Studies of Electrocatalytic Reactions of NADH at Mediator Modified Electrodes and of H2O2 at Peroxidase Modified Electrodes

Abstract

This thesis addresses: (1) studies of direct and mediated electron transfer and kinetics of peroxidases on electrodes and the development of amperometric peroxidase-based biosenors, (2) electrochemical and kinetic studies of NADH oxidation at mediator modified electrodes.



The direct and mediated electron transfer of horseradish and tobacco peroxidase were investigated on graphite electrodes by using wall-jet electrode methodology.



Biosensors based on novel plant peroxidases for determination of phenolic compounds were developed.



The electrochemical properties of different redox mediators immobilised on titanium phosphate, zirconium phosphate or silica gel were studied by means of cyclic voltammetry. The mediator properties for electrocatalytic oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) at the chemically modified electrodes are presented. For the kinetic investigations the rotating disk electrode methodology was used.



The interaction between some of the mediators and titanium phosphate was investigated by means of attenuated reflectance infrared spectroscopy and the possible leakage of the mediators from the electrode was investigated by using fast scan cyclic voltammetry and scanning electrochemical microscopy.

Abstract (Swedish)

Popular Abstract in Swedish

Många biokemiska reaktioner har höga aktiveringsenergier och utan de katalyserande enzymerna skulle de flesta ämnes- omsättningsprocesser fortskrida alldeles för långsamt för att uppehålla liv. Enzymer kallas ofta för biokatalysatorer eftersom de verkar katalytiskt, dvs påskyndar kemiska reaktioner utan att själva förändras. De finns i en enorm variation eftersom vart och ett oftast bara är inblandat i en reaktion. Enzymerna behöver bara finnas i ytterst små mängder för att påverka hastigheten för en reaktion.



I denna avhandling elektrokemiska biosensorer använts och i avhandlingen ingår studier av direkt och medierad elektronöverföring mellan immobiliserade enzymer (peroxidaser) och elektrodensyta, och studier om NADH oxidering med hjälp av mediator modifierade elektroder. Dessutom har biosensorer baserade på peroxidaser utvecklats för detektion av fenoliska ämnen i miljöprover.

• author: Munteanu, Florentina
• supervisor: [unknown] [unknown]
• opponent: Prof. McNeil, Calum, University of Newcastle Upon Tyne, UK
• organization: Centre for Analysis and Synthesis
• publishing date: 2002
• type: Thesis
• publication status: published
• subject: Analytical Chemistry
• keywords: Electrochemistry, Elektrokemi, kinetic studies, silica gel, titanium phoshate, zirconium phosphate, NADH, redox mediator, phenolic compounds, hydrogen peroxide, electron transfer, graphite and carbon paste electrodes, peroxidase, biosensor
• pages: 208 pages
• defense location: Kemicentrum, Sölvegatan 39, Lund, hörsal B
• defense date: 2002-09-06 10:15:00
• ISBN: 91-7422-005-5
• language: English
• LU publication?: yes
• additional info: Article: I. Bioelectrochemical Monitoring of Phenols and Aromatic Amines in Flow Injection Using Novel Plant PeroxidasesF.-D. Munteanu, A. Lindgren, J. Émneus, L. Gorton, T. Ruzgas, E. Csöregi, A. Ciucu, R. B. van Huystee, I. G. Gazaryan, and L. M. LagriminiAnalytical Chemistry, 1998, 70, 2596 Article: II. Comparison of Rotating Disk and Wall-Jet Electrode Systems for Studying the Kinetics Of Direct and Mediated Electron Transfer for Horseradish Peroxidase on a Graphite ElectrodeA. Lindgren, F.-D. Munteanu, I. G. Gazaryan, T. Ruzgas, L. Gorton,Journal of Elecroanalytical Chemistry, 1998, 458, 113 Article: III. Direct and Mediated Electron Transfer Catalysed by Anionic Tobacco Peroxidase: Effect of Calcium IonsF.-D Munteanu, L. Gorton, A. Lindgren, T. Ruzgas, J. Émneus, E. Csöregi, I. G. Gazaryan, I.V. Ouporov, E. A. Mareeva and L. M. LagriminiApplied Biochemistry and Biotechnology, 2000, 88, 321 Article: IV. Electrochemical Investigation of Some Aromatic Redox Mediators Immobilised on Titanium PhosphateL. Kubota, F.-D. Munteanu, A. Roddick-Lanzilotta, A. J. McQuillan, and L. GortonQuímica Analitica, 2000, 19 (Suppl 1) 15 Article: V. Effect of pH on the Catalytic Electrooxidation of NADH Using Different 2-Electron Mediators Immobilised on Zirconium PhosphateF.-D. Munteanu, L. Kubota, and L. Gorton,Journal of Electroanalytical Chemistry, 2001, 509 (1), 2; Article: VI. NADH Electroxidation Using Nitro-Fluorenone Derivatives Immobilised on Zirconium PhosphateF.-D. Munteanu, N. Mano, A.Kuhn, and L. GortonAnalytical Chemistry, 2002, submitted Article: VII. NADH Electroxidation Using Dibenzo-Dithia-Diazapentacene Modified Carbon Paste ElectrodesF.-D. Munteanu, D. Dicu, I.C. Popescu, L. GortonElectroanalysis, 2002, submitted Article: VIII. Electrochemical and Catalytic Investigation Of Carbon Paste Modified with Toluidine Blue O Covalently Immobilised on Silica GelF.-D. Munteanu, L. Gorton, Y. OkamotoAnalitica Chimica Acta, 2002, submitted Article: IX. Fast Scan Cyclic Voltammetry and Scanning Electrochemical Microscopy for Studies on the Acidic pH Effect on 2-electron Mediators Immobilised on Zirconium PhosphateF.-D. Munteanu, M. Mosbach, A. Schulte, W. Schuhmann, and L. GortonElectroanalysis, 2002, accepted; Article: Appendix I. Direct Electron Transfer Between Heme-Containing Enzymes and Electrodes as Basis for Third Generation BiosensorsL. Gorton, A. Lindgren, T. Larsson, F.-D. Munteanu, T. Ruzgas and I.G. Gazaryan,Analitica Chimica Acta, 1999, 400, 91; The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
• id: 46acff16-8811-45f6-b9a1-ea14f620d98b (old id 464787)
• date added to LUP: 2016-04-04 09:54:50
• date last changed: 2018-11-21 20:55:39