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Studies on Peripheral Nerve Regeneration: Microdispensing, Neurite Guidance and Galectins

Gustavsson, Per LU (2007)
Abstract (Swedish)
Popular Abstract in Swedish

Denna avhandling beskriver utvecklingen av en teknik baserad på mikrodispensering, för att skapa proteinmönster som kan användas för styrning av axoner. Samma teknik vidareutvecklades för att kunna positionera dissocierade celler, bl.a. nervceller, på cellodlingsytor. Slutligen undersöktes vilken roll galektin-3 och 8 har under nervregeneration. Avhandlingen består av fyra artiklar (I-IV).



Mikrodispensering är en metod som liknar bläckstråleskrivartekniken och kan användas för att placera små volymer vätska på substrat med hög precision. Metoden visade sig vara ett snabbt och noggrant sätt att skapa proteinmönster på cellodlingssubstrat. Regenererande axoner från... (More)
Popular Abstract in Swedish

Denna avhandling beskriver utvecklingen av en teknik baserad på mikrodispensering, för att skapa proteinmönster som kan användas för styrning av axoner. Samma teknik vidareutvecklades för att kunna positionera dissocierade celler, bl.a. nervceller, på cellodlingsytor. Slutligen undersöktes vilken roll galektin-3 och 8 har under nervregeneration. Avhandlingen består av fyra artiklar (I-IV).



Mikrodispensering är en metod som liknar bläckstråleskrivartekniken och kan användas för att placera små volymer vätska på substrat med hög precision. Metoden visade sig vara ett snabbt och noggrant sätt att skapa proteinmönster på cellodlingssubstrat. Regenererande axoner från dorsalrotsganglier undvek mönster gjorda av bovint serumalbumin och föredrog istället att växa på mikrodispenserat laminin (I). Mikrodispensering kunde även användas för att positionera celler direkt på odlingssubstrat utan att cellerna tog skada. Tekniken kunde användas för att konstruera tvådimensionella miniatyrorgan, t.ex. ett tvådimensionellt dorsalrotsganglion (II). Galektin-3 och 8 tillhör en familj av lektiner (kolhydratsbindande proteiner) som uttrycks i djurceller. Studier visade att dessa galektiner undertryckte axonal utväxt i extracellulärmatrixsubstitutet ECM-gel. När galektin-3 och 8 användes för att göra mikrodispenserade proteinmönster hade de ingen effekt på axonal guidning (III). Slutligen visade det sig att galektin-3 inhiberade Schwanncellsproliferation i odlade bitar av ischiasnerver. Inhibitionen visade sig vara oberonde av lektinbindning (IV). (Less)
Abstract
The present thesis describes development of a microdispenser-based technique to create of protein patterns for neurite guidance. The same technique was also developed to allow direct positioning of dissociated cells, including neurons, on cell culture substrates. Finally the role of galectin-3 and 8 during nerve regeneration was investigated. The thesis consists of four papers (I-IV).



Microdispensing is a method similar to inkjet printing, which can be used for precise patterning of small volumes of liquids on substrates. The method proved to be a rapid and accurate instrument for the creation of protein patterns on cell culture substrates. Patterns made of bovine serum albumin were avoided by regenerating neurites from... (More)
The present thesis describes development of a microdispenser-based technique to create of protein patterns for neurite guidance. The same technique was also developed to allow direct positioning of dissociated cells, including neurons, on cell culture substrates. Finally the role of galectin-3 and 8 during nerve regeneration was investigated. The thesis consists of four papers (I-IV).



Microdispensing is a method similar to inkjet printing, which can be used for precise patterning of small volumes of liquids on substrates. The method proved to be a rapid and accurate instrument for the creation of protein patterns on cell culture substrates. Patterns made of bovine serum albumin were avoided by regenerating neurites from dorsal root ganglia, which instead preferred to grow on microdispensed laminin (I). Microdispensing could also be used to position cell directly on culture substrates without harming the cells. The technique was used for the construction of 2D miniature organs, i.e. a 2D dorsal root ganglion (II).



Galectin-3 and 8 (belonging to a family of animal lectins) were shown to inhibit neurite outgrowth in extracellular matrix substitute (ECM gel) but had no effects on neurite guidance when microdispensed on plastic surfaces (III). In addition, galectin-3 was shown to inhibit the proliferation of Schwann cells in cultured sciatic nerve segments, an effect independent of its lectin properties (IV). (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Dr. Hermansson, Ola, Department of Neuroscience, Karolinska Institutet, Stockholm
organization
publishing date
type
Thesis
publication status
published
subject
keywords
vävnadskultur, histokemi, cytokemi, Histologi, tissue culture, histochemistry, cytochemistry, Histology, Schwann cells, sciatic nerve, dorsal root ganglia, DRG, neurite guidance, galectins, inkjet, microdispensing, peripheral nervous system, nerve regeneration, Animal physiology, Djurfysiologi, Biotechnology, Bioteknik
pages
157 pages
publisher
Department of Cell and Organism Biology, Lund University
defense location
Animal Physiology Building Helgonavägen 3B Lund
defense date
2007-02-09 09:00
ISBN
10 91-85067-29-6
978-91-85067-29-9
language
English
LU publication?
yes
id
ac234d63-2d21-4e25-987a-18077a8eec55 (old id 547917)
date added to LUP
2007-09-04 15:49:54
date last changed
2016-09-19 08:45:10
@misc{ac234d63-2d21-4e25-987a-18077a8eec55,
  abstract     = {The present thesis describes development of a microdispenser-based technique to create of protein patterns for neurite guidance. The same technique was also developed to allow direct positioning of dissociated cells, including neurons, on cell culture substrates. Finally the role of galectin-3 and 8 during nerve regeneration was investigated. The thesis consists of four papers (I-IV).<br/><br>
<br/><br>
Microdispensing is a method similar to inkjet printing, which can be used for precise patterning of small volumes of liquids on substrates. The method proved to be a rapid and accurate instrument for the creation of protein patterns on cell culture substrates. Patterns made of bovine serum albumin were avoided by regenerating neurites from dorsal root ganglia, which instead preferred to grow on microdispensed laminin (I). Microdispensing could also be used to position cell directly on culture substrates without harming the cells. The technique was used for the construction of 2D miniature organs, i.e. a 2D dorsal root ganglion (II).<br/><br>
<br/><br>
Galectin-3 and 8 (belonging to a family of animal lectins) were shown to inhibit neurite outgrowth in extracellular matrix substitute (ECM gel) but had no effects on neurite guidance when microdispensed on plastic surfaces (III). In addition, galectin-3 was shown to inhibit the proliferation of Schwann cells in cultured sciatic nerve segments, an effect independent of its lectin properties (IV).},
  author       = {Gustavsson, Per},
  isbn         = {10 91-85067-29-6},
  keyword      = {vävnadskultur,histokemi,cytokemi,Histologi,tissue culture,histochemistry,cytochemistry,Histology,Schwann cells,sciatic nerve,dorsal root ganglia,DRG,neurite guidance,galectins,inkjet,microdispensing,peripheral nervous system,nerve regeneration,Animal physiology,Djurfysiologi,Biotechnology,Bioteknik},
  language     = {eng},
  pages        = {157},
  publisher    = {ARRAY(0x8357308)},
  title        = {Studies on Peripheral Nerve Regeneration: Microdispensing, Neurite Guidance and Galectins},
  year         = {2007},
}