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A ranking index for quality assessment of forensic DNA profiles

Hedman, Johannes LU ; Ansell, Ricky and Nordgaard, Anders (2010) In BMC Research Notes 3.
Abstract
Background: Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the

evidentiary value of DNA results and to compare the performance of different DNA analysis protocols. Generally

the quality assessment is performed through manual examination of the DNA profiles based on empirical

knowledge, or by comparing the intensities (allelic peak heights) of the capillary electrophoresis electropherograms.

Results: We recently developed a ranking index for unbiased and quantitative quality assessment of forensic DNA

profiles, the forensic DNA profile index (FI) (Hedman et al. Improved forensic DNA analysis through the use of

alternative DNA polymerases... (More)
Background: Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the

evidentiary value of DNA results and to compare the performance of different DNA analysis protocols. Generally

the quality assessment is performed through manual examination of the DNA profiles based on empirical

knowledge, or by comparing the intensities (allelic peak heights) of the capillary electrophoresis electropherograms.

Results: We recently developed a ranking index for unbiased and quantitative quality assessment of forensic DNA

profiles, the forensic DNA profile index (FI) (Hedman et al. Improved forensic DNA analysis through the use of

alternative DNA polymerases and statistical modeling of DNA profiles, Biotechniques 47 (2009) 951-958). FI uses

electropherogram data to combine the intensities of the allelic peaks with the balances within and between loci,

using Principal Components Analysis. Here we present the construction of FI. We explain the mathematical and

statistical methodologies used and present details about the applied data reduction method. Thereby we show

how to adapt the ranking index for any Short Tandem Repeat-based forensic DNA typing system through

validation against a manual grading scale and calibration against a specific set of DNA profiles.

Conclusions: The developed tool provides unbiased quality assessment of forensic DNA profiles. It can be applied

for any DNA profiling system based on Short Tandem Repeat markers. Apart from crime related DNA analysis, FI

can therefore be used as a quality tool in paternal or familial testing as well as in disaster victim identification. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Forensic DNA analysis, quality assessment, DNA profile, mathematical modelling
in
BMC Research Notes
volume
3
publisher
BioMed Central
external identifiers
  • Scopus:78049500787
ISSN
1756-0500
DOI
10.1186/1756-0500-3-290
language
English
LU publication?
yes
id
79a892c4-5043-47b0-bdb4-da06d5f9f3ec (old id 2157820)
date added to LUP
2011-09-20 15:54:48
date last changed
2016-10-13 04:40:06
@misc{79a892c4-5043-47b0-bdb4-da06d5f9f3ec,
  abstract     = {Background: Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the<br/><br>
evidentiary value of DNA results and to compare the performance of different DNA analysis protocols. Generally<br/><br>
the quality assessment is performed through manual examination of the DNA profiles based on empirical<br/><br>
knowledge, or by comparing the intensities (allelic peak heights) of the capillary electrophoresis electropherograms.<br/><br>
Results: We recently developed a ranking index for unbiased and quantitative quality assessment of forensic DNA<br/><br>
profiles, the forensic DNA profile index (FI) (Hedman et al. Improved forensic DNA analysis through the use of<br/><br>
alternative DNA polymerases and statistical modeling of DNA profiles, Biotechniques 47 (2009) 951-958). FI uses<br/><br>
electropherogram data to combine the intensities of the allelic peaks with the balances within and between loci,<br/><br>
using Principal Components Analysis. Here we present the construction of FI. We explain the mathematical and<br/><br>
statistical methodologies used and present details about the applied data reduction method. Thereby we show<br/><br>
how to adapt the ranking index for any Short Tandem Repeat-based forensic DNA typing system through<br/><br>
validation against a manual grading scale and calibration against a specific set of DNA profiles.<br/><br>
Conclusions: The developed tool provides unbiased quality assessment of forensic DNA profiles. It can be applied<br/><br>
for any DNA profiling system based on Short Tandem Repeat markers. Apart from crime related DNA analysis, FI<br/><br>
can therefore be used as a quality tool in paternal or familial testing as well as in disaster victim identification.},
  author       = {Hedman, Johannes and Ansell, Ricky and Nordgaard, Anders},
  issn         = {1756-0500},
  keyword      = {Forensic DNA analysis,quality assessment,DNA profile,mathematical modelling},
  language     = {eng},
  publisher    = {ARRAY(0xba9ac80)},
  series       = {BMC Research Notes},
  title        = {A ranking index for quality assessment of forensic DNA profiles},
  url          = {http://dx.doi.org/10.1186/1756-0500-3-290},
  volume       = {3},
  year         = {2010},
}