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Procedures for Sample Clean-up and Concentration in Capillary Zone Electrophoresis for Determination of Drugs in Biosamples

Pálmarsdóttir, Sveinbjörg (1996)
Abstract
Procedures for sample clean-up and concentration in Capillary Zone Electrophoresis (CZE) have been developed for determination of drugs in biosample s. An on-capillary concentration technique based on enhanced field strengths, the double stacking procedure is introduced. This procedure allows for concentrationg all cations in the sample several hundred times essentially without loss of separation performance. This technique is fully comptible with the clean and low ionic strength extracts obtainable from treating plasma samples with the novel supported liquid membrane (SLM) sample pretreatment technique. Both off- and on-line approaches are presented for the combination of the SLM technique with the double stacking CZE. In the simplest... (More)
Procedures for sample clean-up and concentration in Capillary Zone Electrophoresis (CZE) have been developed for determination of drugs in biosample s. An on-capillary concentration technique based on enhanced field strengths, the double stacking procedure is introduced. This procedure allows for concentrationg all cations in the sample several hundred times essentially without loss of separation performance. This technique is fully comptible with the clean and low ionic strength extracts obtainable from treating plasma samples with the novel supported liquid membrane (SLM) sample pretreatment technique. Both off- and on-line approaches are presented for the combination of the SLM technique with the double stacking CZE. In the simplest approach the SLM technique is used in an off-line combination with ZCE. Only a part of the SLM extract is used for the final analysis by CZE and the enrichment factor obtained is more than 1000 times. In another approach a comparable enrichment factor was obtained by connecting a minituarized SLM device on-line with CZE. The whole extracted sample volume could now be injected into the CZE capillary. In the third approach the SLM device is connected via a CLC-microcolumn system on-line to the CZE system. This system being the most complicated one gives enrichment factors around 40 000 times and very high selectivity towards matrix components.



Cyclodextrins were explored for the separation of enantiomers of drugs. The chiral separation systems were compatible with the CZE double stacking procedure and were used for the determination of drug enantiomers in biosamples. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Lloyd, David, Ph D, Canada
publishing date
type
Thesis
publication status
published
subject
keywords
Analytical chemistry, Analytisk kemi
pages
58 pages
defense location
The Chemical Center
defense date
1996-10-03 10:15
external identifiers
  • Other:ISRN: LUNDKL/ (NKAK-1036)/-(1996)
language
English
LU publication?
no
id
3a92a725-6e10-45c2-9845-00b1628879dd (old id 28659)
date added to LUP
2007-06-12 09:11:17
date last changed
2016-09-19 08:45:16
@misc{3a92a725-6e10-45c2-9845-00b1628879dd,
  abstract     = {Procedures for sample clean-up and concentration in Capillary Zone Electrophoresis (CZE) have been developed for determination of drugs in biosample s. An on-capillary concentration technique based on enhanced field strengths, the double stacking procedure is introduced. This procedure allows for concentrationg all cations in the sample several hundred times essentially without loss of separation performance. This technique is fully comptible with the clean and low ionic strength extracts obtainable from treating plasma samples with the novel supported liquid membrane (SLM) sample pretreatment technique. Both off- and on-line approaches are presented for the combination of the SLM technique with the double stacking CZE. In the simplest approach the SLM technique is used in an off-line combination with ZCE. Only a part of the SLM extract is used for the final analysis by CZE and the enrichment factor obtained is more than 1000 times. In another approach a comparable enrichment factor was obtained by connecting a minituarized SLM device on-line with CZE. The whole extracted sample volume could now be injected into the CZE capillary. In the third approach the SLM device is connected via a CLC-microcolumn system on-line to the CZE system. This system being the most complicated one gives enrichment factors around 40 000 times and very high selectivity towards matrix components.<br/><br>
<br/><br>
Cyclodextrins were explored for the separation of enantiomers of drugs. The chiral separation systems were compatible with the CZE double stacking procedure and were used for the determination of drug enantiomers in biosamples.},
  author       = {Pálmarsdóttir, Sveinbjörg},
  keyword      = {Analytical chemistry,Analytisk kemi},
  language     = {eng},
  pages        = {58},
  title        = {Procedures for Sample Clean-up and Concentration in Capillary Zone Electrophoresis for Determination of Drugs in Biosamples},
  year         = {1996},
}