Effects of Polylysine and Polyglutamate on Inflammation and the Normal Process of Peritoneal Healing After Surgery
(2012) In Journal of Tissue Science & Engineering 3(2). p.1-7- Abstract
- Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (αPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.
Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental... (More) - Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (αPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.
Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental groups received PL/PG on the surgery site. tPA, PAI-1, IL-6 and active TGFb1 were analyzed at given time points postoperatively in peritoneal lavage. Adhesions were evaluated after seven days. Significant differences were considered to be p<0.05.
Results: At a few individual time points small differences were seen between the groups (control and experiment) comparing levels of tPA, PAI-1, IL-6 and active TGFb1. When comparing levels of substances from all time points no statistical differences were seen between the groups as a total. Adhesions were significantly decreased on day 7, p=0.002.
Conclusion: Despite significant reduction in adhesions PL/PG administered intraperitoneally as an anti-adhesion agent locally on surgically traumatized area does not seem to affect the normal process of peritoneal healing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4333688
- author
- Åkerberg, Daniel LU ; Isaksson, Karolin LU ; Bauden, Monika LU ; Andersson, Roland LU and Tingstedt, Bobby LU
- organization
- publishing date
- 2012
- type
- Contribution to specialist publication or newspaper
- publication status
- published
- subject
- keywords
- Abdominal adhesions, Prevention, Polypeptides, Tissue plasminogen activator, Plasminogen activator inhibitor-1
- categories
- Popular Science
- in
- Journal of Tissue Science & Engineering
- volume
- 3
- issue
- 2
- pages
- 1 - 7
- ISSN
- 2157-7552
- DOI
- 10.4172/2157-7552.1000117
- language
- English
- LU publication?
- yes
- id
- c8b0cfd3-aa3b-44a5-a323-735a92477c00 (old id 4333688)
- date added to LUP
- 2016-04-04 09:40:24
- date last changed
- 2018-11-21 20:54:46
@misc{c8b0cfd3-aa3b-44a5-a323-735a92477c00, abstract = {{Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (αPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.<br/><br> Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental groups received PL/PG on the surgery site. tPA, PAI-1, IL-6 and active TGFb1 were analyzed at given time points postoperatively in peritoneal lavage. Adhesions were evaluated after seven days. Significant differences were considered to be p<0.05.<br/><br> Results: At a few individual time points small differences were seen between the groups (control and experiment) comparing levels of tPA, PAI-1, IL-6 and active TGFb1. When comparing levels of substances from all time points no statistical differences were seen between the groups as a total. Adhesions were significantly decreased on day 7, p=0.002.<br/><br> Conclusion: Despite significant reduction in adhesions PL/PG administered intraperitoneally as an anti-adhesion agent locally on surgically traumatized area does not seem to affect the normal process of peritoneal healing.}}, author = {{Åkerberg, Daniel and Isaksson, Karolin and Bauden, Monika and Andersson, Roland and Tingstedt, Bobby}}, issn = {{2157-7552}}, keywords = {{Abdominal adhesions; Prevention; Polypeptides; Tissue plasminogen activator; Plasminogen activator inhibitor-1}}, language = {{eng}}, number = {{2}}, pages = {{1--7}}, series = {{Journal of Tissue Science & Engineering}}, title = {{Effects of Polylysine and Polyglutamate on Inflammation and the Normal Process of Peritoneal Healing After Surgery}}, url = {{http://dx.doi.org/10.4172/2157-7552.1000117}}, doi = {{10.4172/2157-7552.1000117}}, volume = {{3}}, year = {{2012}}, }