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Recombinant human insulin-like growth factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon

Abrahamsson, S. O. LU ; Lundborg, G. LU and Lohmander, L. S. (1991) In Journal of Orthopaedic Research 9(4). p.495-502
Abstract

Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the... (More)

Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The E(max) of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the E(max) of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Orthopaedic Research
volume
9
issue
4
pages
8 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0026198752
ISSN
0736-0266
language
English
LU publication?
no
id
29b86909-700a-46b1-8e80-13cafb6dba4b
date added to LUP
2016-05-04 18:15:44
date last changed
2016-07-07 14:37:48
@misc{29b86909-700a-46b1-8e80-13cafb6dba4b,
  abstract     = {<p>Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The E(max) of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the E(max) of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.</p>},
  author       = {Abrahamsson, S. O. and Lundborg, G. and Lohmander, L. S.},
  issn         = {0736-0266},
  language     = {eng},
  number       = {4},
  pages        = {495--502},
  publisher    = {ARRAY(0x8dd6498)},
  series       = {Journal of Orthopaedic Research},
  title        = {Recombinant human insulin-like growth factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon},
  volume       = {9},
  year         = {1991},
}