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Autofluorescence of pigmented skin lesions using a pulsed UV laser

Cheng, Haynes P. H.; Svenmarker, Pontus LU ; Xie, Haiyan LU ; Tidemand-Lichtenberg, Peter; Jensen, Ole B.; Bendsöe, Niels LU ; Svanberg, Katarina LU ; Petersen, Paul Michael; Pedersen, Christian and Andersson-Engels, Stefan LU , et al. (2010) In Proceedings of SPIE 7715. p.1-77151
Abstract
We report preliminary clinical results of autofluorescence imaging of malignant and benign skin lesions, using pulsed

355 nm laser excitation with synchronized detection. The novel synchronized detection system allows high signal-tonoise

ratio to be achieved in the resulting autofluorescence signal, which may in turn produce high contrast images that

improve diagnosis, even in the presence of ambient room light. The synchronized set-up utilizes a compact, diode

pumped, pulsed UV laser at 355 nm which is coupled to a CCD camera and a liquid crystal tunable filter. The excitation

and image capture is sampled at 5 kHz and the resulting autofluorescence is captured with the liquid crystal filter... (More)
We report preliminary clinical results of autofluorescence imaging of malignant and benign skin lesions, using pulsed

355 nm laser excitation with synchronized detection. The novel synchronized detection system allows high signal-tonoise

ratio to be achieved in the resulting autofluorescence signal, which may in turn produce high contrast images that

improve diagnosis, even in the presence of ambient room light. The synchronized set-up utilizes a compact, diode

pumped, pulsed UV laser at 355 nm which is coupled to a CCD camera and a liquid crystal tunable filter. The excitation

and image capture is sampled at 5 kHz and the resulting autofluorescence is captured with the liquid crystal filter cycling

through seven wavelengths between 420 nm and 580 nm. The clinical study targets pigmented skin lesions and evaluates

the prospects of using autofluorescence as a possible means in differentiating malignant and benign skin tumors. Up to

now, sixteen patients have participated in the clinical study. The autofluorescence images, averaged over the exposure

time of one second, will be presented along with histopathological results. Initial survey of the images show good

contrast and diagnostic results show promising agreement based on the histopathological results. (Less)
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
autofluorescence imaging, photodynamic therapy, skin lesion, diagnostic
in
Proceedings of SPIE
volume
7715
pages
1 - 77151
publisher
SPIE
external identifiers
  • Scopus:78751606332
DOI
10.1117/12.855837
language
English
LU publication?
yes
id
7d6549cd-4aad-4071-b8f0-5a27fbd5d226 (old id 2596013)
date added to LUP
2012-05-29 16:17:49
date last changed
2016-10-13 04:36:44
@misc{7d6549cd-4aad-4071-b8f0-5a27fbd5d226,
  abstract     = {We report preliminary clinical results of autofluorescence imaging of malignant and benign skin lesions, using pulsed<br/><br>
355 nm laser excitation with synchronized detection. The novel synchronized detection system allows high signal-tonoise<br/><br>
ratio to be achieved in the resulting autofluorescence signal, which may in turn produce high contrast images that<br/><br>
improve diagnosis, even in the presence of ambient room light. The synchronized set-up utilizes a compact, diode<br/><br>
pumped, pulsed UV laser at 355 nm which is coupled to a CCD camera and a liquid crystal tunable filter. The excitation<br/><br>
and image capture is sampled at 5 kHz and the resulting autofluorescence is captured with the liquid crystal filter cycling<br/><br>
through seven wavelengths between 420 nm and 580 nm. The clinical study targets pigmented skin lesions and evaluates<br/><br>
the prospects of using autofluorescence as a possible means in differentiating malignant and benign skin tumors. Up to<br/><br>
now, sixteen patients have participated in the clinical study. The autofluorescence images, averaged over the exposure<br/><br>
time of one second, will be presented along with histopathological results. Initial survey of the images show good<br/><br>
contrast and diagnostic results show promising agreement based on the histopathological results.},
  author       = {Cheng, Haynes P. H. and Svenmarker, Pontus and Xie, Haiyan and Tidemand-Lichtenberg, Peter and Jensen, Ole B. and Bendsöe, Niels and Svanberg, Katarina and Petersen, Paul Michael and Pedersen, Christian and Andersson-Engels, Stefan and Andersen, Peter E.},
  keyword      = {autofluorescence imaging,photodynamic therapy,skin lesion,diagnostic},
  language     = {eng},
  pages        = {1--77151},
  publisher    = {ARRAY(0xbf72c00)},
  series       = {Proceedings of SPIE},
  title        = {Autofluorescence of pigmented skin lesions using a pulsed UV laser},
  url          = {http://dx.doi.org/10.1117/12.855837},
  volume       = {7715},
  year         = {2010},
}