Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework
(1998) In Gene 215(2). p.471-476- Abstract
A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1f8420e5-3eb1-4692-a63b-a63698c8fa86
- author
- Jirholt, P. LU ; Ohlin, M. LU ; Borrebaeck, C. A K LU and Söderlind, E. LU
- organization
- publishing date
- 1998-07-30
- type
- Contribution to journal
- publication status
- published
- keywords
- CDR shuffling, Gene assembly, Immunoglobulin germline gene, Primer design, Synthetic gene library
- in
- Gene
- volume
- 215
- issue
- 2
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0032581610
- pmid:9714846
- ISSN
- 0378-1119
- DOI
- 10.1016/S0378-1119(98)00317-5
- language
- English
- LU publication?
- yes
- id
- 1f8420e5-3eb1-4692-a63b-a63698c8fa86
- date added to LUP
- 2016-04-19 14:06:37
- date last changed
- 2024-08-23 08:30:34
@article{1f8420e5-3eb1-4692-a63b-a63698c8fa86, abstract = {{<p>A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.</p>}}, author = {{Jirholt, P. and Ohlin, M. and Borrebaeck, C. A K and Söderlind, E.}}, issn = {{0378-1119}}, keywords = {{CDR shuffling; Gene assembly; Immunoglobulin germline gene; Primer design; Synthetic gene library}}, language = {{eng}}, month = {{07}}, number = {{2}}, pages = {{471--476}}, publisher = {{Elsevier}}, series = {{Gene}}, title = {{Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework}}, url = {{http://dx.doi.org/10.1016/S0378-1119(98)00317-5}}, doi = {{10.1016/S0378-1119(98)00317-5}}, volume = {{215}}, year = {{1998}}, }