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Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain

Wetsel, R. A.; Lundwall, Åke LU ; Davidson, F.; Gibson, T.; Tack, B. F. and Fey, G. H. (1984) In J Biol Chem 259(22). p.62-13857
Abstract
From the inserts of two recombinant plasmids isolated from a murine liver cDNA library, the nucleotide sequence coding for the C3 alpha chain was obtained and the corresponding amino acid sequence was derived. The alpha chain portion of the mRNA is 2979 nucleotides long, specifying a polypeptide of 993 amino acids. The molecular weight of the alpha chain, in the absence of carbohydrate, was calculated from the sequence data to be 112,933. Two possible carbohydrate attachment sites were predicted at residues 269 (Asn) and 947 (Asn). In addition, the positions for two putative factor I cleavage sites were predicted from a comparison with the cleavage sites in the human C3 alpha chain. The C3 alpha chain contains 24 cysteine residues, 10 of... (More)
From the inserts of two recombinant plasmids isolated from a murine liver cDNA library, the nucleotide sequence coding for the C3 alpha chain was obtained and the corresponding amino acid sequence was derived. The alpha chain portion of the mRNA is 2979 nucleotides long, specifying a polypeptide of 993 amino acids. The molecular weight of the alpha chain, in the absence of carbohydrate, was calculated from the sequence data to be 112,933. Two possible carbohydrate attachment sites were predicted at residues 269 (Asn) and 947 (Asn). In addition, the positions for two putative factor I cleavage sites were predicted from a comparison with the cleavage sites in the human C3 alpha chain. The C3 alpha chain contains 24 cysteine residues, 10 of these clustered in the C-terminal 175 amino acids of the alpha chain. Together with the accompanying report (Lundwall, A, Wetsel, R.A., Domdey, H., Tack, B.F., and Fey, G.H. (1984) J. Biol. Chem. 259, 13851-13856), this study completes the nucleotide and amino acid structure of the murine precursor prepro-C3 molecule. (Less)
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published
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keywords
Liver/analysis, DNA Restriction Enzymes/metabolism, DNA/*analysis, Complement C3/*genetics, Codon/analysis, Molecular, Cloning, Base Sequence, Amino Acid Sequence, Animals, Mice, Molecular Weight, Research Support, Non-U.S. Gov't, U.S. Gov't, P.H.S.
in
J Biol Chem
volume
259
issue
22
pages
62 - 13857
language
English
LU publication?
no
id
b625fa96-2fe4-48b8-a890-37cbd3f37390 (old id 3965083)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6094532
date added to LUP
2013-08-11 18:59:06
date last changed
2016-06-29 09:03:11
@misc{b625fa96-2fe4-48b8-a890-37cbd3f37390,
  abstract     = {From the inserts of two recombinant plasmids isolated from a murine liver cDNA library, the nucleotide sequence coding for the C3 alpha chain was obtained and the corresponding amino acid sequence was derived. The alpha chain portion of the mRNA is 2979 nucleotides long, specifying a polypeptide of 993 amino acids. The molecular weight of the alpha chain, in the absence of carbohydrate, was calculated from the sequence data to be 112,933. Two possible carbohydrate attachment sites were predicted at residues 269 (Asn) and 947 (Asn). In addition, the positions for two putative factor I cleavage sites were predicted from a comparison with the cleavage sites in the human C3 alpha chain. The C3 alpha chain contains 24 cysteine residues, 10 of these clustered in the C-terminal 175 amino acids of the alpha chain. Together with the accompanying report (Lundwall, A, Wetsel, R.A., Domdey, H., Tack, B.F., and Fey, G.H. (1984) J. Biol. Chem. 259, 13851-13856), this study completes the nucleotide and amino acid structure of the murine precursor prepro-C3 molecule.},
  author       = {Wetsel, R. A. and Lundwall, Åke and Davidson, F. and Gibson, T. and Tack, B. F. and Fey, G. H.},
  keyword      = {Liver/analysis,DNA Restriction Enzymes/metabolism,DNA/*analysis,Complement C3/*genetics,Codon/analysis,Molecular,Cloning,Base Sequence,Amino Acid Sequence,Animals,Mice,Molecular Weight,Research Support,Non-U.S. Gov't,U.S. Gov't,P.H.S.},
  language     = {eng},
  number       = {22},
  pages        = {62--13857},
  series       = {J Biol Chem},
  title        = {Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the alpha chain},
  volume       = {259},
  year         = {1984},
}