Precision of nanoindentation protocols for measurements of viscoelasticity in cortical and trabecular bone
Isaksson, Hanna LU
; Nagao, Shijo
; Malkiewicz, Marta
; Julkunen, Petro
; Nowak, Roman
and Jurvelin, Jukka
(2010)
In Journal of Biomechanics
43(12).
p.2410-2417
- Abstract
- Abstract in Undetermined
Nanoindentation has recently gained attention as a characterization technique for mechanical properties of biological tissues, such as bone, on the sub-micron level. However, optimal methods to characterize viscoelastic properties of bones are yet to be established. This study aimed to compare the time-dependent viscoelastic properties of bone tissue obtained with different nanoindentation methods. Bovine cortical and trabecular bone samples (n=8) from the distal femur and proximal tibia were dehydrated, embedded and polished. The material properties determined using nanoindentation were hardness and reduced modulus, as well as time-dependent parameters based on creep, loading-rate, dissipated energy and... (More) - Abstract in Undetermined
Nanoindentation has recently gained attention as a characterization technique for mechanical properties of biological tissues, such as bone, on the sub-micron level. However, optimal methods to characterize viscoelastic properties of bones are yet to be established. This study aimed to compare the time-dependent viscoelastic properties of bone tissue obtained with different nanoindentation methods. Bovine cortical and trabecular bone samples (n=8) from the distal femur and proximal tibia were dehydrated, embedded and polished. The material properties determined using nanoindentation were hardness and reduced modulus, as well as time-dependent parameters based on creep, loading-rate, dissipated energy and semi-dynamic testing under load control. Each loading protocol was repeated 160 times and the reproducibility was assessed based on the coefficient of variation (CV). Additionally, three well-characterized polymers were tested and CV values were calculated for reference.
The employed methods were able to characterize time-dependent viscoelastic properties of bone. However, their reproducibility varied highly (CV 9–40%). The creep constant increased with increasing dwell time. The reproducibility was best with a 30s creep period (CV 18%). The dissipated energy was stable after three repeated load cycles, and the reproducibility improved with each cycle (CV 23%). The viscoelastic properties determined with semi-dynamic test increased with increase in frequency. These measurements were most reproducible at high frequencies (CV 9–10%). Our results indicate that several methods are feasible for the determination of viscoelastic properties of bone material. The high frequency semi-dynamic test showed the highest precision within the tested nanoindentation protocols. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2277178
- author
- Isaksson, Hanna
LU
; Nagao, Shijo
; Malkiewicz, Marta
; Julkunen, Petro
; Nowak, Roman
and Jurvelin, Jukka
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biomechanics
- volume
- 43
- issue
- 12
- pages
- 2410 - 2417
- publisher
- Elsevier
- external identifiers
-
- scopus:77955555513
- ISSN
- 1873-2380
- DOI
- 10.1016/j.jbiomech.2010.04.017
- language
- English
- LU publication?
- no
- id
- ca1dd34f-9da4-4541-8bd6-8128146bbdba (old id 2277178)
- date added to LUP
- 2016-04-04 13:55:49
- date last changed
- 2023-09-06 19:05:45
@article{ca1dd34f-9da4-4541-8bd6-8128146bbdba,
abstract = {{Abstract in Undetermined<br/>Nanoindentation has recently gained attention as a characterization technique for mechanical properties of biological tissues, such as bone, on the sub-micron level. However, optimal methods to characterize viscoelastic properties of bones are yet to be established. This study aimed to compare the time-dependent viscoelastic properties of bone tissue obtained with different nanoindentation methods. Bovine cortical and trabecular bone samples (n=8) from the distal femur and proximal tibia were dehydrated, embedded and polished. The material properties determined using nanoindentation were hardness and reduced modulus, as well as time-dependent parameters based on creep, loading-rate, dissipated energy and semi-dynamic testing under load control. Each loading protocol was repeated 160 times and the reproducibility was assessed based on the coefficient of variation (CV). Additionally, three well-characterized polymers were tested and CV values were calculated for reference.<br/><br/>The employed methods were able to characterize time-dependent viscoelastic properties of bone. However, their reproducibility varied highly (CV 9–40%). The creep constant increased with increasing dwell time. The reproducibility was best with a 30s creep period (CV 18%). The dissipated energy was stable after three repeated load cycles, and the reproducibility improved with each cycle (CV 23%). The viscoelastic properties determined with semi-dynamic test increased with increase in frequency. These measurements were most reproducible at high frequencies (CV 9–10%). Our results indicate that several methods are feasible for the determination of viscoelastic properties of bone material. The high frequency semi-dynamic test showed the highest precision within the tested nanoindentation protocols.}},
author = {{Isaksson, Hanna and Nagao, Shijo and Malkiewicz, Marta and Julkunen, Petro and Nowak, Roman and Jurvelin, Jukka}},
issn = {{1873-2380}},
language = {{eng}},
number = {{12}},
pages = {{2410--2417}},
publisher = {{Elsevier}},
series = {{Journal of Biomechanics}},
title = {{Precision of nanoindentation protocols for measurements of viscoelasticity in cortical and trabecular bone}},
url = {{http://dx.doi.org/10.1016/j.jbiomech.2010.04.017}},
doi = {{10.1016/j.jbiomech.2010.04.017}},
volume = {{43}},
year = {{2010}},
}