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Production of recombinant PSA and HK2 and analysis of their immunologic cross-reactivity

Lövgren, Janita LU ; Piironen, T.; Overmo, C.; Dowell, B.; Karp, M.; Pettersson, K.; Lilja, Hans LU and Lundwall, Åke LU (1995) In Biochem Biophys Res Commun 213(3). p.95-888
Abstract
Measurements of prostate-specific antigen (PSA) in serum are widely used to monitor patients with prostate cancer, but the attenuation of the assay response by PSA complexed to protease inhibitors has been shown to affect the results in certain assay designs. Moreover, the human glandular kallikrein-2 (hK2), a kallikrein-like serine protease that is 80% similar to PSA, might interfere with the specific detection of PSA by immunological cross-reactivity. We have expressed hK2 and PSA in eucaryotic cells using the Semliki Forest Virus expression system and studied the reactivity of 18 monoclonal anti-PSA IgGs. Five of them cross-reacted with identical affinities to recombinant hK2 whereas 13 recognized PSA alone. The antibodies that... (More)
Measurements of prostate-specific antigen (PSA) in serum are widely used to monitor patients with prostate cancer, but the attenuation of the assay response by PSA complexed to protease inhibitors has been shown to affect the results in certain assay designs. Moreover, the human glandular kallikrein-2 (hK2), a kallikrein-like serine protease that is 80% similar to PSA, might interfere with the specific detection of PSA by immunological cross-reactivity. We have expressed hK2 and PSA in eucaryotic cells using the Semliki Forest Virus expression system and studied the reactivity of 18 monoclonal anti-PSA IgGs. Five of them cross-reacted with identical affinities to recombinant hK2 whereas 13 recognized PSA alone. The antibodies that recognized both PSA and hK2 bind to a region of the protein that is exposed when PSA is complexed to alpha-1-antichymotrypsin. (Less)
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keywords
Kallikreins/*genetics/immunology, Humans, Cross Reactions, Molecular, Cloning, Monoclonal/immunology, Antibodies, Antigen-Antibody Reactions, Prostate-Specific Antigen/*genetics/immunology, Recombinant Proteins/genetics/immunology, Research Support, Non-U.S. Gov't, Semliki forest virus/genetics, Tissue Kallikreins
in
Biochem Biophys Res Commun
volume
213
issue
3
pages
95 - 888
publisher
Elsevier
language
English
LU publication?
yes
id
778c3948-8432-4910-b4a9-86c404cb8104 (old id 3965223)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7544581
date added to LUP
2013-08-11 18:52:52
date last changed
2016-10-05 14:43:15
@misc{778c3948-8432-4910-b4a9-86c404cb8104,
  abstract     = {Measurements of prostate-specific antigen (PSA) in serum are widely used to monitor patients with prostate cancer, but the attenuation of the assay response by PSA complexed to protease inhibitors has been shown to affect the results in certain assay designs. Moreover, the human glandular kallikrein-2 (hK2), a kallikrein-like serine protease that is 80% similar to PSA, might interfere with the specific detection of PSA by immunological cross-reactivity. We have expressed hK2 and PSA in eucaryotic cells using the Semliki Forest Virus expression system and studied the reactivity of 18 monoclonal anti-PSA IgGs. Five of them cross-reacted with identical affinities to recombinant hK2 whereas 13 recognized PSA alone. The antibodies that recognized both PSA and hK2 bind to a region of the protein that is exposed when PSA is complexed to alpha-1-antichymotrypsin.},
  author       = {Lövgren, Janita and Piironen, T. and Overmo, C. and Dowell, B. and Karp, M. and Pettersson, K. and Lilja, Hans and Lundwall, Åke},
  keyword      = {Kallikreins/*genetics/immunology,Humans,Cross Reactions,Molecular,Cloning,Monoclonal/immunology,Antibodies,Antigen-Antibody Reactions,Prostate-Specific Antigen/*genetics/immunology,Recombinant Proteins/genetics/immunology,Research Support,Non-U.S. Gov't,Semliki forest virus/genetics,Tissue Kallikreins},
  language     = {eng},
  number       = {3},
  pages        = {95--888},
  publisher    = {ARRAY(0x77f39a0)},
  series       = {Biochem Biophys Res Commun},
  title        = {Production of recombinant PSA and HK2 and analysis of their immunologic cross-reactivity},
  volume       = {213},
  year         = {1995},
}