Differential regulation of alpha and beta chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S
(1994) In Blood 84(3). p.815-822- Abstract
Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase... (More)
Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BP beta+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP alpha- and beta-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BP beta+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.
(Less)
- author
- Garcia de Frutos, Pablo LU ; Alim, R ; Härdig, Ylva ; Zöller, Bengt LU and Dahlbäck, Björn LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acute-Phase Proteins, Acute-Phase Reaction, Antibodies, Monoclonal, Calcium, Carrier Proteins, Complement C4b, Complement Inactivator Proteins, Glycoproteins, Humans, Macromolecular Substances, Protein S, Journal Article, Research Support, Non-U.S. Gov't
- in
- Blood
- volume
- 84
- issue
- 3
- pages
- 8 pages
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:7519078
- scopus:0028103281
- ISSN
- 1528-0020
- language
- English
- LU publication?
- yes
- id
- 8bad4052-e453-4a54-870c-4dccca3199f4 (old id 1894793)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/7519078
- http://bloodjournal.hematologylibrary.org/content/84/3/815.long
- date added to LUP
- 2016-04-04 13:36:06
- date last changed
- 2021-03-21 04:40:47
@article{8bad4052-e453-4a54-870c-4dccca3199f4, abstract = {{<p>Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BP beta+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP alpha- and beta-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BP beta+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.</p>}}, author = {{Garcia de Frutos, Pablo and Alim, R and Härdig, Ylva and Zöller, Bengt and Dahlbäck, Björn}}, issn = {{1528-0020}}, keywords = {{Acute-Phase Proteins; Acute-Phase Reaction; Antibodies, Monoclonal; Calcium; Carrier Proteins; Complement C4b; Complement Inactivator Proteins; Glycoproteins; Humans; Macromolecular Substances; Protein S; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, number = {{3}}, pages = {{815--822}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{Differential regulation of alpha and beta chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S}}, url = {{http://www.ncbi.nlm.nih.gov/pubmed/7519078}}, volume = {{84}}, year = {{1994}}, }