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Genotyping of Staphylococcus aureus in bovine mastitis and correlation to phenotypic characteristics

Artursson, Karin; Söderlund, Robert; Liu, Lihong; Monecke, Stefan and Schelin, Jenny LU (2016) In Veterinary Microbiology 193. p.156-161
Abstract

Reducing the prevalence of mastitis caused by Staphylococcus aureus (S. aureus) is essential to improve animal health and reduce economic losses for farmers. The clinical outcome of acute mastitis and risk of progression to persistent mastitis can, at least to some extent, be related to genetic variants of the strain causing the infection. In the present study we have used microarrays to investigate the presence of virulence genes in S. aureus isolates from dairy cows with acute clinical mastitis (n = 70) and correlated the findings to other genotypic and phenotypic characteristics. Among the most commonly found virulence factors were genes encoding several hemolysin types, leukocidins D and lukM/lukF-P83, clumping factors A and B,... (More)

Reducing the prevalence of mastitis caused by Staphylococcus aureus (S. aureus) is essential to improve animal health and reduce economic losses for farmers. The clinical outcome of acute mastitis and risk of progression to persistent mastitis can, at least to some extent, be related to genetic variants of the strain causing the infection. In the present study we have used microarrays to investigate the presence of virulence genes in S. aureus isolates from dairy cows with acute clinical mastitis (n = 70) and correlated the findings to other genotypic and phenotypic characteristics. Among the most commonly found virulence factors were genes encoding several hemolysin types, leukocidins D and lukM/lukF-P83, clumping factors A and B, fibrinogen binding protein and fibronectin-binding protein A. Some virulence factors e.g. fibronectin-binding protein B and Staphylococcus aureus surface protein G were less common. Genes coding for several staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1) were commonly found, especially in one major pulsotype. No beta-lactamase genes were found in any common pulsotype, while present in some rare pulsotypes, indicated to be of human origin. Production of TSST-1, enterotoxins, hemolysins and beta-lactamase could all be positively correlated to presence of the corresponding genes. This study reveals a number of genotypic differences and similarities among common and rare pulsotypes of S. aureus from cases of mastitis in Sweden. The results could help the design of diagnostic tools to guide on-farm interventions according to the expected impact on udder health from a specific S. aureus genotype.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Genotyping, Mastitis, Microarray, Staphylococcus aureus, Virulence gene
in
Veterinary Microbiology
volume
193
pages
6 pages
publisher
Elsevier
external identifiers
  • Scopus:84983432490
ISSN
0378-1135
DOI
10.1016/j.vetmic.2016.08.012
language
English
LU publication?
yes
id
47d432e7-19f6-490a-b4b6-9455c5b28318
date added to LUP
2016-10-04 07:31:43
date last changed
2016-10-05 03:00:06
@misc{47d432e7-19f6-490a-b4b6-9455c5b28318,
  abstract     = {<p>Reducing the prevalence of mastitis caused by Staphylococcus aureus (S. aureus) is essential to improve animal health and reduce economic losses for farmers. The clinical outcome of acute mastitis and risk of progression to persistent mastitis can, at least to some extent, be related to genetic variants of the strain causing the infection. In the present study we have used microarrays to investigate the presence of virulence genes in S. aureus isolates from dairy cows with acute clinical mastitis (n = 70) and correlated the findings to other genotypic and phenotypic characteristics. Among the most commonly found virulence factors were genes encoding several hemolysin types, leukocidins D and lukM/lukF-P83, clumping factors A and B, fibrinogen binding protein and fibronectin-binding protein A. Some virulence factors e.g. fibronectin-binding protein B and Staphylococcus aureus surface protein G were less common. Genes coding for several staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1) were commonly found, especially in one major pulsotype. No beta-lactamase genes were found in any common pulsotype, while present in some rare pulsotypes, indicated to be of human origin. Production of TSST-1, enterotoxins, hemolysins and beta-lactamase could all be positively correlated to presence of the corresponding genes. This study reveals a number of genotypic differences and similarities among common and rare pulsotypes of S. aureus from cases of mastitis in Sweden. The results could help the design of diagnostic tools to guide on-farm interventions according to the expected impact on udder health from a specific S. aureus genotype.</p>},
  author       = {Artursson, Karin and Söderlund, Robert and Liu, Lihong and Monecke, Stefan and Schelin, Jenny},
  issn         = {0378-1135},
  keyword      = {Genotyping,Mastitis,Microarray,Staphylococcus aureus,Virulence gene},
  language     = {eng},
  month        = {09},
  pages        = {156--161},
  publisher    = {ARRAY(0x975ca68)},
  series       = {Veterinary Microbiology},
  title        = {Genotyping of Staphylococcus aureus in bovine mastitis and correlation to phenotypic characteristics},
  url          = {http://dx.doi.org/10.1016/j.vetmic.2016.08.012},
  volume       = {193},
  year         = {2016},
}