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Epstein-Barr virus binding to virus-carrying cell lines is enhanced in the presence of C3 and C3d

Wells, A.; Koide, N.; Eggertsen, G.; Lundwall, Åke LU ; Godal, T.; Steen, H. B. and Klein, G. (1984) In J Gen Virol 65 ( Pt 3). p.13-507
Abstract
The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are... (More)
The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are distinct, although they both involve the same cell surface complex. The two receptors are believed to display cooperativity. (Less)
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published
subject
keywords
Complement/*analysis, Receptors, Kinetics, Humans, Human/*metabolism, Herpesvirus 4, Complement C3d, Cell Line, Complement C3/*metabolism, Virus/*analysis, Research Support, Non-U.S. Gov't, U.S. Gov't, P.H.S.
in
J Gen Virol
volume
65 ( Pt 3)
pages
13 - 507
external identifiers
  • Scopus:0021355720
language
English
LU publication?
no
id
e2c03fa6-0d1e-4b7f-bfbd-ade9ae01db80 (old id 3965074)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6321638
date added to LUP
2013-08-11 14:41:10
date last changed
2016-06-29 09:03:24
@misc{e2c03fa6-0d1e-4b7f-bfbd-ade9ae01db80,
  abstract     = {The relationship between the receptors for the Epstein-Barr virus (EBV) and the C3d fragment of complement was investigated at the molecular level. In the presence of cell-bound C3, virus binding was enhanced in EBV genome-carrying lines. An identical effect could be elicited by C3d at one-quarter the weight amount; C3b and methylamine-treated C3 had no effect on virus binding. The minimum concentration of C3 which produced significant enhancement was 25 micrograms/ml. Virus binding increases were observed only after 20 min of complement-cell co-incubation. The response was not noted with EBV-negative lines and was independent of virus strain assayed (B95-8 and P3HR-1). These studies suggest that the binding sites for the two moieties are distinct, although they both involve the same cell surface complex. The two receptors are believed to display cooperativity.},
  author       = {Wells, A. and Koide, N. and Eggertsen, G. and Lundwall, Åke and Godal, T. and Steen, H. B. and Klein, G.},
  keyword      = {Complement/*analysis,Receptors,Kinetics,Humans,Human/*metabolism,Herpesvirus 4,Complement C3d,Cell Line,Complement C3/*metabolism,Virus/*analysis,Research Support,Non-U.S. Gov't,U.S. Gov't,P.H.S.},
  language     = {eng},
  pages        = {13--507},
  series       = {J Gen Virol},
  title        = {Epstein-Barr virus binding to virus-carrying cell lines is enhanced in the presence of C3 and C3d},
  volume       = {65 ( Pt 3)},
  year         = {1984},
}