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Increased cysteinyl-leukotrienes and 8-isoprostane in exhaled breath condensate from systemic sclerosis patients.

Tufvesson, Ellen LU ; Bozovic, Gracijela LU ; Hesselstrand, Roger LU ; Bjermer, Leif LU ; Scheja, Agneta LU and Wuttge, Dirk LU (2010) In Rheumatology (Oxford, England) 49. p.2322-2326
Abstract
Objectives. SSc is a systemic CTD characterized by fibrosis in skin and internal organs. Interstitial lung disease is a frequent complication with fibrosis in the lung parenchyma. The fibrotic process is believed to be influenced by leukotrienes (LTs) and also by oxidative stress. The aim of this study was to investigate the amount of LTs and 8-isoprostane, a marker of oxidative stress, in exhaled breath condensate (EBC) from SSc patients. Methods. Twenty-two SSc patients with median disease duration of 2.1 years were investigated. Fifteen patients had lcSSc, four patients had dcSSc and three patients only fulfilled criteria for limited SSc. Sixteen healthy controls were enrolled. Cysteinyl-LTs (CysLTs), LTB(4) and 8-isoprostane were... (More)
Objectives. SSc is a systemic CTD characterized by fibrosis in skin and internal organs. Interstitial lung disease is a frequent complication with fibrosis in the lung parenchyma. The fibrotic process is believed to be influenced by leukotrienes (LTs) and also by oxidative stress. The aim of this study was to investigate the amount of LTs and 8-isoprostane, a marker of oxidative stress, in exhaled breath condensate (EBC) from SSc patients. Methods. Twenty-two SSc patients with median disease duration of 2.1 years were investigated. Fifteen patients had lcSSc, four patients had dcSSc and three patients only fulfilled criteria for limited SSc. Sixteen healthy controls were enrolled. Cysteinyl-LTs (CysLTs), LTB(4) and 8-isoprostane were measured in EBC with EIA and related to the radiologic extent of pulmonary fibrosis. Results. Compared with controls, SSc patients displayed higher median (interquartile range) CysLT [6.1 (5.3-6.8) vs 4.9 (3.7-6.3) pg/ml; P = 0.040], 8-isoprostane [0.23 (0.20-0.46) vs 0.19 (0.12-0.20) pg/ml; P = 0.0020], but similar levels of LTB(4) [0.70 (0.50-0.83) vs 0.60 (0.42-0.70) pg/ml]. CysLT correlated to LTB(4), while 8-isoprostane did not correlate to any of the LTs. None of the biomarkers measured in EBC correlated to radiologic findings. Conclusion. Increased levels of CysLT and 8-isoprostane in EBC from patients with SSc reflect the inflammatory pattern involving LTs as well as oxidative stress. These findings may indicate a possible non-invasive assessment of pulmonary involvement in SSc with a potential value for assessment of disease progress and therapy evaluation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Rheumatology (Oxford, England)
volume
49
pages
2322 - 2326
publisher
Oxford University Press
external identifiers
  • WOS:000284432800014
  • PMID:20702485
  • Scopus:78549293294
ISSN
1462-0332
DOI
10.1093/rheumatology/keq271
language
English
LU publication?
yes
id
c8c4ebef-1c65-4e9b-9d6b-0b75d0289f48 (old id 1665336)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20702485?dopt=Abstract
date added to LUP
2010-09-03 10:52:13
date last changed
2016-10-13 15:12:34
@misc{c8c4ebef-1c65-4e9b-9d6b-0b75d0289f48,
  abstract     = {Objectives. SSc is a systemic CTD characterized by fibrosis in skin and internal organs. Interstitial lung disease is a frequent complication with fibrosis in the lung parenchyma. The fibrotic process is believed to be influenced by leukotrienes (LTs) and also by oxidative stress. The aim of this study was to investigate the amount of LTs and 8-isoprostane, a marker of oxidative stress, in exhaled breath condensate (EBC) from SSc patients. Methods. Twenty-two SSc patients with median disease duration of 2.1 years were investigated. Fifteen patients had lcSSc, four patients had dcSSc and three patients only fulfilled criteria for limited SSc. Sixteen healthy controls were enrolled. Cysteinyl-LTs (CysLTs), LTB(4) and 8-isoprostane were measured in EBC with EIA and related to the radiologic extent of pulmonary fibrosis. Results. Compared with controls, SSc patients displayed higher median (interquartile range) CysLT [6.1 (5.3-6.8) vs 4.9 (3.7-6.3) pg/ml; P = 0.040], 8-isoprostane [0.23 (0.20-0.46) vs 0.19 (0.12-0.20) pg/ml; P = 0.0020], but similar levels of LTB(4) [0.70 (0.50-0.83) vs 0.60 (0.42-0.70) pg/ml]. CysLT correlated to LTB(4), while 8-isoprostane did not correlate to any of the LTs. None of the biomarkers measured in EBC correlated to radiologic findings. Conclusion. Increased levels of CysLT and 8-isoprostane in EBC from patients with SSc reflect the inflammatory pattern involving LTs as well as oxidative stress. These findings may indicate a possible non-invasive assessment of pulmonary involvement in SSc with a potential value for assessment of disease progress and therapy evaluation.},
  author       = {Tufvesson, Ellen and Bozovic, Gracijela and Hesselstrand, Roger and Bjermer, Leif and Scheja, Agneta and Wuttge, Dirk},
  issn         = {1462-0332},
  language     = {eng},
  pages        = {2322--2326},
  publisher    = {ARRAY(0xb867a80)},
  series       = {Rheumatology (Oxford, England)},
  title        = {Increased cysteinyl-leukotrienes and 8-isoprostane in exhaled breath condensate from systemic sclerosis patients.},
  url          = {http://dx.doi.org/10.1093/rheumatology/keq271},
  volume       = {49},
  year         = {2010},
}