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Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Isaksson, Hanna LU ; Turunen, Mikael; Rieppo, Lassi; Saarakkala, Simo; Tamminen, Inari; Rieppo, Jarno; Kröger, Heikki and Jurvelin, Jukka (2010) In Journal of Bone and Mineral Research 25(6). p.1360-1366
Abstract
Renal osteodystrophy alters metabolic activity and remodeling rate of bone and also may lead to different bone composition. The objective of this study was to characterize the composition of bone in high‐turnover renal osteodystrophy patients by means of Fourier transform infrared spectroscopic imaging (FTIRI). Iliac crest biopsies from healthy bone (n = 11) and patients with renal osteodystrophy (ROD, n = 11) were used in this study. The ROD samples were from patients with hyperparathyroid disease. By using FTIRI, phosphate‐to‐amide I ratio (mineral‐to‐matrix ratio), carbonate‐to‐phosphate ratio, and carbonate‐to‐amide I ratio (turnover rate/remodeling activity), as well as the collagen cross‐link ratio (collagen maturity), were... (More)
Renal osteodystrophy alters metabolic activity and remodeling rate of bone and also may lead to different bone composition. The objective of this study was to characterize the composition of bone in high‐turnover renal osteodystrophy patients by means of Fourier transform infrared spectroscopic imaging (FTIRI). Iliac crest biopsies from healthy bone (n = 11) and patients with renal osteodystrophy (ROD, n = 11) were used in this study. The ROD samples were from patients with hyperparathyroid disease. By using FTIRI, phosphate‐to‐amide I ratio (mineral‐to‐matrix ratio), carbonate‐to‐phosphate ratio, and carbonate‐to‐amide I ratio (turnover rate/remodeling activity), as well as the collagen cross‐link ratio (collagen maturity), were quantified. Histomorphometric analyses were conducted for comparison. The ROD samples showed significantly lower carbonate‐to‐phosphate (p < .01) and carbonate‐to‐amide I (p < .001) ratios. The spatial variation across the trabeculae highlighted a significantly lower degree of mineralization (p < .05) at the edges of the trabeculae in the ROD samples than in normal bone. Statistically significant linear correlations were found between histomorphometric parameters related to bone‐remodeling activity and number of bone cells and FTIRI‐calculated parameters based on carbonate‐to‐phosphate and carbonate‐to‐amide I ratios. Hence the results suggested that FTIRI parameters related to carbonate may be indicative of turnover and remodeling rate of bone. © 2010 American Society for Bone and Mineral Research (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bone and Mineral Research
volume
25
issue
6
pages
1360 - 1366
publisher
AMBMR
external identifiers
  • Scopus:77953486660
ISSN
1523-4681
DOI
10.1002/jbmr.10
language
English
LU publication?
no
id
1b6d7491-27b0-40c3-b70d-27603ebcf7e3 (old id 2277109)
date added to LUP
2012-01-27 10:37:27
date last changed
2016-10-30 04:40:16
@misc{1b6d7491-27b0-40c3-b70d-27603ebcf7e3,
  abstract     = {Renal osteodystrophy alters metabolic activity and remodeling rate of bone and also may lead to different bone composition. The objective of this study was to characterize the composition of bone in high‐turnover renal osteodystrophy patients by means of Fourier transform infrared spectroscopic imaging (FTIRI). Iliac crest biopsies from healthy bone (n = 11) and patients with renal osteodystrophy (ROD, n = 11) were used in this study. The ROD samples were from patients with hyperparathyroid disease. By using FTIRI, phosphate‐to‐amide I ratio (mineral‐to‐matrix ratio), carbonate‐to‐phosphate ratio, and carbonate‐to‐amide I ratio (turnover rate/remodeling activity), as well as the collagen cross‐link ratio (collagen maturity), were quantified. Histomorphometric analyses were conducted for comparison. The ROD samples showed significantly lower carbonate‐to‐phosphate (p &lt; .01) and carbonate‐to‐amide I (p &lt; .001) ratios. The spatial variation across the trabeculae highlighted a significantly lower degree of mineralization (p &lt; .05) at the edges of the trabeculae in the ROD samples than in normal bone. Statistically significant linear correlations were found between histomorphometric parameters related to bone‐remodeling activity and number of bone cells and FTIRI‐calculated parameters based on carbonate‐to‐phosphate and carbonate‐to‐amide I ratios. Hence the results suggested that FTIRI parameters related to carbonate may be indicative of turnover and remodeling rate of bone. © 2010 American Society for Bone and Mineral Research},
  author       = {Isaksson, Hanna and Turunen, Mikael and Rieppo, Lassi and Saarakkala, Simo and Tamminen, Inari and Rieppo, Jarno and Kröger, Heikki and Jurvelin, Jukka},
  issn         = {1523-4681},
  language     = {eng},
  number       = {6},
  pages        = {1360--1366},
  publisher    = {ARRAY(0xb543878)},
  series       = {Journal of Bone and Mineral Research},
  title        = {Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy},
  url          = {http://dx.doi.org/10.1002/jbmr.10},
  volume       = {25},
  year         = {2010},
}