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Conserved function of the Krüppel gap gene in the blowfly Lucilia sericata, despite anterior shift of expression.

Blechert, Oliver; Douglas, Desiree LU and Baumgartner, Stefan LU (2011) In Insect Molecular Biology 20. p.257-265
Abstract
To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an... (More)
To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an anterior shift in expression, Kr function has remained conserved during the evolution of the blowflies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Insect Molecular Biology
volume
20
pages
257 - 265
publisher
Wiley-Blackwell
external identifiers
  • WOS:000287927400010
  • PMID:21166911
  • Scopus:79952300556
ISSN
1365-2583
DOI
10.1111/j.1365-2583.2010.01063.x
language
English
LU publication?
yes
id
5af33f0f-e3a7-4427-b201-910e559d9537 (old id 1756223)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21166911?dopt=Abstract
date added to LUP
2011-01-03 15:55:58
date last changed
2016-11-06 04:28:15
@misc{5af33f0f-e3a7-4427-b201-910e559d9537,
  abstract     = {To determine whether expression patterns of segmentation genes found in Drosophila melanogaster can be scaled to pattern larger insects, we studied the expression of the Krüppel (Kr) gene in the blowfly Lucilia sericata. Compared with Drosophila Kr, L. sericata Kr showed an unexpected 10% shift of expression towards the anterior pole. Furthermore, expression domains not found in D. melanogaster were present at the blastoderm stage of L. sericata. To compare Kr activity and function, we employed RNA interference-mediated gene silencing. We found Kr phenotypes in L. sericata comparable with those observed in D. melanogaster, demonstrating that L. sericata Kr functions as a gap gene as it does in Drosophila. Our results show that, despite an anterior shift in expression, Kr function has remained conserved during the evolution of the blowflies.},
  author       = {Blechert, Oliver and Douglas, Desiree and Baumgartner, Stefan},
  issn         = {1365-2583},
  language     = {eng},
  pages        = {257--265},
  publisher    = {ARRAY(0x9e52be0)},
  series       = {Insect Molecular Biology},
  title        = {Conserved function of the Krüppel gap gene in the blowfly Lucilia sericata, despite anterior shift of expression.},
  url          = {http://dx.doi.org/10.1111/j.1365-2583.2010.01063.x},
  volume       = {20},
  year         = {2011},
}