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Amphetamine-induced rotation and l-DOPA-induced dyskinesia in the rat 6-OHDA model: A correlation study.

Tronci, Elisabetta; Shin, Eunju LU ; Björklund, Anders LU and Carta, Manolo LU (2012) In Neuroscience Research 73(2). p.168-172
Abstract
The present study investigated whether the rotation rate induced by amphetamine in 6-OHDA-lesioned rats was predictive of development of l-DOPA-induced dyskinesia (LID) and success of the lesion procedure in our experimental settings. We collected data from 312 6-OHDA-lesioned rats (from different sets of experiments). Rats were subjected to the amphetamine-induced rotation test (2.5mg/kg) and chronically treated with l-DOPA (6mg/kg) to establish dyskinesia. A poor correlation was present between amphetamine-induced rotation and LID. Moreover, no correlation was found between amphetamine-induced rotation and tyrosine hydroxylase (TH) positive cell number in the lesioned substantia nigra pars compacta, while there was a weak correlation... (More)
The present study investigated whether the rotation rate induced by amphetamine in 6-OHDA-lesioned rats was predictive of development of l-DOPA-induced dyskinesia (LID) and success of the lesion procedure in our experimental settings. We collected data from 312 6-OHDA-lesioned rats (from different sets of experiments). Rats were subjected to the amphetamine-induced rotation test (2.5mg/kg) and chronically treated with l-DOPA (6mg/kg) to establish dyskinesia. A poor correlation was present between amphetamine-induced rotation and LID. Moreover, no correlation was found between amphetamine-induced rotation and tyrosine hydroxylase (TH) positive cell number in the lesioned substantia nigra pars compacta, while there was a weak correlation between the percentage of TH positive cell number and LID. These results indicate that the amphetamine-induced rotation test is a poor predictor of the 6-OHDA-lesion success, as well as of the development of LID at the dose of amphetamine used here. Our data also suggest that all rats with amphetamine-induced rotation ≥3turns/min should be included in dyskinesia studies, as they showed the same propensity to develop dyskinesia. Moreover, SERT expression levels suggest that reduced striatal and pallidal serotonin innervation might have contributed to the lower dyskinesia levels observed in a subset of amphetamine-responsive rats. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Neuroscience Research
volume
73
issue
2
pages
168 - 172
publisher
Elsevier
external identifiers
  • WOS:000304520200010
  • PMID:22450171
  • Scopus:84860333674
ISSN
0168-0102
DOI
10.1016/j.neures.2012.03.004
language
English
LU publication?
yes
id
2163ac5b-21ca-4ff8-aa4b-9d6f06e262c2 (old id 2431440)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22450171?dopt=Abstract
date added to LUP
2012-04-03 13:48:16
date last changed
2016-11-24 15:37:04
@misc{2163ac5b-21ca-4ff8-aa4b-9d6f06e262c2,
  abstract     = {The present study investigated whether the rotation rate induced by amphetamine in 6-OHDA-lesioned rats was predictive of development of l-DOPA-induced dyskinesia (LID) and success of the lesion procedure in our experimental settings. We collected data from 312 6-OHDA-lesioned rats (from different sets of experiments). Rats were subjected to the amphetamine-induced rotation test (2.5mg/kg) and chronically treated with l-DOPA (6mg/kg) to establish dyskinesia. A poor correlation was present between amphetamine-induced rotation and LID. Moreover, no correlation was found between amphetamine-induced rotation and tyrosine hydroxylase (TH) positive cell number in the lesioned substantia nigra pars compacta, while there was a weak correlation between the percentage of TH positive cell number and LID. These results indicate that the amphetamine-induced rotation test is a poor predictor of the 6-OHDA-lesion success, as well as of the development of LID at the dose of amphetamine used here. Our data also suggest that all rats with amphetamine-induced rotation ≥3turns/min should be included in dyskinesia studies, as they showed the same propensity to develop dyskinesia. Moreover, SERT expression levels suggest that reduced striatal and pallidal serotonin innervation might have contributed to the lower dyskinesia levels observed in a subset of amphetamine-responsive rats.},
  author       = {Tronci, Elisabetta and Shin, Eunju and Björklund, Anders and Carta, Manolo},
  issn         = {0168-0102},
  language     = {eng},
  number       = {2},
  pages        = {168--172},
  publisher    = {ARRAY(0xabe42d0)},
  series       = {Neuroscience Research},
  title        = {Amphetamine-induced rotation and l-DOPA-induced dyskinesia in the rat 6-OHDA model: A correlation study.},
  url          = {http://dx.doi.org/10.1016/j.neures.2012.03.004},
  volume       = {73},
  year         = {2012},
}