Naturally Occurring IgG Antibodies Provide Innate Protection against Vibrio cholerae Bacteremia by Recognition of the Outer Membrane Protein U
(2016) In Journal of Innate Immunity 8(3). p.269-283- Abstract
Cholera epidemics are caused by Vibrio cholerae serogroups O1 and O139, whereas strains collectively known as non-O1/non-O139 V. cholerae are found in cases of extraintestinal infections and bacteremia. The mechanisms and factors influencing the occurrence of bacteremia and survival of V. cholerae in normal human serum have remained unclear. We found that naturally occurring IgG recognizing V. cholerae outer membrane protein U (OmpU) mediates a serum-killing effect in a complement C1q-dependent manner. Moreover, outer membrane vesicles (OMVs) containing OmpU caused enhanced survival of highly serum-sensitive classical V. cholerae in a dose-dependent manner. OMVs from wild-type and ompU mutant V. cholerae thereby provided a novel means... (More)
Cholera epidemics are caused by Vibrio cholerae serogroups O1 and O139, whereas strains collectively known as non-O1/non-O139 V. cholerae are found in cases of extraintestinal infections and bacteremia. The mechanisms and factors influencing the occurrence of bacteremia and survival of V. cholerae in normal human serum have remained unclear. We found that naturally occurring IgG recognizing V. cholerae outer membrane protein U (OmpU) mediates a serum-killing effect in a complement C1q-dependent manner. Moreover, outer membrane vesicles (OMVs) containing OmpU caused enhanced survival of highly serum-sensitive classical V. cholerae in a dose-dependent manner. OMVs from wild-type and ompU mutant V. cholerae thereby provided a novel means to verify by extracellular transcomplementation the involvement of OmpU. Our data conclusively indicate that loss, or reduced expression, of OmpU imparts resistance to V. cholerae towards serum killing. We propose that the difference in OmpU protein levels is a plausible reason for differences in serum resistance and the ability to cause bacteremia observed among V. cholerae biotypes. Our findings provide a new perspective on how naturally occurring antibodies, perhaps induced by members of the microbiome, may play a role in the recognition of pathogens and the provocation of innate immune defense against bacteremia.
(Less)
- author
- Aung, Kyaw Min ; Sjöström, Annika E. ; von Pawel-Rammingen, Ulrich ; Riesbeck, Kristian LU ; Uhlin, Bernt Eric and Wai, Sun Nyunt
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- <italic>Vibrio cholerae</italic>, C1q#, Naturally occurring IgG antibodies, Outer membrane protein U, Outer membrane vesicles, Serum resistance
- in
- Journal of Innate Immunity
- volume
- 8
- issue
- 3
- pages
- 269 - 283
- publisher
- Karger
- external identifiers
-
- wos:000375803900006
- pmid:26934383
- scopus:84961223388
- ISSN
- 1662-811X
- DOI
- 10.1159/000443646
- language
- English
- LU publication?
- yes
- id
- 5e18c9e0-c20e-47ce-9ab9-908d02a09e2d
- date added to LUP
- 2016-07-08 11:05:24
- date last changed
- 2024-06-14 10:59:44
@article{5e18c9e0-c20e-47ce-9ab9-908d02a09e2d, abstract = {{<p>Cholera epidemics are caused by Vibrio cholerae serogroups O1 and O139, whereas strains collectively known as non-O1/non-O139 V. cholerae are found in cases of extraintestinal infections and bacteremia. The mechanisms and factors influencing the occurrence of bacteremia and survival of V. cholerae in normal human serum have remained unclear. We found that naturally occurring IgG recognizing V. cholerae outer membrane protein U (OmpU) mediates a serum-killing effect in a complement C1q-dependent manner. Moreover, outer membrane vesicles (OMVs) containing OmpU caused enhanced survival of highly serum-sensitive classical V. cholerae in a dose-dependent manner. OMVs from wild-type and ompU mutant V. cholerae thereby provided a novel means to verify by extracellular transcomplementation the involvement of OmpU. Our data conclusively indicate that loss, or reduced expression, of OmpU imparts resistance to V. cholerae towards serum killing. We propose that the difference in OmpU protein levels is a plausible reason for differences in serum resistance and the ability to cause bacteremia observed among V. cholerae biotypes. Our findings provide a new perspective on how naturally occurring antibodies, perhaps induced by members of the microbiome, may play a role in the recognition of pathogens and the provocation of innate immune defense against bacteremia.</p>}}, author = {{Aung, Kyaw Min and Sjöström, Annika E. and von Pawel-Rammingen, Ulrich and Riesbeck, Kristian and Uhlin, Bernt Eric and Wai, Sun Nyunt}}, issn = {{1662-811X}}, keywords = {{<italic>Vibrio cholerae</italic>; C1q#; Naturally occurring IgG antibodies; Outer membrane protein U; Outer membrane vesicles; Serum resistance}}, language = {{eng}}, number = {{3}}, pages = {{269--283}}, publisher = {{Karger}}, series = {{Journal of Innate Immunity}}, title = {{Naturally Occurring IgG Antibodies Provide Innate Protection against Vibrio cholerae Bacteremia by Recognition of the Outer Membrane Protein U}}, url = {{http://dx.doi.org/10.1159/000443646}}, doi = {{10.1159/000443646}}, volume = {{8}}, year = {{2016}}, }