Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondria
(1990) In FEBS Letters 259(2). p.311-314- Abstract
In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in... (More)
In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity.
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- author
- Rasmusson, Allan G. LU ; Møller, Ian M. and Palmer, John M. LU
- organization
- publishing date
- 1990-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (Arum maculatum, Plant, Mitochondria), Alternative pathway, Inside-out submitochondrial particle, Mitoplast, Trypsin
- in
- FEBS Letters
- volume
- 259
- issue
- 2
- pages
- 4 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0025034768
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(90)80034-G
- language
- English
- LU publication?
- yes
- id
- 48323140-1eaf-4921-ba01-353de3ccf445
- date added to LUP
- 2016-05-31 21:43:01
- date last changed
- 2024-01-04 07:47:49
@article{48323140-1eaf-4921-ba01-353de3ccf445, abstract = {{<p>In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity.</p>}}, author = {{Rasmusson, Allan G. and Møller, Ian M. and Palmer, John M.}}, issn = {{0014-5793}}, keywords = {{(Arum maculatum, Plant, Mitochondria); Alternative pathway; Inside-out submitochondrial particle; Mitoplast; Trypsin}}, language = {{eng}}, month = {{01}}, number = {{2}}, pages = {{311--314}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondria}}, url = {{http://dx.doi.org/10.1016/0014-5793(90)80034-G}}, doi = {{10.1016/0014-5793(90)80034-G}}, volume = {{259}}, year = {{1990}}, }