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Purification of a rotenone-insensitive NAD(P)H dehydrogenase from the inner surface of the inner membrane of red beetroot mitochondria

Rasmusson, Allan G. LU ; Fredlund, Kenneth M. and Møller, Ian M. (1993) In Biochimica et Biophysica Acta - Bioenergetics 1141(1). p.107-110
Abstract

The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into a 26 kDa polypeptide. © 1993.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
(Plant), Enzyme purification, Inner membrane, Mitochondrion, NAD(P)H dehydrogenase, Protein purification, Respiratory chain, Rotenone insensitivity
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1141
issue
1
pages
4 pages
publisher
Elsevier
external identifiers
  • Scopus:0027471467
ISSN
0005-2728
DOI
10.1016/0005-2728(93)90196-M
language
English
LU publication?
yes
id
555221c2-6ab7-4d5d-8ec8-3487b2ff21ba
date added to LUP
2016-05-31 21:39:03
date last changed
2016-08-10 14:38:31
@misc{555221c2-6ab7-4d5d-8ec8-3487b2ff21ba,
  abstract     = {<p>The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into a 26 kDa polypeptide. © 1993.</p>},
  author       = {Rasmusson, Allan G. and Fredlund, Kenneth M. and Møller, Ian M.},
  issn         = {0005-2728},
  keyword      = {(Plant),Enzyme purification,Inner membrane,Mitochondrion,NAD(P)H dehydrogenase,Protein purification,Respiratory chain,Rotenone insensitivity},
  language     = {eng},
  month        = {02},
  number       = {1},
  pages        = {107--110},
  publisher    = {ARRAY(0xa5c3f08)},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Purification of a rotenone-insensitive NAD(P)H dehydrogenase from the inner surface of the inner membrane of red beetroot mitochondria},
  url          = {http://dx.doi.org/10.1016/0005-2728(93)90196-M},
  volume       = {1141},
  year         = {1993},
}