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Ethanol production from steam pretreated sugarcane bagasse via simultaneous saccharification and fermentation (SSF) using cellulase and b.glucosidase enzymes

Baudel, Henrique; Sendelius, Johan; Carrasco, Cristhian LU ; Roslander, Christian LU and Galbe, Mats LU (2008) In XVII Congreso Brasileiro de Engenharia Quimica p.1-8
Abstract
Cellulosic ethanol was produced via simultaneous enzymatic hydrolysis and fermentation of steam pretreated sugarcane bagasse. Sugarcane bagasse underwent steam pretreatments at Log Ro 3.05-4.09, under catalysed (SO2 2% and H2SO4 0.25%) and uncatalysed conditions. Digestibilities of the steam pretreated bagasse were assessed via enzymatic hydrolysis using cellulase (Celluclast 1.5L) and B-glucosidase (Novozym 188) enzyme formulations at 2% WIS, 40 ºC, pH 4.8, 72h under continuous stirring (200 rpm). SO2-catalysed steam pretreated bagasses produced at moderate severities (180 or 190 ºC, 5 minutes) presented digestibilities up to 82%. Hemicellulose removal influenced pretreatment efficiency rather than delignification. SO2-steam pretreated... (More)
Cellulosic ethanol was produced via simultaneous enzymatic hydrolysis and fermentation of steam pretreated sugarcane bagasse. Sugarcane bagasse underwent steam pretreatments at Log Ro 3.05-4.09, under catalysed (SO2 2% and H2SO4 0.25%) and uncatalysed conditions. Digestibilities of the steam pretreated bagasse were assessed via enzymatic hydrolysis using cellulase (Celluclast 1.5L) and B-glucosidase (Novozym 188) enzyme formulations at 2% WIS, 40 ºC, pH 4.8, 72h under continuous stirring (200 rpm). SO2-catalysed steam pretreated bagasses produced at moderate severities (180 or 190 ºC, 5 minutes) presented digestibilities up to 82%. Hemicellulose removal influenced pretreatment efficiency rather than delignification. SO2-steam pretreated bagasses were processed by SSF (simultaneous saccharification and fermentation) at 5% WIS loading. Yields of 0.40-0.41 g ethanol/g glucose (approx. 80% of the theoretical) and high selectivity through ethyl alcohol were obtained for all investigated conditions, leading to a potential production of 230 L ethanol/ ton dry bagasse. (Less)
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organization
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Chapter in Book/Report/Conference proceeding
publication status
in press
subject
keywords
simultaneous saccharification and fermentation (SSF), steam pretreatment, ethanol, Enzymatic hydrolysis, sugarcane bagasse
in
XVII Congreso Brasileiro de Engenharia Quimica
pages
1 - 8
publisher
XVII Congreso Brasileiro de Engenharia Quimica
language
English
LU publication?
yes
id
773c80b3-0fda-4ced-993e-0b15892eb978 (old id 3412389)
date added to LUP
2013-02-20 09:19:30
date last changed
2016-04-16 08:13:53
@misc{773c80b3-0fda-4ced-993e-0b15892eb978,
  abstract     = {Cellulosic ethanol was produced via simultaneous enzymatic hydrolysis and fermentation of steam pretreated sugarcane bagasse. Sugarcane bagasse underwent steam pretreatments at Log Ro 3.05-4.09, under catalysed (SO2 2% and H2SO4 0.25%) and uncatalysed conditions. Digestibilities of the steam pretreated bagasse were assessed via enzymatic hydrolysis using cellulase (Celluclast 1.5L) and B-glucosidase (Novozym 188) enzyme formulations at 2% WIS, 40 ºC, pH 4.8, 72h under continuous stirring (200 rpm). SO2-catalysed steam pretreated bagasses produced at moderate severities (180 or 190 ºC, 5 minutes) presented digestibilities up to 82%. Hemicellulose removal influenced pretreatment efficiency rather than delignification. SO2-steam pretreated bagasses were processed by SSF (simultaneous saccharification and fermentation) at 5% WIS loading. Yields of 0.40-0.41 g ethanol/g glucose (approx. 80% of the theoretical) and high selectivity through ethyl alcohol were obtained for all investigated conditions, leading to a potential production of 230 L ethanol/ ton dry bagasse.},
  author       = {Baudel, Henrique and Sendelius, Johan and Carrasco, Cristhian and Roslander, Christian and Galbe, Mats},
  keyword      = {simultaneous saccharification and fermentation (SSF),steam pretreatment,ethanol,Enzymatic hydrolysis,sugarcane bagasse},
  language     = {eng},
  pages        = {1--8},
  publisher    = {ARRAY(0x9445088)},
  series       = {XVII Congreso Brasileiro de Engenharia Quimica},
  title        = {Ethanol production from steam pretreated sugarcane bagasse via simultaneous saccharification and fermentation (SSF) using cellulase and b.glucosidase enzymes},
  year         = {2008},
}