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Expression of Ig genes. Regulation of transcription and production of human antibodies

Furebring, Christina LU (1996)
Abstract
During B lymphocyte development, the transcriptional activity of the IgH locus is subject to spatial and temporal changes. The 3' enhancer (3'E) has been suggested to play an important role in regulation of immunoglobulin gene expression late in B cell development. We have investigated, using transgenic mice, the role of the 3'E in regulating Ig gene expression. Mice harbouring a rearranged IgH gene potentiated by the VH promoter in combination with the IgH intron enhancer (µE), the 3'E or the µE/3'E pair were generated. The 3'E activity is mainly observed in lymphoid tissues and is mainly restricted to the in vivo activated B cells. The 3'E can potentiate Ig gene expression directly in conjunction with the VH promoter. The expression... (More)
During B lymphocyte development, the transcriptional activity of the IgH locus is subject to spatial and temporal changes. The 3' enhancer (3'E) has been suggested to play an important role in regulation of immunoglobulin gene expression late in B cell development. We have investigated, using transgenic mice, the role of the 3'E in regulating Ig gene expression. Mice harbouring a rearranged IgH gene potentiated by the VH promoter in combination with the IgH intron enhancer (µE), the 3'E or the µE/3'E pair were generated. The 3'E activity is mainly observed in lymphoid tissues and is mainly restricted to the in vivo activated B cells. The 3'E can potentiate Ig gene expression directly in conjunction with the VH promoter. The expression level of the µE/3'E controlled transgene is fivefold higher as compared to the transgene controlled by the µE alone. The aim of my subsequent studies has been to generate and produce human monoclonal antibodies. We have focused our interest on immortalization of the variable region genes, from hybridoma or from a single antigen-specific B cell, using the powerful PCR technique. The variable region genes from single B cells can be immortalized directly or after a cellular amplification step, involving the EL-4 and CD40 cell culture systems. The variable region genes obtained can thereafter be expressed either as Ab fragments, in prokaryotic host cells, or as the entire Ab, in eukaryotic host cells. To allow efficient expression of intact Ab we have optimized a eukaryotic IgH gene expression vector using different combinations of regulatory elements. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof. Sandlie, Inger, department of Biology, Oslo University
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Immunology, Bioteknik, Biotechnology, human antibodies, immunoglobulin gene expression, 3' enhancer, serology, transplantation, Immunologi, serologi
pages
43 pages
publisher
Department of Immunotechnology, Lund University
defense location
KC hall D.
defense date
1996-06-14 10:00
external identifiers
  • Other:ISRN: LUTKDH/TKIT--96/1002
ISBN
91-628-2056-7
language
English
LU publication?
yes
id
306d8299-355f-4604-b7df-6f103fbe21f2 (old id 28581)
date added to LUP
2007-06-13 14:03:47
date last changed
2016-09-19 08:45:05
@misc{306d8299-355f-4604-b7df-6f103fbe21f2,
  abstract     = {During B lymphocyte development, the transcriptional activity of the IgH locus is subject to spatial and temporal changes. The 3' enhancer (3'E) has been suggested to play an important role in regulation of immunoglobulin gene expression late in B cell development. We have investigated, using transgenic mice, the role of the 3'E in regulating Ig gene expression. Mice harbouring a rearranged IgH gene potentiated by the VH promoter in combination with the IgH intron enhancer (µE), the 3'E or the µE/3'E pair were generated. The 3'E activity is mainly observed in lymphoid tissues and is mainly restricted to the in vivo activated B cells. The 3'E can potentiate Ig gene expression directly in conjunction with the VH promoter. The expression level of the µE/3'E controlled transgene is fivefold higher as compared to the transgene controlled by the µE alone. The aim of my subsequent studies has been to generate and produce human monoclonal antibodies. We have focused our interest on immortalization of the variable region genes, from hybridoma or from a single antigen-specific B cell, using the powerful PCR technique. The variable region genes from single B cells can be immortalized directly or after a cellular amplification step, involving the EL-4 and CD40 cell culture systems. The variable region genes obtained can thereafter be expressed either as Ab fragments, in prokaryotic host cells, or as the entire Ab, in eukaryotic host cells. To allow efficient expression of intact Ab we have optimized a eukaryotic IgH gene expression vector using different combinations of regulatory elements.},
  author       = {Furebring, Christina},
  isbn         = {91-628-2056-7},
  keyword      = {Immunology,Bioteknik,Biotechnology,human antibodies,immunoglobulin gene expression,3' enhancer,serology,transplantation,Immunologi,serologi},
  language     = {eng},
  pages        = {43},
  publisher    = {ARRAY(0x97829f0)},
  title        = {Expression of Ig genes. Regulation of transcription and production of human antibodies},
  year         = {1996},
}