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Amperometric Carbon Paste Biosensors Based on Horseradish Peroxidase and Oxidases

Rajendran, Vijayakumar LU (1999)
Abstract
Amperometric carbon paste peroxide sensors based on horseradish peroxidase (HRP) were developed. In the first approach, HRP was chemically modified with polyethylene glycol to improve the stability of the enzyme in the organic paste matrix and compared with native HRP electrode. In the second approach, HRP was "wired" with a redox polymer containing a covalently bound phenoxazine type mediator, toluidine blue O (TBO), acting as a two-electron donor. Here comparison was made between mediated vs. non-mediated electron transfer and also the type of the paste binders, paraffin oil vs. paraffin wax. The peroxide sensor based on TBO was subsequently miniaturised and integrated with a glucose oxidase immobilised micro reactor in a micro flow cell... (More)
Amperometric carbon paste peroxide sensors based on horseradish peroxidase (HRP) were developed. In the first approach, HRP was chemically modified with polyethylene glycol to improve the stability of the enzyme in the organic paste matrix and compared with native HRP electrode. In the second approach, HRP was "wired" with a redox polymer containing a covalently bound phenoxazine type mediator, toluidine blue O (TBO), acting as a two-electron donor. Here comparison was made between mediated vs. non-mediated electron transfer and also the type of the paste binders, paraffin oil vs. paraffin wax. The peroxide sensor based on TBO was subsequently miniaturised and integrated with a glucose oxidase immobilised micro reactor in a micro flow cell for the analysis of glucose. Amperometric carbon paste biosensors based on oxidases (alcohol oxidase, glucose oxidase and L-lactate oxidase) co-immobilised with HRP were developed. The biosensors were designed based on two different approaches, i.e. direct or mediated electron transfer. For non-mediated systems, the enzymes were admixed with both additives previously shown to be efficient and also some new activators/stabilisers, such as polyethylenimine, lactitol, DEAE-dextran, polymeric dialdehyde, gluteraldehyde and a polyester sulfonic acid cation exchanger (Eastman AQ-29D) which was either mixed in the paste or applied as a protective membrane on the electrode surface. The mediated alcohol biosensor was based on an osmium containing three-dimensional redox hydrogel, (poly[1-vinyl imidazole osmium (4,4´-dimethyl-bipyridine)2Cl])+/2+, which was used to "wire" the HRP before co-immobilising the alcohol oxidase. The sensors were characterised regarding the sensitivity, selectivity, pH optima, operational and storage stabilities in a flow injection mode at a working potential of -50 mV vs. Ag/AgCl (0.1 M KCl). The developed sensors were applied under real operating conditions, such as, the alcohol sensor was used as a detection unit in column liquid chromatography for the determination of ethanol and methanol in biological fluids, while the L-lactate and glucose sensors were housed in a dual flow-through cell for the simultaneous monitoring of glucose and L-lactic acid during a fermentation process in an aqueous two-phase system by on-line FIA with microdialysis sampling. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Prof Dominquez, Elena, Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcala, E-288 71 Alcala de Henares, Madrid, Spain.
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Analytical chemistry, on-line monitoring, l-lactate, glucose, ethanol, Biosensor, Carbon paste, Analytisk kemi
pages
150 pages
publisher
Department of Analytical Chemistry, Lund University
defense location
Chemical center, Sölvegatan 39, Lund.
defense date
1999-01-15 10:15:00
external identifiers
  • other:ISRN: LUNKDL/NKAK--1998/1046
language
English
LU publication?
