Immunological systems biology: gene expression analysis of B-cell development in Ramos B-cells.
Ollila, Juha and Vihinen, Mauno LU
(2007)
In Molecular Immunology
44(14).
p.3537-3551
- Abstract
- B-cell development into antibody producing cells is a complex process that relies on the tightly controlled production of hundreds of genes and proteins. A B-cell is activated through the B-cell receptor (BCR) and this activation is modified by different co-stimulatory or inhibitory co-receptors. The concerted action of signals from BCR and from co-receptors decides the fate of the B-cells. The majority of B-cells enter apoptosis, while some of them progress through the cell cycle and become, for example, antibody producing plasma cells. We studied BCR stimulated Ramos B-cells to explore the expression of BCR pathway, cell cycle and apoptosis related genes. We followed, using microarrays, the gene expression for several days after BCR... (More)
- B-cell development into antibody producing cells is a complex process that relies on the tightly controlled production of hundreds of genes and proteins. A B-cell is activated through the B-cell receptor (BCR) and this activation is modified by different co-stimulatory or inhibitory co-receptors. The concerted action of signals from BCR and from co-receptors decides the fate of the B-cells. The majority of B-cells enter apoptosis, while some of them progress through the cell cycle and become, for example, antibody producing plasma cells. We studied BCR stimulated Ramos B-cells to explore the expression of BCR pathway, cell cycle and apoptosis related genes. We followed, using microarrays, the gene expression for several days after BCR engagement. Several bioinformatics methods were used to investigate the properties and common features of co-expressed genes. Certain gene ontologies have statistically significant enrichment into clusters of similarly expressed genes. The cell signaling pathways and gene expression data were combined to reveal detailed information about biological processes and B-cell systems biology. The results provide knowledge of the development of adaptive immunity and clues about how the pathways are affected by regulation of the expression of genes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3635275
- author
- Ollila, Juha
and Vihinen, Mauno
LU
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Receptors, Immune System: immunology, Cell Cycle: genetics, Apoptosis: genetics, B-Lymphocytes: immunology, Antigen, B-Cell: genetics, Signal Transduction: genetics
- in
- Molecular Immunology
- volume
- 44
- issue
- 14
- pages
- 3537 - 3551
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- pmid:17485117
- scopus:34248523993
- ISSN
- 1872-9142
- DOI
- 10.1016/j.molimm.2007.03.009
- language
- English
- LU publication?
- no
- id
- 528a7580-12da-466e-a1f1-cc2951ba8a20 (old id 3635275)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/17485117?dopt=Abstract
- date added to LUP
- 2016-04-04 09:05:58
- date last changed
- 2022-03-23 03:57:21
@article{528a7580-12da-466e-a1f1-cc2951ba8a20,
abstract = {{B-cell development into antibody producing cells is a complex process that relies on the tightly controlled production of hundreds of genes and proteins. A B-cell is activated through the B-cell receptor (BCR) and this activation is modified by different co-stimulatory or inhibitory co-receptors. The concerted action of signals from BCR and from co-receptors decides the fate of the B-cells. The majority of B-cells enter apoptosis, while some of them progress through the cell cycle and become, for example, antibody producing plasma cells. We studied BCR stimulated Ramos B-cells to explore the expression of BCR pathway, cell cycle and apoptosis related genes. We followed, using microarrays, the gene expression for several days after BCR engagement. Several bioinformatics methods were used to investigate the properties and common features of co-expressed genes. Certain gene ontologies have statistically significant enrichment into clusters of similarly expressed genes. The cell signaling pathways and gene expression data were combined to reveal detailed information about biological processes and B-cell systems biology. The results provide knowledge of the development of adaptive immunity and clues about how the pathways are affected by regulation of the expression of genes.}},
author = {{Ollila, Juha and Vihinen, Mauno}},
issn = {{1872-9142}},
keywords = {{Receptors; Immune System: immunology; Cell Cycle: genetics; Apoptosis: genetics; B-Lymphocytes: immunology; Antigen; B-Cell: genetics; Signal Transduction: genetics}},
language = {{eng}},
number = {{14}},
pages = {{3537--3551}},
publisher = {{Pergamon Press Ltd.}},
series = {{Molecular Immunology}},
title = {{Immunological systems biology: gene expression analysis of B-cell development in Ramos B-cells.}},
url = {{http://dx.doi.org/10.1016/j.molimm.2007.03.009}},
doi = {{10.1016/j.molimm.2007.03.009}},
volume = {{44}},
year = {{2007}},
}