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Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine

Kåredal, Monica LU ; Ahlfors, Stefan LU ; Lindh, Christian LU and Jönsson, Bo A LU (2002) Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics In Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics p.325-326
Abstract
The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Electrospray ionization (ESI), Multiple reaction monitoring (MRM), Hexahydrophthalic anhydride (HHPA), Ion spray voltage
in
Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics
pages
325 - 326
publisher
American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States
conference name
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
external identifiers
  • Scopus:2442710423
language
English
LU publication?
yes
id
d920d204-3f2e-434a-bcf0-4fbed75f052e (old id 611306)
date added to LUP
2007-11-28 09:01:20
date last changed
2016-10-13 04:43:34
@misc{d920d204-3f2e-434a-bcf0-4fbed75f052e,
  abstract     = {The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.},
  author       = {Kåredal, Monica and Ahlfors, Stefan and Lindh, Christian and Jönsson, Bo A},
  keyword      = {Electrospray ionization (ESI),Multiple reaction monitoring (MRM),Hexahydrophthalic anhydride (HHPA),Ion spray voltage},
  language     = {eng},
  pages        = {325--326},
  publisher    = {ARRAY(0x7922888)},
  series       = {Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics},
  title        = {Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine},
  year         = {2002},
}