yes
additional info
Article: Comparison of carbon paste electrodes modified with native andpolyethylene glycol derivatised horseradish peroxidase for the amperometric monitoring of H2O2A. Vijayakumar, Elisabeth Csöregi, Tautgirdas Ruzgas and Lo GortonSensors and Actuators B 37 (1996) 97-102 Article: Amperometric peroxide sensor based on horseradish peroxidase and toluidine blue O-acrylamide polymer in carbon pasteVijay Rajendran, Elisabeth Csöregi, Yoshi Okamoto and Lo GortonAnalytica Chimica Acta 373 (1998) 241-251 Article: Integrated micro flow cell for glucose analysisVijay Rajendran, Johan Drott, Elisabeth Csöregi, Lo Gorton & Thomas LaurellManuscript Article: Simultaneous monitoring of glucose and L-lactic acid during afermentation process in an aqueous two-phase system by on-line FIA with microdialysis sampling and dual biosensor detectionRong Wei Min, Vijay Rajendran, Niklas Larsson, Lo Gorton, Jordi Planas and Bärbel Hahn-HägerdalAnalytica Chimica Acta 366 (1998) 127-135 Article: Rapid alcohol determination in plasma and urine by column liquid chromatography with biosensor detectionHelena Lidén, A.R. Vijayakumar, Lo Gorton and György Marko-VargaJournal of Pharmaceutical and Biomedical Analysis, 17 (1998) 111-128. Article: Alcohol biosensors based on coupled oxidase-peroxidase systems A.R. Vijayakumar, Elisabeth Csöregi, Adam Heller and Lo GortonAnalytica Chimica Acta 327 (1996) 223-234 The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
ba151697-7dfd-4f19-8139-c21decd4ce1c (old id 39292)
date added to LUP
2016-04-04 12:24:24
date last changed
2018-11-21 21:10:46
@phdthesis{ba151697-7dfd-4f19-8139-c21decd4ce1c,
  abstract     = {{Amperometric carbon paste peroxide sensors based on horseradish peroxidase (HRP) were developed. In the first approach, HRP was chemically modified with polyethylene glycol to improve the stability of the enzyme in the organic paste matrix and compared with native HRP electrode. In the second approach, HRP was "wired" with a redox polymer containing a covalently bound phenoxazine type mediator, toluidine blue O (TBO), acting as a two-electron donor. Here comparison was made between mediated vs. non-mediated electron transfer and also the type of the paste binders, paraffin oil vs. paraffin wax. The peroxide sensor based on TBO was subsequently miniaturised and integrated with a glucose oxidase immobilised micro reactor in a micro flow cell for the analysis of glucose. Amperometric carbon paste biosensors based on oxidases (alcohol oxidase, glucose oxidase and L-lactate oxidase) co-immobilised with HRP were developed. The biosensors were designed based on two different approaches, i.e. direct or mediated electron transfer. For non-mediated systems, the enzymes were admixed with both additives previously shown to be efficient and also some new activators/stabilisers, such as polyethylenimine, lactitol, DEAE-dextran, polymeric dialdehyde, gluteraldehyde and a polyester sulfonic acid cation exchanger (Eastman AQ-29D) which was either mixed in the paste or applied as a protective membrane on the electrode surface. The mediated alcohol biosensor was based on an osmium containing three-dimensional redox hydrogel, (poly[1-vinyl imidazole osmium (4,4´-dimethyl-bipyridine)2Cl])+/2+, which was used to "wire" the HRP before co-immobilising the alcohol oxidase. The sensors were characterised regarding the sensitivity, selectivity, pH optima, operational and storage stabilities in a flow injection mode at a working potential of -50 mV vs. Ag/AgCl (0.1 M KCl). The developed sensors were applied under real operating conditions, such as, the alcohol sensor was used as a detection unit in column liquid chromatography for the determination of ethanol and methanol in biological fluids, while the L-lactate and glucose sensors were housed in a dual flow-through cell for the simultaneous monitoring of glucose and L-lactic acid during a fermentation process in an aqueous two-phase system by on-line FIA with microdialysis sampling.}},
  author       = {{Rajendran, Vijayakumar}},
  keywords     = {{Analytical chemistry; on-line monitoring; l-lactate; glucose; ethanol; Biosensor; Carbon paste; Analytisk kemi}},
  language     = {{eng}},
  publisher    = {{Department of Analytical Chemistry, Lund University}},
  school       = {{Lund University}},
  title        = {{Amperometric Carbon Paste Biosensors Based on Horseradish Peroxidase and Oxidases}},
  year         = {{1999}},
}