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Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation

Lundwall, Åke LU ; Dackowski, W.; Cohen, E.; Shaffer, M.; Mahr, A.; Dahlback, B.; Stenflo, J. and Wydro, R. (1986) In Proc Natl Acad Sci U S A 83(18). p.20-6716
Abstract
Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to... (More)
Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases. (Less)
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organization
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Contribution to journal
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published
subject
keywords
Protein S, Humans, Glycoproteins/analysis/*genetics, Epidermal Growth Factor/analysis, DNA/*analysis/isolation & purification, Amino Acid Sequence, Base Sequence, RNA, Messenger/analysis, Research Support, Non-U.S. Gov't
in
Proc Natl Acad Sci U S A
volume
83
issue
18
pages
20 - 6716
external identifiers
  • Scopus:0010336865
language
English
LU publication?
yes
id
1a06e725-e06e-4573-97eb-3c51a29c7f54 (old id 3965135)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2944113
date added to LUP
2013-08-11 18:30:50
date last changed
2016-04-16 12:22:08
@misc{1a06e725-e06e-4573-97eb-3c51a29c7f54,
  abstract     = {Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases.},
  author       = {Lundwall, Åke and Dackowski, W. and Cohen, E. and Shaffer, M. and Mahr, A. and Dahlback, B. and Stenflo, J. and Wydro, R.},
  keyword      = {Protein S,Humans,Glycoproteins/analysis/*genetics,Epidermal Growth Factor/analysis,DNA/*analysis/isolation & purification,Amino Acid Sequence,Base Sequence,RNA,Messenger/analysis,Research Support,Non-U.S. Gov't},
  language     = {eng},
  number       = {18},
  pages        = {20--6716},
  series       = {Proc Natl Acad Sci U S A},
  title        = {Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation},
  volume       = {83},
  year         = {1986},
}