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On-line Sample Pretreatment Based on Membrane Enrichment, Mainly Using Supported Liquid Membranes, for Chromatographic Analysis

Shen, Yin LU (1997)
Abstract (Swedish)
Popular Abstract in Swedish

I detta arbete har utvecklats automatiserade system för analys av miljöprover och av biologiska material. Två olika membranbaserade provupparbetningsmetodiker har använts för provförberedelse tillsammans med olika typer av kromatografi för själva analysen. Dessa principer har applicerats på tre olika tillämpningsområden.



Extraktion med vätskemembran har kopplats med ett automatiskt flödessystem till jonkromatografi för bestämning av flyktiga karboxylsyror i uteluft. Framför allt ättiksyra och myrsyra bestämdes i luftprover, med högre halter på landsbygden än i staden.



Ett annat helautomatiserat system har byggts upp för bestämning av kortare karboxylsyror i... (More)
Popular Abstract in Swedish

I detta arbete har utvecklats automatiserade system för analys av miljöprover och av biologiska material. Två olika membranbaserade provupparbetningsmetodiker har använts för provförberedelse tillsammans med olika typer av kromatografi för själva analysen. Dessa principer har applicerats på tre olika tillämpningsområden.



Extraktion med vätskemembran har kopplats med ett automatiskt flödessystem till jonkromatografi för bestämning av flyktiga karboxylsyror i uteluft. Framför allt ättiksyra och myrsyra bestämdes i luftprover, med högre halter på landsbygden än i staden.



Ett annat helautomatiserat system har byggts upp för bestämning av kortare karboxylsyror i markvätska. I detta system sker först en anrikning med vätskemembranextraktion följt av jonbyteskolonner för borttagande av metalljoner och oorganiska joner och slutligen jonkromatografisk slutanalys. Denna utrustning används i rutinanalys.



En utrustning för bestämning av lokalbedövningsmedel i blodplasma har konstruerats. Vätskemembranextraktion kopplad till gaskromatografi med kapillärkolonnn utnyttjas här för helautomatisk analys av ca 50 plasmaprover per dag. Detta system fungerade bra, men den tekniska lösningen för sammankopplingen var nödvändigtvis ganska komplicerad. Genom att använda en nyutvecklad extraktionsteknik, membranbaserad vätskeextraktion, har en väsentlig förenkling kunnat göras. Med denna har ett mera robust system konstruerats, som är mera lämpat för rutinanalys. (Less)
Abstract
The main objective of this work was to construct practical analytical systems for the analysis of environmental and biological samples, using membrane-based techniques for sample pretreatment and chromatography for final analysis. Two types of membrane processes have been applied, with emphasis on automation.



The supported liquid membrane (SLM) extraction was carried out in an automated continuous flow system coupled to ion-exclusion chromatography (IEC) for the determination of volatile carboxylic acids in air. Another totally automated system, using the SLM coupled on-line to anion-exchange chromatography, was developed for the determination of low-molecular weight organic acids in soil samples. Several short... (More)
The main objective of this work was to construct practical analytical systems for the analysis of environmental and biological samples, using membrane-based techniques for sample pretreatment and chromatography for final analysis. Two types of membrane processes have been applied, with emphasis on automation.



The supported liquid membrane (SLM) extraction was carried out in an automated continuous flow system coupled to ion-exclusion chromatography (IEC) for the determination of volatile carboxylic acids in air. Another totally automated system, using the SLM coupled on-line to anion-exchange chromatography, was developed for the determination of low-molecular weight organic acids in soil samples. Several short precolumns were co-ordinated with the SLM enrichment system to remove interfering compounds. The system has been used for routine measurements.



The SLM extraction was combined with capillary gas chromatography (GC) for the determination of local anaesthetics in untreated blood plasma samples. A solid phase column was located between the SLM unit and GC unit as a interface for phase switching. The system was fully automated and permitted the analysis of ca 50 samples per day.



Microporous membrane liquid-liquid extraction (MMLLE), combined with capillary GC quantitation, was presented for treating complex samples. The local anaesthetics were used as model compounds both in aqueous solutions and in blood plasma. The method can be used for routine measurements of the analytes in plasma at sub ppm level.



In this thesis, a general overview of different membranes and membrane processes, in particular SLM, and their applications of sample preptreatment are given. The applications of membrane-based sample preparation for on-line coupling with liquid chromatography and gas chromatography are discussed. Finally a brief discussion about automation of analysis is given. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof Riekkola, Marja-Liisa, Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O.Box 55, FIN-00014, University of Helsinki, Finland
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Biological Sample, Environmental Sample, Capillary Gas Chromatography, Ion Chromatography, Automation, Sample Pretreatment, Enrichment, Membrane, Extraction, Analytical chemistry, Analytisk kemi
pages
123 pages
publisher
Department of Analytical Chemistry, Lund University
defense location
Lecture Hall C, the Center for Chemistry and Chemical Engineering, Sölvegatan 39
defense date
1997-11-21 13:15
external identifiers
  • Other:ISRN: LUNKDL/(NKAK-1040)/1-123 (1997)
ISBN
91-628-2724-3
language
English
LU publication?
yes
id
44154086-1c4f-478d-b475-c881b0e3d169 (old id 29625)
date added to LUP
2007-06-13 13:07:55
date last changed
2016-09-19 08:45:15
@misc{44154086-1c4f-478d-b475-c881b0e3d169,
  abstract     = {The main objective of this work was to construct practical analytical systems for the analysis of environmental and biological samples, using membrane-based techniques for sample pretreatment and chromatography for final analysis. Two types of membrane processes have been applied, with emphasis on automation.<br/><br>
<br/><br>
The supported liquid membrane (SLM) extraction was carried out in an automated continuous flow system coupled to ion-exclusion chromatography (IEC) for the determination of volatile carboxylic acids in air. Another totally automated system, using the SLM coupled on-line to anion-exchange chromatography, was developed for the determination of low-molecular weight organic acids in soil samples. Several short precolumns were co-ordinated with the SLM enrichment system to remove interfering compounds. The system has been used for routine measurements.<br/><br>
<br/><br>
The SLM extraction was combined with capillary gas chromatography (GC) for the determination of local anaesthetics in untreated blood plasma samples. A solid phase column was located between the SLM unit and GC unit as a interface for phase switching. The system was fully automated and permitted the analysis of ca 50 samples per day.<br/><br>
<br/><br>
Microporous membrane liquid-liquid extraction (MMLLE), combined with capillary GC quantitation, was presented for treating complex samples. The local anaesthetics were used as model compounds both in aqueous solutions and in blood plasma. The method can be used for routine measurements of the analytes in plasma at sub ppm level.<br/><br>
<br/><br>
In this thesis, a general overview of different membranes and membrane processes, in particular SLM, and their applications of sample preptreatment are given. The applications of membrane-based sample preparation for on-line coupling with liquid chromatography and gas chromatography are discussed. Finally a brief discussion about automation of analysis is given.},
  author       = {Shen, Yin},
  isbn         = {91-628-2724-3},
  keyword      = {Biological Sample,Environmental Sample,Capillary Gas Chromatography,Ion Chromatography,Automation,Sample Pretreatment,Enrichment,Membrane,Extraction,Analytical chemistry,Analytisk kemi},
  language     = {eng},
  pages        = {123},
  publisher    = {ARRAY(0xa148438)},
  title        = {On-line Sample Pretreatment Based on Membrane Enrichment, Mainly Using Supported Liquid Membranes, for Chromatographic Analysis},
  year         = {1997},
}