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Gene expression analysis in induced sputum from welders with and without airway-related symptoms.

Jönsson, Lena S LU ; Nielsen, Jörn LU and Broberg Palmgren, Karin LU (2011) In International Archives of Occupational and Environmental Health Okt. p.105-113
Abstract
PURPOSE: To identify changes in gene expression in the airways among welders, with and without lower airway symptoms, working in black steel. METHODS: Included were 25 male, non-smoking welders. Each welder was sampled twice; before exposure (after vacation), and after 1 month of exposure. From the welders (14 symptomatic, of whom 7 had asthma-like symptoms), RNA from induced sputum was obtained for gene expression analysis. Messenger RNA from a subset of the samples (n = 7) was analysed with microarray technology to identify genes of interest. These genes were further analysed using quantitative PCR (qPCR; n = 22). RESULTS: By comparing samples before and after exposure, the microarray analysis resulted in several functional annotation... (More)
PURPOSE: To identify changes in gene expression in the airways among welders, with and without lower airway symptoms, working in black steel. METHODS: Included were 25 male, non-smoking welders. Each welder was sampled twice; before exposure (after vacation), and after 1 month of exposure. From the welders (14 symptomatic, of whom 7 had asthma-like symptoms), RNA from induced sputum was obtained for gene expression analysis. Messenger RNA from a subset of the samples (n = 7) was analysed with microarray technology to identify genes of interest. These genes were further analysed using quantitative PCR (qPCR; n = 22). RESULTS: By comparing samples before and after exposure, the microarray analysis resulted in several functional annotation clusters: the one with the highest enrichment score contained "response to wounding", "inflammatory response" and "defence response". Seven genes were analysed by qPCR: granulocyte colony-stimulating factor 3 receptor (CSF3R), superoxide dismutase 2, interleukin 8, glutathione S-transferase pi 1, tumour necrosis factor alpha-induced protein 6 (TNFAIP6), interleukin 1 receptor type II and matrix metallopeptidase 25 (MMP25). Increased levels of CSF3R, TNFAIP6 and MMP25 were indicated among asthmatic subjects compared to non-symptomatic subjects, although the differences did not reach significance. CONCLUSIONS: Workers' exposure to welding fumes changed gene expression in the lower airways in genes involved in inflammatory and defence response. Thus, microarray and qPCR technique can demonstrate markers of exposure to welding fumes and possible disease-related markers. However, further studies are needed to verify genes involved and to further characterise the mechanism for welding fumes-associated lower airway symptoms. (Less)
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organization
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Contribution to journal
publication status
published
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in
International Archives of Occupational and Environmental Health
volume
Okt
pages
105 - 113
publisher
Springer
external identifiers
  • WOS:000286397000011
  • PMID:20862590
  • Scopus:79251481856
ISSN
1432-1246
DOI
10.1007/s00420-010-0579-2
language
English
LU publication?
yes
id
dbf3f273-aba2-4c1e-8fdf-27ea30446b06 (old id 1687961)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20862590?dopt=Abstract
date added to LUP
2010-10-06 08:29:10
date last changed
2016-10-13 04:34:12
@misc{dbf3f273-aba2-4c1e-8fdf-27ea30446b06,
  abstract     = {PURPOSE: To identify changes in gene expression in the airways among welders, with and without lower airway symptoms, working in black steel. METHODS: Included were 25 male, non-smoking welders. Each welder was sampled twice; before exposure (after vacation), and after 1 month of exposure. From the welders (14 symptomatic, of whom 7 had asthma-like symptoms), RNA from induced sputum was obtained for gene expression analysis. Messenger RNA from a subset of the samples (n = 7) was analysed with microarray technology to identify genes of interest. These genes were further analysed using quantitative PCR (qPCR; n = 22). RESULTS: By comparing samples before and after exposure, the microarray analysis resulted in several functional annotation clusters: the one with the highest enrichment score contained "response to wounding", "inflammatory response" and "defence response". Seven genes were analysed by qPCR: granulocyte colony-stimulating factor 3 receptor (CSF3R), superoxide dismutase 2, interleukin 8, glutathione S-transferase pi 1, tumour necrosis factor alpha-induced protein 6 (TNFAIP6), interleukin 1 receptor type II and matrix metallopeptidase 25 (MMP25). Increased levels of CSF3R, TNFAIP6 and MMP25 were indicated among asthmatic subjects compared to non-symptomatic subjects, although the differences did not reach significance. CONCLUSIONS: Workers' exposure to welding fumes changed gene expression in the lower airways in genes involved in inflammatory and defence response. Thus, microarray and qPCR technique can demonstrate markers of exposure to welding fumes and possible disease-related markers. However, further studies are needed to verify genes involved and to further characterise the mechanism for welding fumes-associated lower airway symptoms.},
  author       = {Jönsson, Lena S and Nielsen, Jörn and Broberg Palmgren, Karin},
  issn         = {1432-1246},
  language     = {eng},
  pages        = {105--113},
  publisher    = {ARRAY(0xb4f6e50)},
  series       = {International Archives of Occupational and Environmental Health},
  title        = {Gene expression analysis in induced sputum from welders with and without airway-related symptoms.},
  url          = {http://dx.doi.org/10.1007/s00420-010-0579-2},
  volume       = {Okt},
  year         = {2011},
}