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Studies on pancreatic secretory trypsin inhibitor in normal and diseased gastrointestinal tract

Bohe, Hans (1996)
Abstract
The presence of immunoreactive pancreatic secretory trypsin inhibitor (irPSTI) was studied using an immunohistochemical method in normal and diseased gall bladder and normal and neoplastic colonic mucosa. Normal and inflammatory gall bladder mucosa did not stain for irPSTI. Neoplastic gall bldder mucosa showed positive staining for irPSTI, with a more pronounced staining in well rather than in poorly-diferentiated carcinoma. Normal colonic mucosa and adenomas, but rarely colonic carcinoma, stained positive for irPSTI. Furthermore, irPSTI, measured with an enzyme-linked immunosorbant assay (ELISA), revealed a jigher content in normal than in neoplastic colonic mucosa. These results indicatea prduction and consumption of PSTI in neoplastic... (More)
The presence of immunoreactive pancreatic secretory trypsin inhibitor (irPSTI) was studied using an immunohistochemical method in normal and diseased gall bladder and normal and neoplastic colonic mucosa. Normal and inflammatory gall bladder mucosa did not stain for irPSTI. Neoplastic gall bldder mucosa showed positive staining for irPSTI, with a more pronounced staining in well rather than in poorly-diferentiated carcinoma. Normal colonic mucosa and adenomas, but rarely colonic carcinoma, stained positive for irPSTI. Furthermore, irPSTI, measured with an enzyme-linked immunosorbant assay (ELISA), revealed a jigher content in normal than in neoplastic colonic mucosa. These results indicatea prduction and consumption of PSTI in neoplastic tissue. IrPSTI was identified in most Brunner´s glands and the distribution was compared with that of ir Lysozyme and mucus. Using an ELISA and reverse transcriptase polymerase chain reaction ( RT-PCR), production and secretion of irPSTI was ascertained in human small intestine. The physiological function of PSTI may be epithelial cell protection. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Professor Sjödahl, Rune
publishing date
type
Thesis
publication status
published
subject
keywords
Surgery, small intestinal secretion, reverse transcriptase, polymerase chain reaction, neoplastic tissue, immunohistology, Brunner´s glands, gall bladder, orthopaedics, traumatology, Kirurgi, ortopedi, traumatologi
pages
83 pages
defense location
The lecture hall, Department of Surgery, entrance 42
defense date
1996-06-04 09:15
external identifiers
  • Other:ISRN: LUMEDW/MEKP-1023-SE
language
English
LU publication?
no
id
ef987335-bc05-4fce-a958-3e9c1cdfd403 (old id 28493)
date added to LUP
2007-06-11 14:59:52
date last changed
2016-09-19 08:45:19
@misc{ef987335-bc05-4fce-a958-3e9c1cdfd403,
  abstract     = {The presence of immunoreactive pancreatic secretory trypsin inhibitor (irPSTI) was studied using an immunohistochemical method in normal and diseased gall bladder and normal and neoplastic colonic mucosa. Normal and inflammatory gall bladder mucosa did not stain for irPSTI. Neoplastic gall bldder mucosa showed positive staining for irPSTI, with a more pronounced staining in well rather than in poorly-diferentiated carcinoma. Normal colonic mucosa and adenomas, but rarely colonic carcinoma, stained positive for irPSTI. Furthermore, irPSTI, measured with an enzyme-linked immunosorbant assay (ELISA), revealed a jigher content in normal than in neoplastic colonic mucosa. These results indicatea prduction and consumption of PSTI in neoplastic tissue. IrPSTI was identified in most Brunner´s glands and the distribution was compared with that of ir Lysozyme and mucus. Using an ELISA and reverse transcriptase polymerase chain reaction ( RT-PCR), production and secretion of irPSTI was ascertained in human small intestine. The physiological function of PSTI may be epithelial cell protection.},
  author       = {Bohe, Hans},
  keyword      = {Surgery,small intestinal secretion,reverse transcriptase,polymerase chain reaction,neoplastic tissue,immunohistology,Brunner´s glands,gall bladder,orthopaedics,traumatology,Kirurgi,ortopedi,traumatologi},
  language     = {eng},
  pages        = {83},
  title        = {Studies on pancreatic secretory trypsin inhibitor in normal and diseased gastrointestinal tract},
  year         = {1996},
}