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In vivo activation of dendritic cells and T cells during Salmonella enterica serovar Typhimurium infection

Yrlid, U LU ; Svensson, Mattias LU ; Håkansson, Anders P LU ; Chambers, B J; Ljunggren, H G and Wick, M J LU (2001) In Infection and Immunity 69(9). p.35-5726
Abstract

The present study was initiated to gain insight into the interaction between splenic dendritic cells (DC) and Salmonella enterica serovar Typhimurium in vivo. Splenic phagocytic cell populations associated with green fluorescent protein (GFP)-expressing bacteria and the bacterium-specific T-cell response were evaluated in mice given S. enterica serovar Typhimurium expressing GFP and ovalbumin. Flow cytometry analysis revealed that GFP-positive splenic DC (CD11c+ major histocompatibility complex class II-positive [MHC-II+] cells) were present following bacterial administration, and confocal microscopy showed that GFP-expressing bacteria were contained within CD11c+ MHC-II+ splenocytes. Furthermore, splenic DC and T cells were activated... (More)

The present study was initiated to gain insight into the interaction between splenic dendritic cells (DC) and Salmonella enterica serovar Typhimurium in vivo. Splenic phagocytic cell populations associated with green fluorescent protein (GFP)-expressing bacteria and the bacterium-specific T-cell response were evaluated in mice given S. enterica serovar Typhimurium expressing GFP and ovalbumin. Flow cytometry analysis revealed that GFP-positive splenic DC (CD11c+ major histocompatibility complex class II-positive [MHC-II+] cells) were present following bacterial administration, and confocal microscopy showed that GFP-expressing bacteria were contained within CD11c+ MHC-II+ splenocytes. Furthermore, splenic DC and T cells were activated following Salmonella infection. This was shown by increased surface expression of CD86 and CD40 on CD11c+ MHC-II+ cells and increased CD44 and CD69 expression on CD4+ and CD8+ T cells. Salmonella-specific gamma interferon (IFN-gamma)-producing cells in both of these T-cell subsets, as well as cytolytic effector cells, were also generated in mice given live bacteria. The frequency of Salmonella-specific CD4+ T cells producing IFN-gamma was greater than that of specific CD8+ T cells producing IFN-gamma in the same infected animal. This supports the argument that the predominant source of IFN-gamma production by cells of the specific immune response is CD4+ T cells. Finally, DC that phagocytosed live or heat-killed Salmonella in vitro primed bacterium-specific IFN-gamma-producing CD4+ and CD8+ T cells as well as cytolytic effector cells following administration into naïve mice. Together these data suggest that DC are involved in priming naïve T cells to Salmonella in vivo.

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author
organization
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type
Contribution to journal
publication status
published
keywords
Animals, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cells, Cultured, Dendritic Cells, Green Fluorescent Proteins, Interferon-gamma, Luminescent Proteins, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Ovalbumin, Phagocytes, Salmonella Infections, Animal, Salmonella typhimurium, Spleen
in
Infection and Immunity
volume
69
issue
9
pages
10 pages
publisher
American Society for Microbiology
external identifiers
  • Scopus:0034741924
ISSN
0019-9567
DOI
10.1128/IAI.69.9.5726-5735.2001
language
English
LU publication?
yes
id
2bf9f9e4-af31-42ed-a9ae-81ea79e09fd6
date added to LUP
2016-05-21 13:55:25
date last changed
2016-12-04 04:51:50
@misc{2bf9f9e4-af31-42ed-a9ae-81ea79e09fd6,
  abstract     = {<p>The present study was initiated to gain insight into the interaction between splenic dendritic cells (DC) and Salmonella enterica serovar Typhimurium in vivo. Splenic phagocytic cell populations associated with green fluorescent protein (GFP)-expressing bacteria and the bacterium-specific T-cell response were evaluated in mice given S. enterica serovar Typhimurium expressing GFP and ovalbumin. Flow cytometry analysis revealed that GFP-positive splenic DC (CD11c+ major histocompatibility complex class II-positive [MHC-II+] cells) were present following bacterial administration, and confocal microscopy showed that GFP-expressing bacteria were contained within CD11c+ MHC-II+ splenocytes. Furthermore, splenic DC and T cells were activated following Salmonella infection. This was shown by increased surface expression of CD86 and CD40 on CD11c+ MHC-II+ cells and increased CD44 and CD69 expression on CD4+ and CD8+ T cells. Salmonella-specific gamma interferon (IFN-gamma)-producing cells in both of these T-cell subsets, as well as cytolytic effector cells, were also generated in mice given live bacteria. The frequency of Salmonella-specific CD4+ T cells producing IFN-gamma was greater than that of specific CD8+ T cells producing IFN-gamma in the same infected animal. This supports the argument that the predominant source of IFN-gamma production by cells of the specific immune response is CD4+ T cells. Finally, DC that phagocytosed live or heat-killed Salmonella in vitro primed bacterium-specific IFN-gamma-producing CD4+ and CD8+ T cells as well as cytolytic effector cells following administration into naïve mice. Together these data suggest that DC are involved in priming naïve T cells to Salmonella in vivo.</p>},
  author       = {Yrlid, U and Svensson, Mattias and Håkansson, Anders P and Chambers, B J and Ljunggren, H G and Wick, M J},
  issn         = {0019-9567},
  keyword      = {Animals,CD4-Positive T-Lymphocytes,CD8-Positive T-Lymphocytes,Cells, Cultured,Dendritic Cells,Green Fluorescent Proteins,Interferon-gamma,Luminescent Proteins,Lymphocyte Activation,Mice,Mice, Inbred C57BL,Ovalbumin,Phagocytes,Salmonella Infections, Animal,Salmonella typhimurium,Spleen},
  language     = {eng},
  number       = {9},
  pages        = {35--5726},
  publisher    = {ARRAY(0x96acae0)},
  series       = {Infection and Immunity},
  title        = {In vivo activation of dendritic cells and T cells during Salmonella enterica serovar Typhimurium infection},
  url          = {http://dx.doi.org/10.1128/IAI.69.9.5726-5735.2001 },
  volume       = {69},
  year         = {2001},
}