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Phosphatidylcholine enhances the activity of rat liver type II phosphatidylinositol-kinase

Olsson, H; Martinez Arias, Wilma LU and Jergil, Bengt LU (1993) In FEBS Lett. 327(3). p.332-6
Abstract
A PtdIns 4-kinase was purified extensively from rat liver exocytotic vesicles. The enzyme had a low Km for ATP, was inhibited by adenosine, and had an apparent molecular mass of 54 kDa, indicating it to be a type II PtdIns-kinase. The activity of the purified enzyme was enhanced several-fold by PtdCho, and to some extent by other phospholipids with basic polar head groups, and was inhibited by PtdSer. Kinetic analyses, presenting the substrate in mixed micelles of Triton X-100, PtdIns and PtdCho, showed that the effect of PtdCho was both to increase Vmax and to decrease the apparent Km for micellar PtdIns.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gel Enzyme Activation Exocytosis Liver/enzymology* Micelles Phosphatidylcholines/pharmacology* Phospholipids/pharmacology Phosphotransferases/isolation & purification Phosphotransferases/metabolism* Rats, 1-Phosphatidylinositol 4-Kinase Animals Chromatography
in
FEBS Lett.
volume
327
issue
3
pages
332 - 6
external identifiers
  • Scopus:0027257154
DOI
10.1016/0014-5793(93)81015-R
language
English
LU publication?
yes
id
9edd2356-3fdc-4e30-8a43-c08e8564ef28 (old id 1753801)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/8394247
date added to LUP
2010-12-29 11:05:56
date last changed
2016-04-16 11:33:25
@misc{9edd2356-3fdc-4e30-8a43-c08e8564ef28,
  abstract     = {A PtdIns 4-kinase was purified extensively from rat liver exocytotic vesicles. The enzyme had a low Km for ATP, was inhibited by adenosine, and had an apparent molecular mass of 54 kDa, indicating it to be a type II PtdIns-kinase. The activity of the purified enzyme was enhanced several-fold by PtdCho, and to some extent by other phospholipids with basic polar head groups, and was inhibited by PtdSer. Kinetic analyses, presenting the substrate in mixed micelles of Triton X-100, PtdIns and PtdCho, showed that the effect of PtdCho was both to increase Vmax and to decrease the apparent Km for micellar PtdIns.},
  author       = {Olsson, H and Martinez Arias, Wilma and Jergil, Bengt},
  keyword      = {Gel
Enzyme Activation
Exocytosis
Liver/enzymology*
Micelles
Phosphatidylcholines/pharmacology*
Phospholipids/pharmacology
Phosphotransferases/isolation & purification
Phosphotransferases/metabolism*
Rats,1-Phosphatidylinositol 4-Kinase
Animals
Chromatography},
  language     = {eng},
  number       = {3},
  pages        = {332--6},
  series       = {FEBS Lett.},
  title        = {Phosphatidylcholine enhances the activity of rat liver type II phosphatidylinositol-kinase},
  url          = {http://dx.doi.org/10.1016/0014-5793(93)81015-R},
  volume       = {327},
  year         = {1993},
}