Advanced

Increased β-cell volume in mice fed a high-fat diet: A dynamic study over 12 months.

Ahrén, Jonatan; Ahrén, Bo LU and Wierup, Nils LU (2010) In Islets 2(6). p.12-15
Abstract
As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with β-cell expansion, we have now examined beta volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta cell volume was estimated as β-cell area per islet, individual β-cell size, and β-cell number per islet. Control animals were fed a normal chow (11% fat). We found that β-cell area per islet and total number of beta cells per islet were increased already after three months of high-fat feeding and that this increase was sustained... (More)
As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with β-cell expansion, we have now examined beta volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta cell volume was estimated as β-cell area per islet, individual β-cell size, and β-cell number per islet. Control animals were fed a normal chow (11% fat). We found that β-cell area per islet and total number of beta cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive β-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive β-cell expansion as evident by early increased islet β-cell volume and total number of β-cells, whereas individual β-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta cell volume. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Islets
volume
2
issue
6
pages
12 - 15
publisher
Landes Bioscience
external identifiers
  • PMID:21099337
  • Scopus:78751563884
ISSN
1938-2022
language
English
LU publication?
yes
id
65834e6c-cff6-4c67-a17b-d4f22e6d87fc (old id 1731627)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21099337?dopt=Abstract
date added to LUP
2010-12-01 13:36:02
date last changed
2016-11-27 04:26:06
@misc{65834e6c-cff6-4c67-a17b-d4f22e6d87fc,
  abstract     = {As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with β-cell expansion, we have now examined beta volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta cell volume was estimated as β-cell area per islet, individual β-cell size, and β-cell number per islet. Control animals were fed a normal chow (11% fat). We found that β-cell area per islet and total number of beta cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive β-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive β-cell expansion as evident by early increased islet β-cell volume and total number of β-cells, whereas individual β-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta cell volume.},
  author       = {Ahrén, Jonatan and Ahrén, Bo and Wierup, Nils},
  issn         = {1938-2022},
  language     = {eng},
  number       = {6},
  pages        = {12--15},
  publisher    = {ARRAY(0xa1c4528)},
  series       = {Islets},
  title        = {Increased β-cell volume in mice fed a high-fat diet: A dynamic study over 12 months.},
  volume       = {2},
  year         = {2010},
}