Advanced

A fast and sensitive method for the separation of carotenoids using ultra-high performance supercritical fluid chromatography-mass spectrometry

Jumaah, Firas LU ; Plaza, Merichel LU ; Abrahamsson, Victor LU ; Turner, Charlotta LU and Sandahl, Margareta LU (2016) In Analytical and Bioanalytical Chemistry
Abstract

In this study, a rapid and sensitive ultra-high performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS) method has been developed and partially validated for the separation of carotenoids within less than 6 min. Six columns of orthogonal selectivity were examined, and the best separation was obtained by using a 1-aminoanthracene (1-AA) column. The length of polyene chain as well as the number of hydroxyl groups in the structure of the studied carotenoids determines their differences in the physiochemical properties and thus the separation that is achieved on this column. All of the investigated carotenoids were baseline separated with resolution values greater than 1.5. The effects of gradient program, back... (More)

In this study, a rapid and sensitive ultra-high performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS) method has been developed and partially validated for the separation of carotenoids within less than 6 min. Six columns of orthogonal selectivity were examined, and the best separation was obtained by using a 1-aminoanthracene (1-AA) column. The length of polyene chain as well as the number of hydroxyl groups in the structure of the studied carotenoids determines their differences in the physiochemical properties and thus the separation that is achieved on this column. All of the investigated carotenoids were baseline separated with resolution values greater than 1.5. The effects of gradient program, back pressure, and column temperature were studied with respect to chromatographic properties such as retention and selectivity. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) were compared in both positive and negative mode, using both direct infusion and hyphenated with UHPSFC. The ESI in positive mode provided the highest response. The coefficient of determination (R2) for all calibration curves were greater than 0.998. Limit of detection (LOD) was in the range of 2.6 and 25.2 ng/mL for α-carotene and astaxanthin, respectively, whereas limit of quantification (LOQ) was in the range of 7.8 and 58.0 ng/mL for α-carotene and astaxanthin, respectively. Repeatability and intermediate precision of the developed UHPSFC-MS method were determined and found to be RSD <3 % and RSD <6 %, respectively. The method was applied in order to determine carotenoids in supercritical fluid extracts of microalgae and rosehip. [Figure not available: see fulltext.]

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Carotene, Mass spectrometry, Method development, Orthogonal column screening, Supercritical fluid, Xanthophyll
in
Analytical and Bioanalytical Chemistry
pages
12 pages
publisher
Springer
external identifiers
  • Scopus:84976286261
ISSN
1618-2642
DOI
10.1007/s00216-016-9707-5
language
English
LU publication?
yes
id
33017a89-988d-42ed-b993-eeee1f773486
date added to LUP
2016-07-26 09:54:12
date last changed
2016-09-20 03:06:57
@misc{33017a89-988d-42ed-b993-eeee1f773486,
  abstract     = {<p>In this study, a rapid and sensitive ultra-high performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS) method has been developed and partially validated for the separation of carotenoids within less than 6 min. Six columns of orthogonal selectivity were examined, and the best separation was obtained by using a 1-aminoanthracene (1-AA) column. The length of polyene chain as well as the number of hydroxyl groups in the structure of the studied carotenoids determines their differences in the physiochemical properties and thus the separation that is achieved on this column. All of the investigated carotenoids were baseline separated with resolution values greater than 1.5. The effects of gradient program, back pressure, and column temperature were studied with respect to chromatographic properties such as retention and selectivity. Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) were compared in both positive and negative mode, using both direct infusion and hyphenated with UHPSFC. The ESI in positive mode provided the highest response. The coefficient of determination (R<sup>2</sup>) for all calibration curves were greater than 0.998. Limit of detection (LOD) was in the range of 2.6 and 25.2 ng/mL for α-carotene and astaxanthin, respectively, whereas limit of quantification (LOQ) was in the range of 7.8 and 58.0 ng/mL for α-carotene and astaxanthin, respectively. Repeatability and intermediate precision of the developed UHPSFC-MS method were determined and found to be RSD &lt;3 % and RSD &lt;6 %, respectively. The method was applied in order to determine carotenoids in supercritical fluid extracts of microalgae and rosehip. [Figure not available: see fulltext.]</p>},
  author       = {Jumaah, Firas and Plaza, Merichel and Abrahamsson, Victor and Turner, Charlotta and Sandahl, Margareta},
  issn         = {1618-2642},
  keyword      = {Carotene,Mass spectrometry,Method development,Orthogonal column screening,Supercritical fluid,Xanthophyll},
  language     = {eng},
  month        = {06},
  pages        = {12},
  publisher    = {ARRAY(0x8794cb0)},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {A fast and sensitive method for the separation of carotenoids using ultra-high performance supercritical fluid chromatography-mass spectrometry},
  url          = {http://dx.doi.org/10.1007/s00216-016-9707-5},
  year         = {2016},
}