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Failure of bone induction by bone matrix in adult monkeys

Aspenberg, P. LU ; Lohmander, Stefan LU and Thorngren, K. G. LU (1988) In Journal of Bone and Joint Surgery 70(4). p.625-627
Abstract

Extraskeletal bone formation can be induced in rodents by implantation of demineralised bone matrix and such implantation has been used to treat bone defects in man, but it is uncertain if induction or merely conduction occurs. We studied bone induction in primates by excising segments of the fibulae of adult squirrel monkeys, defatting and demineralising them before reimplanting them into the quadriceps of the same animal. As a control experiment, rat matrix was prepared in exactly the same way and implanted in rats. After six weeks the implants were harvested and either ashed and analysed for calcium content or prepared for histology. In the rats, the calcium content indicated that about 20% of the original matrix had been replaced by... (More)

Extraskeletal bone formation can be induced in rodents by implantation of demineralised bone matrix and such implantation has been used to treat bone defects in man, but it is uncertain if induction or merely conduction occurs. We studied bone induction in primates by excising segments of the fibulae of adult squirrel monkeys, defatting and demineralising them before reimplanting them into the quadriceps of the same animal. As a control experiment, rat matrix was prepared in exactly the same way and implanted in rats. After six weeks the implants were harvested and either ashed and analysed for calcium content or prepared for histology. In the rats, the calcium content indicated that about 20% of the original matrix had been replaced by new bone. In the monkeys the calcium content was about the same as that in normal body fluid and no bone was seen in histological sections. This result casts doubt on the use of demineralised human bone matrix as a bone inductor, although it may function by other mechanisms.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
animals, bone formation , bone matrix
in
Journal of Bone and Joint Surgery
volume
70
issue
4
pages
3 pages
publisher
British Editorial Society of Bone and Joint Surgery
external identifiers
  • Scopus:0023809044
ISSN
0301-620X
language
English
LU publication?
yes
id
5050803f-664a-454a-bc95-0ef28e04ce18
date added to LUP
2016-05-04 13:06:55
date last changed
2016-05-25 12:55:33
@misc{5050803f-664a-454a-bc95-0ef28e04ce18,
  abstract     = {<p>Extraskeletal bone formation can be induced in rodents by implantation of demineralised bone matrix and such implantation has been used to treat bone defects in man, but it is uncertain if induction or merely conduction occurs. We studied bone induction in primates by excising segments of the fibulae of adult squirrel monkeys, defatting and demineralising them before reimplanting them into the quadriceps of the same animal. As a control experiment, rat matrix was prepared in exactly the same way and implanted in rats. After six weeks the implants were harvested and either ashed and analysed for calcium content or prepared for histology. In the rats, the calcium content indicated that about 20% of the original matrix had been replaced by new bone. In the monkeys the calcium content was about the same as that in normal body fluid and no bone was seen in histological sections. This result casts doubt on the use of demineralised human bone matrix as a bone inductor, although it may function by other mechanisms.</p>},
  author       = {Aspenberg, P. and Lohmander, Stefan and Thorngren, K. G.},
  issn         = {0301-620X},
  keyword      = {animals,bone formation ,bone matrix},
  language     = {eng},
  number       = {4},
  pages        = {625--627},
  publisher    = {ARRAY(0xa7c09a0)},
  series       = {Journal of Bone and Joint Surgery},
  title        = {Failure of bone induction by bone matrix in adult monkeys},
  volume       = {70},
  year         = {1988},
}