A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus
(2011) In Biotechnology for Biofuels 4:31(1). p.1-15- Abstract
- Background: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2)producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H2 and osmotic pressure, as well as the liquid-to-gas mass transfer of H2.
Results: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were... (More) - Background: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2)producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H2 and osmotic pressure, as well as the liquid-to-gas mass transfer of H2.
Results: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were varied. The model was used to simulate the inhibition of growth
by H2 and solute concentrations, giving a critical dissolved H2 concentration of 2.2 mmol/L and an osmolarity of 0.27 to 29 mol/L. The inhibition by H2, being a function of the dissolved H2 concentration, was demonstrated to be mainly dependent on H2 productivity and mass transfer rate. The latter can be improved by increasing the stripping rate, thereby allowing higher H2 productivity. The experimentally determined degree of oversaturation of dissolved H2 was 12 to 34 times the equilibrium concentration and was comparable to the values given by the model.
Conclusions: The derived model is the first mechanistically based model for fermentative H2 production and provides useful information to improve the understanding of the growth behavior of C. saccharolyticus. The model
can be used to determine optimal operating conditions for H2 production regarding the substrate concentration and the stripping rate. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2199498
- author
- Ljunggren, Mattias LU ; Willquist, Karin LU ; Zacchi, Guido LU and van Niel, Ed LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Caldicellulosiruptor saccharolyticus kinetic growth model hydrogen production osmolarity
- in
- Biotechnology for Biofuels
- volume
- 4:31
- issue
- 1
- pages
- 1 - 15
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000295872700001
- scopus:80052665545
- ISSN
- 1754-6834
- DOI
- 10.1186/1754-6834-4-31
- language
- English
- LU publication?
- yes
- id
- b3380d0a-b6eb-4fc0-a015-8cb1ccf8cf73 (old id 2199498)
- date added to LUP
- 2016-04-04 12:01:09
- date last changed
- 2023-11-16 05:08:05
@article{b3380d0a-b6eb-4fc0-a015-8cb1ccf8cf73,
abstract = {{Background: Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2)producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The model is based on Monod kinetics supplemented with the inhibitory effects of H2 and osmotic pressure, as well as the liquid-to-gas mass transfer of H2.<br/><br>
Results: Mathematical expressions were developed to enable the simulation of microbial growth, substrate consumption and product formation. The model parameters were determined by fitting them to experimental data. The derived model corresponded well with experimental data from batch fermentations in which the stripping rates and substrate concentrations were varied. The model was used to simulate the inhibition of growth<br/><br>
by H2 and solute concentrations, giving a critical dissolved H2 concentration of 2.2 mmol/L and an osmolarity of 0.27 to 29 mol/L. The inhibition by H2, being a function of the dissolved H2 concentration, was demonstrated to be mainly dependent on H2 productivity and mass transfer rate. The latter can be improved by increasing the stripping rate, thereby allowing higher H2 productivity. The experimentally determined degree of oversaturation of dissolved H2 was 12 to 34 times the equilibrium concentration and was comparable to the values given by the model.<br/><br>
Conclusions: The derived model is the first mechanistically based model for fermentative H2 production and provides useful information to improve the understanding of the growth behavior of C. saccharolyticus. The model<br/><br>
can be used to determine optimal operating conditions for H2 production regarding the substrate concentration and the stripping rate.}},
author = {{Ljunggren, Mattias and Willquist, Karin and Zacchi, Guido and van Niel, Ed}},
issn = {{1754-6834}},
keywords = {{Caldicellulosiruptor saccharolyticus kinetic growth model hydrogen production osmolarity}},
language = {{eng}},
number = {{1}},
pages = {{1--15}},
publisher = {{BioMed Central (BMC)}},
series = {{Biotechnology for Biofuels}},
title = {{A kinetic model for quantitative evaluation of the effect of hydrogen and osmolarity on hydrogen production by Caldicellulosiruptor saccharolyticus}},
url = {{http://dx.doi.org/10.1186/1754-6834-4-31}},
doi = {{10.1186/1754-6834-4-31}},
volume = {{4:31}},
year = {{2011}},
}