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Antibody Development - Examples from viral and proteomic studies

Carlsson, Fredrika LU (2012)
Abstract
This thesis, which is based on four original papers, covers different aspects of antibody development. The overall goal of my work has been to obtain an increased understanding of antibody repertoires as they develop in vivo and in vitro.

In Paper I-III antibody repertoires was evaluated and used to dissect virus-induced antibody responses and characterize such antibodies. Furthermore, tools for more efficient studies of such characterized antibodies were developed. The study of antibody repertoires in this thesis reveals, not only insights of the composition of antibodies induced by a common virus, but also features for such binders developed by combinatorial and non-combinatorial approaches. Such developed antibodies may... (More)
This thesis, which is based on four original papers, covers different aspects of antibody development. The overall goal of my work has been to obtain an increased understanding of antibody repertoires as they develop in vivo and in vitro.

In Paper I-III antibody repertoires was evaluated and used to dissect virus-induced antibody responses and characterize such antibodies. Furthermore, tools for more efficient studies of such characterized antibodies were developed. The study of antibody repertoires in this thesis reveals, not only insights of the composition of antibodies induced by a common virus, but also features for such binders developed by combinatorial and non-combinatorial approaches. Such developed antibodies may contribute with data important for future design of vaccine candidates.

In Paper IV, limitations in biomarker verification processes are addressed. A proof-of-concept study was developed, describing an approach with high-throughput potential relying on enrichment of proteins by antibody fragments in complex serum samples followed by an MRM mass spectrometry readout. Taken together, the in vitro developed binders in combination with MRM readout provides a powerful method to quantify protein biomarkers in a high-throughput manner approaching the criteria for clinical usage. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Persson, Mats, Karolinska institutet, Stockholm, Sweden
organization
publishing date
type
Thesis
publication status
published
subject
keywords
proteome specific antibodies, cytomegalovirus, phage display, antibody characterization, antibody development, antibody repertoires
defense location
Room Belfragesalen BMC D15, Sölvegatan 19, Lund, Sweden
defense date
2012-03-23 13:15
ISBN
978-91-7473-276-4
language
English
LU publication?
yes
id
08621ef5-7c3a-41a3-b83b-6d47bcb5c994 (old id 2364788)
date added to LUP
2012-02-27 12:20:03
date last changed
2016-09-19 08:45:18
@misc{08621ef5-7c3a-41a3-b83b-6d47bcb5c994,
  abstract     = {This thesis, which is based on four original papers, covers different aspects of antibody development. The overall goal of my work has been to obtain an increased understanding of antibody repertoires as they develop in vivo and in vitro.<br/><br>
In Paper I-III antibody repertoires was evaluated and used to dissect virus-induced antibody responses and characterize such antibodies. Furthermore, tools for more efficient studies of such characterized antibodies were developed. The study of antibody repertoires in this thesis reveals, not only insights of the composition of antibodies induced by a common virus, but also features for such binders developed by combinatorial and non-combinatorial approaches. Such developed antibodies may contribute with data important for future design of vaccine candidates.<br/><br>
In Paper IV, limitations in biomarker verification processes are addressed. A proof-of-concept study was developed, describing an approach with high-throughput potential relying on enrichment of proteins by antibody fragments in complex serum samples followed by an MRM mass spectrometry readout. Taken together, the in vitro developed binders in combination with MRM readout provides a powerful method to quantify protein biomarkers in a high-throughput manner approaching the criteria for clinical usage.},
  author       = {Carlsson, Fredrika},
  isbn         = {978-91-7473-276-4},
  keyword      = {proteome specific antibodies,cytomegalovirus,phage display,antibody characterization,antibody development,antibody repertoires},
  language     = {eng},
  title        = {Antibody Development - Examples from viral and proteomic studies},
  year         = {2012},
}