Advanced

Sampling, sample treatment, assay and real time monitoring of biotechnological processes using an integrated flow-injection system

Mazlomi, Mohammad LU (2011)
Abstract (Swedish)
Popular Abstract in English

Microorganisms can be used for production of pharmaceutical substances. With the

introduction of gene technology, it is possible to transfer information from one

organism to the other, thereby making the receiving organism capable of producing

substances that it did not so before. This development is very important for e.g.

production of therapeutic proteins

However, when using living organisms for production there is a need for controlling

a range of different parameters. In order to understand the process it is necessary

to have as much information as possible and then an algorithm to analyze the data.

In this thesis work... (More)
Popular Abstract in English

Microorganisms can be used for production of pharmaceutical substances. With the

introduction of gene technology, it is possible to transfer information from one

organism to the other, thereby making the receiving organism capable of producing

substances that it did not so before. This development is very important for e.g.

production of therapeutic proteins

However, when using living organisms for production there is a need for controlling

a range of different parameters. In order to understand the process it is necessary

to have as much information as possible and then an algorithm to analyze the data.

In this thesis work is presented that addresses some of the bottlenecks in

development of process monitoring. The coaxial catheter was evaluated with regard

to sampling from complex, sensitive medium without leakage of the inhibitory

solution that is used for instantaneous stopping of ongoing enzymatic/microbial

processes in the sample.

An automated system for advanced flow injection analysis of complex

biotechnological processes has been constructed and it was used to evaluate

efficiency of different inducer combinations when expressing a clone of enzyme.

One key factor in flow injection analysis is to prevent flow problems. The

introduction of supermacroporous hydrogels made it possible to introduce whole

broth from a fermenter into the system and also to include a superporous

catalytically active gel composed of microbial cells directly cross-linked to each

other.

Even if there is a need, still very few successful examples are presented where

advanced monitoring is used to control the processes. The missing part here is the

lack of a reliable, representative and robust sampling and sample handling system,

which is compatible with analytical devices.

The objective of this thesis work was to develop an aseptic and representative

sampling unit and treatment of the bioreactor product in the flow system, and

finally, analysing the sample by enzymatic and immunoassays in the integrated flow

system. (Less)
Abstract
Microorganisms can be used for production of pharmaceutical substances. With the

introduction of gene technology, it is possible to transfer information from one

organism to the other, thereby making the receiving organism capable of producing

substances that it did not so before. This development is very important for e.g.

production of therapeutic proteins

However, when using living organisms for production there is a need for controlling

a range of different parameters. In order to understand the process it is necessary

to have as much information as possible and then an algorithm to analyze the data.

In this thesis work is presented that addresses some of the... (More)
Microorganisms can be used for production of pharmaceutical substances. With the

introduction of gene technology, it is possible to transfer information from one

organism to the other, thereby making the receiving organism capable of producing

substances that it did not so before. This development is very important for e.g.

production of therapeutic proteins

However, when using living organisms for production there is a need for controlling

a range of different parameters. In order to understand the process it is necessary

to have as much information as possible and then an algorithm to analyze the data.

In this thesis work is presented that addresses some of the bottlenecks in

development of process monitoring. The coaxial catheter was evaluated with regard

to sampling from complex, sensitive medium without leakage of the inhibitory

solution that is used for instantaneous stopping of ongoing enzymatic/microbial

processes in the sample.

An automated system for advanced flow injection analysis of complex

biotechnological processes has been constructed and it was used to evaluate

efficiency of different inducer combinations when expressing a clone of enzyme.

One key factor in flow injection analysis is to prevent flow problems. The

introduction of supermacroporous hydrogels made it possible to introduce whole

broth from a fermenter into the system and also to include a superporous

catalytically active gel composed of microbial cells directly cross-linked to each

other.

Even if there is a need, still very few successful examples are presented where

advanced monitoring is used to control the processes. The missing part here is the

lack of a reliable, representative and robust sampling and sample handling system,

which is compatible with analytical devices.

The objective of this thesis work was to develop an aseptic and representative

sampling unit and treatment of the bioreactor product in the flow system, and

finally, analysing the sample by enzymatic and immunoassays in the integrated flow

system. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Håkansson, Håkan, GS Development, Malmö
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Bioprocess, Sampling, Sample treatment, On-line monitoring, Real time monitoring, Flow injection, Immunoassay, Fermentation
pages
132 pages
defense location
Lecture Hall B, Kemicentrum, Getingevägen 60, Lund University, Faculty of Engineering
defense date
2011-12-15 10:30
ISBN
978-91-89627-79-6
language
English
LU publication?
yes
id
516296ad-c0dd-46b2-9ff5-5c593a71e343 (old id 2205316)
date added to LUP
2011-11-21 10:58:53
date last changed
2016-09-19 08:45:19
@misc{516296ad-c0dd-46b2-9ff5-5c593a71e343,
  abstract     = {Microorganisms can be used for production of pharmaceutical substances. With the<br/><br>
introduction of gene technology, it is possible to transfer information from one<br/><br>
organism to the other, thereby making the receiving organism capable of producing<br/><br>
substances that it did not so before. This development is very important for e.g.<br/><br>
production of therapeutic proteins<br/><br>
However, when using living organisms for production there is a need for controlling<br/><br>
a range of different parameters. In order to understand the process it is necessary<br/><br>
to have as much information as possible and then an algorithm to analyze the data.<br/><br>
In this thesis work is presented that addresses some of the bottlenecks in<br/><br>
development of process monitoring. The coaxial catheter was evaluated with regard<br/><br>
to sampling from complex, sensitive medium without leakage of the inhibitory<br/><br>
solution that is used for instantaneous stopping of ongoing enzymatic/microbial<br/><br>
processes in the sample.<br/><br>
An automated system for advanced flow injection analysis of complex<br/><br>
biotechnological processes has been constructed and it was used to evaluate<br/><br>
efficiency of different inducer combinations when expressing a clone of enzyme.<br/><br>
One key factor in flow injection analysis is to prevent flow problems. The<br/><br>
introduction of supermacroporous hydrogels made it possible to introduce whole<br/><br>
broth from a fermenter into the system and also to include a superporous<br/><br>
catalytically active gel composed of microbial cells directly cross-linked to each<br/><br>
other.<br/><br>
Even if there is a need, still very few successful examples are presented where<br/><br>
advanced monitoring is used to control the processes. The missing part here is the<br/><br>
lack of a reliable, representative and robust sampling and sample handling system,<br/><br>
which is compatible with analytical devices.<br/><br>
The objective of this thesis work was to develop an aseptic and representative<br/><br>
sampling unit and treatment of the bioreactor product in the flow system, and<br/><br>
finally, analysing the sample by enzymatic and immunoassays in the integrated flow<br/><br>
system.},
  author       = {Mazlomi, Mohammad},
  isbn         = {978-91-89627-79-6},
  keyword      = {Bioprocess,Sampling,Sample treatment,On-line monitoring,Real time monitoring,Flow injection,Immunoassay,Fermentation},
  language     = {eng},
  pages        = {132},
  title        = {Sampling, sample treatment, assay and real time monitoring of biotechnological processes using an integrated flow-injection system},
  year         = {2011},
}