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PIXE Analysis of Blood Serum Proteins, Separated by Gel Filtration

Pallon, Jan LU ; Pakarinen, Pirjo and Akselsson, Roland LU (1984) In Nuclear Instruments and Methods in Physics Research B 3(1-3). p.373-376
Abstract
Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.

From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.

By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron... (More)
Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.

From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.

By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron bremsstrahlung. By low temperature ashing still better detection limits can be reached, but at the expense of the loss of volatile elements. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
serum proteins, gel filtration, PIXE, elemental composition
in
Nuclear Instruments and Methods in Physics Research B
volume
3
issue
1-3
pages
373 - 376
publisher
North-Holland
external identifiers
  • Scopus:25744477027
DOI
10.1016/0168-583X(84)90398-7
language
English
LU publication?
yes
id
b9508519-8e87-4bac-8dcc-638247c5a07d (old id 2204943)
date added to LUP
2011-11-18 18:02:47
date last changed
2016-10-13 04:49:26
@misc{b9508519-8e87-4bac-8dcc-638247c5a07d,
  abstract     = {Gel filtration of blood serum was carried out with Sephadex G-200 gel and tris buffer at pH 7.8. The amount of protein in each of about 100 collected fractions was monitored using UV absorption. The fractions were concentrated and deposited onto Kimfol backing foils.<br/><br>
From the PIXE results distributions of S, Fe, Cu and Zn were obtained in relation to the protein peaks, while elements like K, Ca and Br appeared at the position corresponding to small molecular sizes. Quantification, taking into account the intermediate thicknesses of the samples, gave reasonable values.<br/><br>
By adjusting the pH of the tris buffer with acetate instead of HCl, an increase in sensitivity was achieved, as the Cl is an important source of electron bremsstrahlung. By low temperature ashing still better detection limits can be reached, but at the expense of the loss of volatile elements.},
  author       = {Pallon, Jan and Pakarinen, Pirjo and Akselsson, Roland},
  keyword      = {serum proteins,gel filtration,PIXE,elemental composition},
  language     = {eng},
  number       = {1-3},
  pages        = {373--376},
  publisher    = {ARRAY(0xb56c278)},
  series       = {Nuclear Instruments and Methods in Physics Research B},
  title        = {PIXE Analysis of Blood Serum Proteins, Separated by Gel Filtration},
  url          = {http://dx.doi.org/10.1016/0168-583X(84)90398-7},
  volume       = {3},
  year         = {1984},
}