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Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis

Végvári, Ákos LU and Hjertén, Stellan (2002) In Journal of Chromatography A 960(1-2). p.221-227
Abstract
A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary electrophoresis, DNA, Polyacrylamide, Allyl-b-cyclodextrin
in
Journal of Chromatography A
volume
960
issue
1-2
pages
221 - 227
publisher
Elsevier
external identifiers
  • Scopus:0037172696
ISSN
0021-9673
DOI
10.1016/S0021-9673(01)01436-4,
language
English
LU publication?
no
id
cc510027-4876-49d2-a341-fdf6e796c72d (old id 3516236)
date added to LUP
2013-02-25 10:39:47
date last changed
2016-10-13 04:27:52
@misc{cc510027-4876-49d2-a341-fdf6e796c72d,
  abstract     = {A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.},
  author       = {Végvári, Ákos and Hjertén, Stellan},
  issn         = {0021-9673},
  keyword      = {Capillary electrophoresis,DNA,Polyacrylamide,Allyl-b-cyclodextrin},
  language     = {eng},
  number       = {1-2},
  pages        = {221--227},
  publisher    = {ARRAY(0xa699a18)},
  series       = {Journal of Chromatography A},
  title        = {Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis},
  url          = {http://dx.doi.org/10.1016/S0021-9673(01)01436-4,},
  volume       = {960},
  year         = {2002},
}