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C-type Lectin Receptor Expression on Human Basophils and Effects of Allergen-Specific Immunotherapy

Lundberg, K. LU ; Rydnert, F. LU ; Broos, S. LU ; Andersson, Martin; Greiff, L. LU and Lindstedt, M. LU (2016) In Scandinavian Journal of Immunology 84(3). p.150-157
Abstract

Basophils are emerging as immunoregulatory cells capable of interacting with their environment not only via their characteristic IgE-mediated activation, but also in an IgE-independent manner. Basophils are known to express and respond to stimulation via TLR2, TLR4, DC-SIGN and DCIR, but whether basophils also express other C-type lectin receptors (CLRs) is largely unknown. In this study, we investigate the CLR expression profile of human basophils using multicolour flow cytometry. As FcRs as well as some CLRs are associated with allergen recognition and shown to be involved in subsequent immune responses, the expression of CLRs and FcRs on peripheral blood basophils, as well as their frequency, was monitored for 1 year in subjects... (More)

Basophils are emerging as immunoregulatory cells capable of interacting with their environment not only via their characteristic IgE-mediated activation, but also in an IgE-independent manner. Basophils are known to express and respond to stimulation via TLR2, TLR4, DC-SIGN and DCIR, but whether basophils also express other C-type lectin receptors (CLRs) is largely unknown. In this study, we investigate the CLR expression profile of human basophils using multicolour flow cytometry. As FcRs as well as some CLRs are associated with allergen recognition and shown to be involved in subsequent immune responses, the expression of CLRs and FcRs on peripheral blood basophils, as well as their frequency, was monitored for 1 year in subjects undergoing subcutaneous allergen-specific immunotherapy (AIT). Here, we show that human basophils express CLECSF14, DEC205, Dectin-1, Dectin-2 and MRC2. Furthermore, we demonstrate that the frequencies of basophils expressing the allergy-associated CLRs Dectin-1 and Dectin-2 were significantly reduced after 1 year and 8 weeks of AIT, respectively. In contrast, the frequency of basophils positive for FcγRII, as well as the fraction of total basophils, significantly increased after 1 year of AIT. The herein demonstrated expression of various CLRs on basophils, and their altered CLR and FcR expression profile upon AIT, suggest yet unexplored ways by which basophils can interact with antigens and may point to novel immunoregulatory functions targeted through AIT.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Immunology
volume
84
issue
3
pages
8 pages
publisher
Wiley-Blackwell
external identifiers
  • Scopus:84983657260
ISSN
0300-9475
DOI
10.1111/sji.12457
language
English
LU publication?
yes
id
1a7977ee-d437-4674-ba00-9cac71d86dde
date added to LUP
2016-09-28 13:38:37
date last changed
2016-09-28 16:34:09
@misc{1a7977ee-d437-4674-ba00-9cac71d86dde,
  abstract     = {<p>Basophils are emerging as immunoregulatory cells capable of interacting with their environment not only via their characteristic IgE-mediated activation, but also in an IgE-independent manner. Basophils are known to express and respond to stimulation via TLR2, TLR4, DC-SIGN and DCIR, but whether basophils also express other C-type lectin receptors (CLRs) is largely unknown. In this study, we investigate the CLR expression profile of human basophils using multicolour flow cytometry. As FcRs as well as some CLRs are associated with allergen recognition and shown to be involved in subsequent immune responses, the expression of CLRs and FcRs on peripheral blood basophils, as well as their frequency, was monitored for 1 year in subjects undergoing subcutaneous allergen-specific immunotherapy (AIT). Here, we show that human basophils express CLECSF14, DEC205, Dectin-1, Dectin-2 and MRC2. Furthermore, we demonstrate that the frequencies of basophils expressing the allergy-associated CLRs Dectin-1 and Dectin-2 were significantly reduced after 1 year and 8 weeks of AIT, respectively. In contrast, the frequency of basophils positive for FcγRII, as well as the fraction of total basophils, significantly increased after 1 year of AIT. The herein demonstrated expression of various CLRs on basophils, and their altered CLR and FcR expression profile upon AIT, suggest yet unexplored ways by which basophils can interact with antigens and may point to novel immunoregulatory functions targeted through AIT.</p>},
  author       = {Lundberg, K. and Rydnert, F. and Broos, S. and Andersson, Martin and Greiff, L. and Lindstedt, M.},
  issn         = {0300-9475},
  language     = {eng},
  month        = {09},
  number       = {3},
  pages        = {150--157},
  publisher    = {ARRAY(0x9b5df30)},
  series       = {Scandinavian Journal of Immunology},
  title        = {C-type Lectin Receptor Expression on Human Basophils and Effects of Allergen-Specific Immunotherapy},
  url          = {http://dx.doi.org/10.1111/sji.12457},
  volume       = {84},
  year         = {2016},
}