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Immunoradiometric assay of inhibitors of antihaemophilic factor A

Ljung, Rolf LU and Holmberg, Lars LU (1982) In Acta Paediatrica Scandinavica 71(6). p.1019-1023
Abstract
An immunoradiometric assay (IRMA) for determination of antibodies against f. VIII:C in haemophilia A was developed. The assay was based on competitive binding of radiolabelled anti-VIII:C and antibodies in the test material to immobilized VIII:C. Fifteen haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. In 3 cases the inhibitors were detected only with the IRMA and in the other 12 there was good agreement between the inhibitor levels found with the two methods. It was also possible to demonstrate the antibodies in three non-haemophilic patients with acquired inhibitors. The IRMA, which can detect the antibodies down to a concentration of 0.02 inhibitor units per ml, is... (More)
An immunoradiometric assay (IRMA) for determination of antibodies against f. VIII:C in haemophilia A was developed. The assay was based on competitive binding of radiolabelled anti-VIII:C and antibodies in the test material to immobilized VIII:C. Fifteen haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. In 3 cases the inhibitors were detected only with the IRMA and in the other 12 there was good agreement between the inhibitor levels found with the two methods. It was also possible to demonstrate the antibodies in three non-haemophilic patients with acquired inhibitors. The IRMA, which can detect the antibodies down to a concentration of 0.02 inhibitor units per ml, is more sensitive than conventional neutralization tests and is thus of practical importance in the investigation of patients with low inhibitor titres. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
blood clotting factor 8, radioisotope, blood and hemopoietic system, case report, congenital disorder, diagnosis, hemophilia A, radioimmunoassay
in
Acta Paediatrica Scandinavica
volume
71
issue
6
pages
5 pages
external identifiers
  • Scopus:0019972838
ISSN
0001-656X
language
English
LU publication?
yes
id
2481bd57-ef97-4616-89c4-313d55b03d55
date added to LUP
2016-10-26 13:25:05
date last changed
2016-10-30 04:50:56
@misc{2481bd57-ef97-4616-89c4-313d55b03d55,
  abstract     = {An immunoradiometric assay (IRMA) for determination of antibodies against f. VIII:C in haemophilia A was developed. The assay was based on competitive binding of radiolabelled anti-VIII:C and antibodies in the test material to immobilized VIII:C. Fifteen haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. In 3 cases the inhibitors were detected only with the IRMA and in the other 12 there was good agreement between the inhibitor levels found with the two methods. It was also possible to demonstrate the antibodies in three non-haemophilic patients with acquired inhibitors. The IRMA, which can detect the antibodies down to a concentration of 0.02 inhibitor units per ml, is more sensitive than conventional neutralization tests and is thus of practical importance in the investigation of patients with low inhibitor titres.},
  author       = {Ljung, Rolf and Holmberg, Lars},
  issn         = {0001-656X},
  keyword      = {blood clotting factor 8,radioisotope,blood and hemopoietic system,case report,congenital disorder,diagnosis,hemophilia A,radioimmunoassay},
  language     = {eng},
  month        = {10},
  number       = {6},
  pages        = {1019--1023},
  series       = {Acta Paediatrica Scandinavica},
  title        = {Immunoradiometric assay of inhibitors of antihaemophilic factor A},
  volume       = {71},
  year         = {1982},
}