Production of recombinant PSA and HK2 and analysis of their immunologic cross-reactivity
(1995) In Biochem Biophys Res Commun 213(3). p.888-895- Abstract
Measurements of prostate-specific antigen (PSA) in serum are widely used to monitor patients with prostate cancer, but the attenuation of the assay response by PSA complexed to protease inhibitors has been shown to affect the results in certain assay designs. Moreover, the human glandular kallikrein-2 (hK2), a kallikrein-like serine protease that is 80% similar to PSA, might interfere with the specific detection of PSA by immunological cross-reactivity. We have expressed hK2 and PSA in eucaryotic cells using the Semliki Forest Virus expression system and studied the reactivity of 18 monoclonal anti-PSA IgGs. Five of them cross-reacted with identical affinities to recombinant hK2 whereas 13 recognized PSA alone. The antibodies that... (More)
Measurements of prostate-specific antigen (PSA) in serum are widely used to monitor patients with prostate cancer, but the attenuation of the assay response by PSA complexed to protease inhibitors has been shown to affect the results in certain assay designs. Moreover, the human glandular kallikrein-2 (hK2), a kallikrein-like serine protease that is 80% similar to PSA, might interfere with the specific detection of PSA by immunological cross-reactivity. We have expressed hK2 and PSA in eucaryotic cells using the Semliki Forest Virus expression system and studied the reactivity of 18 monoclonal anti-PSA IgGs. Five of them cross-reacted with identical affinities to recombinant hK2 whereas 13 recognized PSA alone. The antibodies that recognized both PSA and hK2 bind to a region of the protein that is exposed when PSA is complexed to alpha-1-antichymotrypsin.
(Less)
- author
- Lövgren, Janita LU ; Piironen, T. ; Overmo, C. ; Dowell, B. ; Karp, M. ; Pettersson, K. ; Lilja, Hans LU and Lundwall, Åke LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Kallikreins/*genetics/immunology, Humans, Cross Reactions, Molecular, Cloning, Monoclonal/immunology, Antibodies, Antigen-Antibody Reactions, Prostate-Specific Antigen/*genetics/immunology, Recombinant Proteins/genetics/immunology, Research Support, Non-U.S. Gov't, Semliki forest virus/genetics, Tissue Kallikreins
- in
- Biochem Biophys Res Commun
- volume
- 213
- issue
- 3
- pages
- 888 - 895
- publisher
- Elsevier
- external identifiers
-
- scopus:0029086658
- pmid:7544581
- DOI
- 10.1006/bbrc.1995.2212
- language
- English
- LU publication?
- yes
- id
- 778c3948-8432-4910-b4a9-86c404cb8104 (old id 3965223)
- date added to LUP
- 2016-04-04 14:32:55
- date last changed
- 2023-02-10 15:47:56
@article{778c3948-8432-4910-b4a9-86c404cb8104, abstract = {{<p>Measurements of prostate-specific antigen (PSA) in serum are widely used to monitor patients with prostate cancer, but the attenuation of the assay response by PSA complexed to protease inhibitors has been shown to affect the results in certain assay designs. Moreover, the human glandular kallikrein-2 (hK2), a kallikrein-like serine protease that is 80% similar to PSA, might interfere with the specific detection of PSA by immunological cross-reactivity. We have expressed hK2 and PSA in eucaryotic cells using the Semliki Forest Virus expression system and studied the reactivity of 18 monoclonal anti-PSA IgGs. Five of them cross-reacted with identical affinities to recombinant hK2 whereas 13 recognized PSA alone. The antibodies that recognized both PSA and hK2 bind to a region of the protein that is exposed when PSA is complexed to alpha-1-antichymotrypsin.</p>}}, author = {{Lövgren, Janita and Piironen, T. and Overmo, C. and Dowell, B. and Karp, M. and Pettersson, K. and Lilja, Hans and Lundwall, Åke}}, keywords = {{Kallikreins/*genetics/immunology; Humans; Cross Reactions; Molecular; Cloning; Monoclonal/immunology; Antibodies; Antigen-Antibody Reactions; Prostate-Specific Antigen/*genetics/immunology; Recombinant Proteins/genetics/immunology; Research Support; Non-U.S. Gov't; Semliki forest virus/genetics; Tissue Kallikreins}}, language = {{eng}}, number = {{3}}, pages = {{888--895}}, publisher = {{Elsevier}}, series = {{Biochem Biophys Res Commun}}, title = {{Production of recombinant PSA and HK2 and analysis of their immunologic cross-reactivity}}, url = {{http://dx.doi.org/10.1006/bbrc.1995.2212}}, doi = {{10.1006/bbrc.1995.2212}}, volume = {{213}}, year = {{1995}}, }