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Effect of carbon dioxide and temperature on H2 producing cultures of the extreme thermophile, Caldicellulosiruptor saccharolyticus

van Niel, Ed LU ; Martens, A.A. and Claassen, P.A.M. (2004) 15th World Hydrogen Energy Conference In Proceedings of the 15th World Hydrogen Energy Conference
Abstract
Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower the maximum allowable acetate concentration in culture, and therefore the productivity of the culture at neutral pH. Although the growth rate was not affected by CO2, lower biomass and hydrogen yields were obtained. This could be due to an increased... (More)
Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower the maximum allowable acetate concentration in culture, and therefore the productivity of the culture at neutral pH. Although the growth rate was not affected by CO2, lower biomass and hydrogen yields were obtained. This could be due to an increased lactate production. In a thermodynamic study an estimation of the activation enthalpies was made for both hydrogen production and its thermal inactivation, being 45.9 kJ/mol and 112.3 kJ/mol, respectively. Hydrogen production showed a wider temperature range (estimated 40 - 90° C) than growth (49.6 – 85.7° C). (Less)
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Chapter in Book/Report/Conference proceeding
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published
subject
keywords
Caldicellulosiruptor saccharolyticus, osmolality, activation enthalpy, carbon dioxide, extreme thermophile
in
Proceedings of the 15th World Hydrogen Energy Conference
pages
10 pages
conference name
15th World Hydrogen Energy Conference
language
English
LU publication?
yes
id
f04a781d-f6a3-4244-b5fe-eeb0d155bedd (old id 530649)
date added to LUP
2007-09-25 13:31:00
date last changed
2016-04-16 11:44:51
@misc{f04a781d-f6a3-4244-b5fe-eeb0d155bedd,
  abstract     = {Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower the maximum allowable acetate concentration in culture, and therefore the productivity of the culture at neutral pH. Although the growth rate was not affected by CO2, lower biomass and hydrogen yields were obtained. This could be due to an increased lactate production. In a thermodynamic study an estimation of the activation enthalpies was made for both hydrogen production and its thermal inactivation, being 45.9 kJ/mol and 112.3 kJ/mol, respectively. Hydrogen production showed a wider temperature range (estimated 40 - 90° C) than growth (49.6 – 85.7° C).},
  author       = {van Niel, Ed and Martens, A.A. and Claassen, P.A.M.},
  keyword      = {Caldicellulosiruptor saccharolyticus,osmolality,activation enthalpy,carbon dioxide,extreme thermophile},
  language     = {eng},
  pages        = {10},
  series       = {Proceedings of the 15th World Hydrogen Energy Conference},
  title        = {Effect of carbon dioxide and temperature on H2 producing cultures of the extreme thermophile, Caldicellulosiruptor saccharolyticus},
  year         = {2004},
}