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Effects of Polylysine and Polyglutamate on Inflammation and the Normal Process of Peritoneal Healing After Surgery

Åkerberg, Daniel LU ; Isaksson, Karolin LU ; Bauden, Monika LU ; Andersson, Roland LU and Tingstedt, Bobby LU (2012) In Journal of Tissue Science & Engineering 3(2). p.1-7
Abstract
Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (αPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.

Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental... (More)
Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (αPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.

Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental groups received PL/PG on the surgery site. tPA, PAI-1, IL-6 and active TGFb1 were analyzed at given time points postoperatively in peritoneal lavage. Adhesions were evaluated after seven days. Significant differences were considered to be p<0.05.

Results: At a few individual time points small differences were seen between the groups (control and experiment) comparing levels of tPA, PAI-1, IL-6 and active TGFb1. When comparing levels of substances from all time points no statistical differences were seen between the groups as a total. Adhesions were significantly decreased on day 7, p=0.002.

Conclusion: Despite significant reduction in adhesions PL/PG administered intraperitoneally as an anti-adhesion agent locally on surgically traumatized area does not seem to affect the normal process of peritoneal healing. (Less)
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author
organization
publishing date
type
Contribution to specialist publication or newspaper
publication status
published
subject
keywords
Abdominal adhesions, Prevention, Polypeptides, Tissue plasminogen activator, Plasminogen activator inhibitor-1
categories
Popular Science
in
Journal of Tissue Science & Engineering
volume
3
issue
2
pages
1 - 7
ISSN
2157-7552
DOI
10.4172/2157-7552.1000117
language
English
LU publication?
yes
id
c8b0cfd3-aa3b-44a5-a323-735a92477c00 (old id 4333688)
date added to LUP
2016-01-28 12:15:59
date last changed
2016-04-16 07:03:05
@misc{c8b0cfd3-aa3b-44a5-a323-735a92477c00,
  abstract     = {Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (αPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.<br/><br>
Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental groups received PL/PG on the surgery site. tPA, PAI-1, IL-6 and active TGFb1 were analyzed at given time points postoperatively in peritoneal lavage. Adhesions were evaluated after seven days. Significant differences were considered to be p&lt;0.05.<br/><br>
Results: At a few individual time points small differences were seen between the groups (control and experiment) comparing levels of tPA, PAI-1, IL-6 and active TGFb1. When comparing levels of substances from all time points no statistical differences were seen between the groups as a total. Adhesions were significantly decreased on day 7, p=0.002.<br/><br>
Conclusion: Despite significant reduction in adhesions PL/PG administered intraperitoneally as an anti-adhesion agent locally on surgically traumatized area does not seem to affect the normal process of peritoneal healing.},
  author       = {Åkerberg, Daniel and Isaksson, Karolin and Bauden, Monika and Andersson, Roland and Tingstedt, Bobby},
  issn         = {2157-7552},
  keyword      = {Abdominal adhesions,Prevention,Polypeptides,Tissue plasminogen activator,Plasminogen activator inhibitor-1},
  language     = {eng},
  number       = {2},
  pages        = {1--7},
  series       = {Journal of Tissue Science & Engineering},
  title        = {Effects of Polylysine and Polyglutamate on Inflammation and the Normal Process of Peritoneal Healing After Surgery},
  url          = {http://dx.doi.org/10.4172/2157-7552.1000117},
  volume       = {3},
  year         = {2012},
}