blue" multicopper oxidases, were incorporated into multilayer assemblies to provide layers with the catalytic functions. Bioelectrocatalytic activity of the enzymes in the layer... (More)
A layer-by-layer deposition technique for assembly of the three-dimensional bioelectrochemical systems, with potential applications such as sensing layers in biosensors or catalytic organisations in biofuel cells, was addressed in this thesis. Several investigations were carried out to understand and control multilayer films formation on planar surfaces, using electrochemical and ellipsometrical methods.

Synthetic and natural/biological polymers were used as building blocks in layer-by-layer films construction. Redox enzymes, such as peroxidases and "blue" multicopper oxidases, were incorporated into multilayer assemblies to provide layers with the catalytic functions. Bioelectrocatalytic activity of the enzymes in the layer structures was evaluated by measuring direct and mediated electron transfer rates between the enzymes and assembly supporting electrodes. In order to enhance direct electron transfer properties of the enzymes in layer-by layer films, gold nanoparticles and carbon nanotubes were used for nanowiring of the redox enzymes.

It was shown that layer-by-layer deposition techniques enable controlled assembly of nanoparticle-enzyme conjugates on the electrode surfaces. This provided a possibility for evaluating kinetic and thermodynamic characteristics of the heterogeneous electron transfer between the redox enzymes and gold nanoparticles. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Bilewicz, Renata, Faculty of Chemistry, Warsaw University, Poland
organization
publishing date
type
Thesis
publication status
published
subject
keywords
layer-by-layer assembly, nanoparticles, electron transfer, bioelectrocatalysis
defense location
Lecture Hall F, Centre for Chemistry and Chemical Enginering, Sölvegatan 39, Lund
defense date
2009-12-11 10:15
ISBN
978-91-628-7967-9
language
English
LU publication?
yes
id
3d4ba6d6-d924-4052-8aff-dd8e592d79dd (old id 1503117)
date added to LUP
2009-11-17 14:53:27
date last changed
2016-09-19 08:45:17
@misc{3d4ba6d6-d924-4052-8aff-dd8e592d79dd,
  abstract     = {A layer-by-layer deposition technique for assembly of the three-dimensional bioelectrochemical systems, with potential applications such as sensing layers in biosensors or catalytic organisations in biofuel cells, was addressed in this thesis. Several investigations were carried out to understand and control multilayer films formation on planar surfaces, using electrochemical and ellipsometrical methods.<br/><br>
Synthetic and natural/biological polymers were used as building blocks in layer-by-layer films construction. Redox enzymes, such as peroxidases and "blue" multicopper oxidases, were incorporated into multilayer assemblies to provide layers with the catalytic functions. Bioelectrocatalytic activity of the enzymes in the layer structures was evaluated by measuring direct and mediated electron transfer rates between the enzymes and assembly supporting electrodes. In order to enhance direct electron transfer properties of the enzymes in layer-by layer films, gold nanoparticles and carbon nanotubes were used for nanowiring of the redox enzymes.<br/><br>
It was shown that layer-by-layer deposition techniques enable controlled assembly of nanoparticle-enzyme conjugates on the electrode surfaces. This provided a possibility for evaluating kinetic and thermodynamic characteristics of the heterogeneous electron transfer between the redox enzymes and gold nanoparticles.},
  author       = {Haberska, Karolina},
  isbn         = {978-91-628-7967-9},
  keyword      = {layer-by-layer assembly,nanoparticles,electron transfer,bioelectrocatalysis},
  language     = {eng},
  title        = {Protein and polyelectrolyte layer-by-layer films: Assembly and electron transfer},
  year         = {2009},
}