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Pituitary adenylate cyclase activating peptide: a neuropeptide in the sensory nervous system.

Zhang, Yanzhen (1996)
Abstract
This thesis deals with the expression of pituitary adenylate cyclase activating peptide (PACAP) in sensory C-type cells and primary afferent fibres and its possible role in nociceptive transmission. PACAP-expression, -release, -synthesis and -function were investigated in a series of measurements using immunocytochemistry, radioimmunoassay, in situ hybridization histochemistry, Northern blot, electrophysiology, superficial spinal perfusion and nerve injury. PACAP expresses normally in C-type sensory neurons and primary afferent fibres in rats and coexists with SP/CGRP in nerve fibres of the superficial laminae of the dorsal horn and of the peripheral tissues, of organs with prominent sensory innervation. Capsaicin releases PACAP from the... (More)
This thesis deals with the expression of pituitary adenylate cyclase activating peptide (PACAP) in sensory C-type cells and primary afferent fibres and its possible role in nociceptive transmission. PACAP-expression, -release, -synthesis and -function were investigated in a series of measurements using immunocytochemistry, radioimmunoassay, in situ hybridization histochemistry, Northern blot, electrophysiology, superficial spinal perfusion and nerve injury. PACAP expresses normally in C-type sensory neurons and primary afferent fibres in rats and coexists with SP/CGRP in nerve fibres of the superficial laminae of the dorsal horn and of the peripheral tissues, of organs with prominent sensory innervation. Capsaicin releases PACAP from the central terminals of primary C-type afferents in the spinal cord, supporting the view that PACAP participates in transmission in the spinal dorsal horn as a messenger. Intrathecal administration of PACAP suppresses the C-fibre evoked flexion reflex and formalin-induced pain-related behaviour. Taken together, the evidence shows that PACAP may act as a modulator in nociception and has antinociceptive effects. Following sciatic nerve injury, the synthesis of PACAP in the dorsal root ganglia is upregulated and the concentrations of PACAP in the spinal cord and the proximal nerve stumps enhanced, indicating that PACAP plays a role in the postaxotomy repair and restoration process. It may act as a neurotropic factor or as a survival factor in adaptation to nerve injury. The findings are consistent with the hypothesis that PACAP may be important both in sensory transmission and in regeneration. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Professor Hedner, Thomas, Sahlgrenska Sjukhuset, Göteborg
publishing date
type
Thesis
publication status
published
subject
keywords
in situ hybridization, immunocytochemistry, peptide expression, inflammation, nerve injury, axotomy, capsaicin, pain, nociceptive, formalin test, C-fibres, sensory neurons, spinal cord, dorsal root ganglia, mRNA, neuropeptides, VIP, SP, PACAP, CGRP, Northern blot, rat, Neurology, neuropsychology, neurophysiology, Neurologi, neuropsykologi, neurofysiologi
pages
168 pages
defense location
Segerfalk Lecture Hall, Wallenberg Neuroscience Center, Sölvegatan 17, Lund. (13.15)
defense date
1999-09-19 10:15
external identifiers
  • Other:ISRN: LUMEDW/MEFN--03--SE
ISBN
91-628-2160-1
language
English
LU publication?
no
id
661e80d1-a545-4e58-a8e3-f75bb2431070 (old id 28628)
date added to LUP
2007-06-12 13:50:00
date last changed
2016-09-19 08:45:16
@misc{661e80d1-a545-4e58-a8e3-f75bb2431070,
  abstract     = {This thesis deals with the expression of pituitary adenylate cyclase activating peptide (PACAP) in sensory C-type cells and primary afferent fibres and its possible role in nociceptive transmission. PACAP-expression, -release, -synthesis and -function were investigated in a series of measurements using immunocytochemistry, radioimmunoassay, in situ hybridization histochemistry, Northern blot, electrophysiology, superficial spinal perfusion and nerve injury. PACAP expresses normally in C-type sensory neurons and primary afferent fibres in rats and coexists with SP/CGRP in nerve fibres of the superficial laminae of the dorsal horn and of the peripheral tissues, of organs with prominent sensory innervation. Capsaicin releases PACAP from the central terminals of primary C-type afferents in the spinal cord, supporting the view that PACAP participates in transmission in the spinal dorsal horn as a messenger. Intrathecal administration of PACAP suppresses the C-fibre evoked flexion reflex and formalin-induced pain-related behaviour. Taken together, the evidence shows that PACAP may act as a modulator in nociception and has antinociceptive effects. Following sciatic nerve injury, the synthesis of PACAP in the dorsal root ganglia is upregulated and the concentrations of PACAP in the spinal cord and the proximal nerve stumps enhanced, indicating that PACAP plays a role in the postaxotomy repair and restoration process. It may act as a neurotropic factor or as a survival factor in adaptation to nerve injury. The findings are consistent with the hypothesis that PACAP may be important both in sensory transmission and in regeneration.},
  author       = {Zhang, Yanzhen},
  isbn         = {91-628-2160-1},
  keyword      = {in situ hybridization,immunocytochemistry,peptide expression,inflammation,nerve injury,axotomy,capsaicin,pain,nociceptive,formalin test,C-fibres,sensory neurons,spinal cord,dorsal root ganglia,mRNA,neuropeptides,VIP,SP,PACAP,CGRP,Northern blot,rat,Neurology,neuropsychology,neurophysiology,Neurologi,neuropsykologi,neurofysiologi},
  language     = {eng},
  pages        = {168},
  title        = {Pituitary adenylate cyclase activating peptide: a neuropeptide in the sensory nervous system.},
  year         = {1996},
}