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Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework

Jirholt, P. LU ; Ohlin, M. LU ; Borrebaeck, C. A K LU and Söderlind, E. LU (1998) In Gene 215(2). p.471-476
Abstract

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
CDR shuffling, Gene assembly, Immunoglobulin germline gene, Primer design, Synthetic gene library
in
Gene
volume
215
issue
2
pages
6 pages
publisher
Elsevier
external identifiers
  • Scopus:0032581610
ISSN
0378-1119
DOI
10.1016/S0378-1119(98)00317-5
language
English
LU publication?
yes
id
1f8420e5-3eb1-4692-a63b-a63698c8fa86
date added to LUP
2016-04-19 14:06:37
date last changed
2016-05-16 14:49:18
@misc{1f8420e5-3eb1-4692-a63b-a63698c8fa86,
  abstract     = {<p>A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.</p>},
  author       = {Jirholt, P. and Ohlin, M. and Borrebaeck, C. A K and Söderlind, E.},
  issn         = {0378-1119},
  keyword      = {CDR shuffling,Gene assembly,Immunoglobulin germline gene,Primer design,Synthetic gene library},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {471--476},
  publisher    = {ARRAY(0xa07bf78)},
  series       = {Gene},
  title        = {Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework},
  url          = {http://dx.doi.org/10.1016/S0378-1119(98)00317-5},
  volume       = {215},
  year         = {1998},
}