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Crystallization and preliminary X-ray investigation of a recombinant form of rat catechol O-methyltransferase

Vidgren, J.; Tilgmann, C. LU ; Lundstrom, K. and Liljas, A. LU (1991) In Proteins 11(3). p.233-236
Abstract

Rat catechol O-methyltransferase cDNA was introduced into an E. coli expression vector pKEX14, which utilizes the inducible T7 promoter. Active and soluble recombinant catechol O-methyltransferase was produced in bacteria and purified to electrophoretic homogeneity by chromatographic procedures. The purified enzyme has been crystallized by the method of vapor diffusion using polyethylene glycol as precipitant. The space group is P3121 or P3221 with a = b = 51.3 Å and c = 168.5 Å and one molecule in the asymmetric unit. The crystals diffract beyond 3.2 Å and are suitable for three-dimensional X-ray structure determination.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
catechol O-methyltransferase, protein crystals, protein structure, X-ray crystallography
in
Proteins
volume
11
issue
3
pages
4 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0025936299
ISSN
0887-3585
language
English
LU publication?
no
id
21233a13-4320-41dd-b288-ae5419a160e7
date added to LUP
2016-04-11 13:25:28
date last changed
2016-11-27 04:39:17
@misc{21233a13-4320-41dd-b288-ae5419a160e7,
  abstract     = {<p>Rat catechol O-methyltransferase cDNA was introduced into an E. coli expression vector pKEX14, which utilizes the inducible T7 promoter. Active and soluble recombinant catechol O-methyltransferase was produced in bacteria and purified to electrophoretic homogeneity by chromatographic procedures. The purified enzyme has been crystallized by the method of vapor diffusion using polyethylene glycol as precipitant. The space group is P3<sub>1</sub>21 or P3<sub>2</sub>21 with a = b = 51.3 Å and c = 168.5 Å and one molecule in the asymmetric unit. The crystals diffract beyond 3.2 Å and are suitable for three-dimensional X-ray structure determination.</p>},
  author       = {Vidgren, J. and Tilgmann, C. and Lundstrom, K. and Liljas, A.},
  issn         = {0887-3585},
  keyword      = {catechol O-methyltransferase,protein crystals,protein structure,X-ray crystallography},
  language     = {eng},
  number       = {3},
  pages        = {233--236},
  publisher    = {ARRAY(0xb8fbe70)},
  series       = {Proteins},
  title        = {Crystallization and preliminary X-ray investigation of a recombinant form of rat catechol O-methyltransferase},
  volume       = {11},
  year         = {1991},
}