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Adipose-derived Stem Cells Counteract Urethral Stricture Formation in Rats

Castiglione, Fabio; Dewulf, Karel; Hakim, Lukman; Weyne, Emanuel; Montorsi, Francesco; Russo, Andrea; Boeri, Luca; Bivalacqua, Trinity J.; De Ridder, Dirk and Joniau, Steven, et al. (2016) In European Urology
Abstract

Background: A medical treatment for urethral stricture (US) is not yet available. Objective: To evaluate if local injection of human adipose tissue-derived stem cells (hADSC) prevents urethral fibrosis in a rat model of US. Design, setting, and participants: Male rats were divided into three groups: sham, US, and hADSC (n = 12 each). Sham rats received a vehicle injection in the urethral wall. US and hADSCs were incised and injected with the fibrosis-inducer transforming growth factor-β1 in the urethral wall. Intervention: One day later, hADSCs were injected in the urethral wall of hADSC rats whereas sham and US rats were injected with the vehicle. After 4 wk, the rats underwent cystometries and tissues were then harvested for... (More)

Background: A medical treatment for urethral stricture (US) is not yet available. Objective: To evaluate if local injection of human adipose tissue-derived stem cells (hADSC) prevents urethral fibrosis in a rat model of US. Design, setting, and participants: Male rats were divided into three groups: sham, US, and hADSC (n = 12 each). Sham rats received a vehicle injection in the urethral wall. US and hADSCs were incised and injected with the fibrosis-inducer transforming growth factor-β1 in the urethral wall. Intervention: One day later, hADSCs were injected in the urethral wall of hADSC rats whereas sham and US rats were injected with the vehicle. After 4 wk, the rats underwent cystometries and tissues were then harvested for functional and molecular analyses. Outcome measurements and statistical analysis: Cystometry, microultrasound, histochemistry, organ bath studies, reverse transcription polymerase chain reaction, and western blot. Results and limitations: US rats exhibited 49-51% shorter micturition intervals, 35-51% smaller micturition volumes and bladder capacity, 33-62% higher threshold pressures and flow pressures, and 35-37% lower bladder filling compliance compared with hADSC-treated rats and sham rats (p

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Contribution to journal
publication status
in press
subject
keywords
Animal model, Stem cell, Therapy, Urethral stricture
in
European Urology
publisher
Elsevier
external identifiers
  • Scopus:84964734804
ISSN
0302-2838
DOI
10.1016/j.eururo.2016.04.022
language
English
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yes
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219a51e7-066d-4efd-87cb-1ac202362a7b
date added to LUP
2016-06-01 13:03:59
date last changed
2016-11-14 13:44:39
@misc{219a51e7-066d-4efd-87cb-1ac202362a7b,
  abstract     = {<p>Background: A medical treatment for urethral stricture (US) is not yet available. Objective: To evaluate if local injection of human adipose tissue-derived stem cells (hADSC) prevents urethral fibrosis in a rat model of US. Design, setting, and participants: Male rats were divided into three groups: sham, US, and hADSC (n = 12 each). Sham rats received a vehicle injection in the urethral wall. US and hADSCs were incised and injected with the fibrosis-inducer transforming growth factor-β1 in the urethral wall. Intervention: One day later, hADSCs were injected in the urethral wall of hADSC rats whereas sham and US rats were injected with the vehicle. After 4 wk, the rats underwent cystometries and tissues were then harvested for functional and molecular analyses. Outcome measurements and statistical analysis: Cystometry, microultrasound, histochemistry, organ bath studies, reverse transcription polymerase chain reaction, and western blot. Results and limitations: US rats exhibited 49-51% shorter micturition intervals, 35-51% smaller micturition volumes and bladder capacity, 33-62% higher threshold pressures and flow pressures, and 35-37% lower bladder filling compliance compared with hADSC-treated rats and sham rats (p </p>},
  author       = {Castiglione, Fabio and Dewulf, Karel and Hakim, Lukman and Weyne, Emanuel and Montorsi, Francesco and Russo, Andrea and Boeri, Luca and Bivalacqua, Trinity J. and De Ridder, Dirk and Joniau, Steven and Albersen, Maarten and Hedlund, Petter},
  issn         = {0302-2838},
  keyword      = {Animal model,Stem cell,Therapy,Urethral stricture},
  language     = {eng},
  publisher    = {ARRAY(0x8ce4878)},
  series       = {European Urology},
  title        = {Adipose-derived Stem Cells Counteract Urethral Stricture Formation in Rats},
  url          = {http://dx.doi.org/10.1016/j.eururo.2016.04.022},
  year         = {2016},
}