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Reduced iNOS expression in adenoids from children with otitis media with effusion.

Granath, Anna; Norrby-Teglund, Anna; Uddman, Rolf LU and Cardell, Lars-Olaf LU (2010) In Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 21(8). p.1151-1156
Abstract
Granath A, Norrby-Teglund A, Uddman R, Cardell L-O. Reduced iNOS expression in adenoids from children with otitis media with effusion. Pediatr Allergy Immunol 2010: 21: 1151-1156. © 2010 John Wiley & Sons A/S Nitric oxide (NO) is a key mediator in the local immune response of human airways. Inducible NO-synthases (iNOS), and endothelial NO-synthases (eNOS) are two enzymes known to regulate its production. The role of NO in middle ear disease is not fully known. Previous studies suggest that NO might have a dual role, both promoting and suppressing middle ear inflammation. The aim of the present study was to compare the eNOS and iNOS expression in adenoids obtained from children with otitis media with effusion (OME) with the expression... (More)
Granath A, Norrby-Teglund A, Uddman R, Cardell L-O. Reduced iNOS expression in adenoids from children with otitis media with effusion. Pediatr Allergy Immunol 2010: 21: 1151-1156. © 2010 John Wiley & Sons A/S Nitric oxide (NO) is a key mediator in the local immune response of human airways. Inducible NO-synthases (iNOS), and endothelial NO-synthases (eNOS) are two enzymes known to regulate its production. The role of NO in middle ear disease is not fully known. Previous studies suggest that NO might have a dual role, both promoting and suppressing middle ear inflammation. The aim of the present study was to compare the eNOS and iNOS expression in adenoids obtained from children with otitis media with effusion (OME) with the expression seen in adenoids derived from children without middle ear disease. In addition, the expression of IL-1β and TNF-α were analyzed, because of their role in the iNOS-induction pathway. The iNOS and eNOS expression were analyzed with real-time PCR in 8 OME and 11 control adenoids. The corresponding proteins were demonstrated by immunohistochemical staining of adenoid tissue. A Luminex(®) assay was performed to analyze IL-1β and TNF-α in nasopharyngeal secretion in 10 OME and 8 controls, and immunohistochemistry was performed on adenoid tissue and imprints from the adenoid surface. Children with OME exhibited lower levels of iNOS than controls without middle ear disease. No such difference was seen for eNOS. The corresponding proteins were found mainly in conjunction with surface epithelium. No significant changes were seen among the cytokines tested. The present results indicate that local induction of iNOS in adenoids might be of importance for preventing development of OME. (Less)
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Contribution to journal
publication status
published
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in
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology
volume
21
issue
8
pages
1151 - 1156
external identifiers
  • WOS:000284279100008
  • PMID:21073541
  • Scopus:78449250776
ISSN
1399-3038
DOI
10.1111/j.1399-3038.2010.01020.x
language
English
LU publication?
yes
id
66ec8a4c-a808-4a77-8d3c-4b575bf429ba (old id 1732057)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21073541?dopt=Abstract
date added to LUP
2010-12-01 11:39:05
date last changed
2016-10-13 04:24:39
@misc{66ec8a4c-a808-4a77-8d3c-4b575bf429ba,
  abstract     = {Granath A, Norrby-Teglund A, Uddman R, Cardell L-O. Reduced iNOS expression in adenoids from children with otitis media with effusion. Pediatr Allergy Immunol 2010: 21: 1151-1156. © 2010 John Wiley & Sons A/S Nitric oxide (NO) is a key mediator in the local immune response of human airways. Inducible NO-synthases (iNOS), and endothelial NO-synthases (eNOS) are two enzymes known to regulate its production. The role of NO in middle ear disease is not fully known. Previous studies suggest that NO might have a dual role, both promoting and suppressing middle ear inflammation. The aim of the present study was to compare the eNOS and iNOS expression in adenoids obtained from children with otitis media with effusion (OME) with the expression seen in adenoids derived from children without middle ear disease. In addition, the expression of IL-1β and TNF-α were analyzed, because of their role in the iNOS-induction pathway. The iNOS and eNOS expression were analyzed with real-time PCR in 8 OME and 11 control adenoids. The corresponding proteins were demonstrated by immunohistochemical staining of adenoid tissue. A Luminex(®) assay was performed to analyze IL-1β and TNF-α in nasopharyngeal secretion in 10 OME and 8 controls, and immunohistochemistry was performed on adenoid tissue and imprints from the adenoid surface. Children with OME exhibited lower levels of iNOS than controls without middle ear disease. No such difference was seen for eNOS. The corresponding proteins were found mainly in conjunction with surface epithelium. No significant changes were seen among the cytokines tested. The present results indicate that local induction of iNOS in adenoids might be of importance for preventing development of OME.},
  author       = {Granath, Anna and Norrby-Teglund, Anna and Uddman, Rolf and Cardell, Lars-Olaf},
  issn         = {1399-3038},
  language     = {eng},
  number       = {8},
  pages        = {1151--1156},
  series       = {Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology},
  title        = {Reduced iNOS expression in adenoids from children with otitis media with effusion.},
  url          = {http://dx.doi.org/10.1111/j.1399-3038.2010.01020.x},
  volume       = {21},
  year         = {2010},
}