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Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function-spacer-lipid A and B constructs.

Hult, Annika LU ; Frame, Tom; Chesla, Scott; Henry, Stephen and Olsson, Martin L LU (2012) In Transfusion 52. p.247-251
Abstract
BACKGROUND:

Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A(weak) and B(weak) subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.



STUDY DESIGN AND METHODS:

A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function-spacer-lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of... (More)
BACKGROUND:

Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A(weak) and B(weak) subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.



STUDY DESIGN AND METHODS:

A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function-spacer-lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of native subgroup cells.



RESULTS:

Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2 µg/mL FSL-A or 78 to 10 µg/mL FSL-B transformation solutions. The kodecytes created with higher concentrations of FSL constructs revealed a uniform and/or even distribution of antigens as seen by a single flow cytometry peak more narrow than the broader peaks produced with lower FSL concentrations similar to those found in native A(x) and most B(weak) subgroups.



CONCLUSIONS:

Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transfusion
volume
52
pages
247 - 251
publisher
Wiley-Blackwell
external identifiers
  • WOS:000299038200009
  • PMID:21812783
  • Scopus:84855851546
ISSN
1537-2995
DOI
10.1111/j.1537-2995.2011.03268.x
language
English
LU publication?
yes
id
2d60fa8b-9f93-454e-b224-231e175b8e10 (old id 2151561)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21812783?dopt=Abstract
date added to LUP
2011-09-04 21:16:01
date last changed
2016-10-30 04:36:26
@misc{2d60fa8b-9f93-454e-b224-231e175b8e10,
  abstract     = {BACKGROUND:<br/><br>
Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) A(weak) and B(weak) subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.<br/><br>
<br/><br>
STUDY DESIGN AND METHODS:<br/><br>
A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function-spacer-lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of native subgroup cells.<br/><br>
<br/><br>
RESULTS:<br/><br>
Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2 µg/mL FSL-A or 78 to 10 µg/mL FSL-B transformation solutions. The kodecytes created with higher concentrations of FSL constructs revealed a uniform and/or even distribution of antigens as seen by a single flow cytometry peak more narrow than the broader peaks produced with lower FSL concentrations similar to those found in native A(x) and most B(weak) subgroups.<br/><br>
<br/><br>
CONCLUSIONS:<br/><br>
Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs.},
  author       = {Hult, Annika and Frame, Tom and Chesla, Scott and Henry, Stephen and Olsson, Martin L},
  issn         = {1537-2995},
  language     = {eng},
  pages        = {247--251},
  publisher    = {ARRAY(0xb8c1520)},
  series       = {Transfusion},
  title        = {Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function-spacer-lipid A and B constructs.},
  url          = {http://dx.doi.org/10.1111/j.1537-2995.2011.03268.x},
  volume       = {52},
  year         = {2012},
}